Limits...
Vasculogenic conditioning of peripheral blood mononuclear cells promotes endothelial progenitor cell expansion and phenotype transition of anti-inflammatory macrophage and T lymphocyte to cells with regenerative potential.

Masuda H, Tanaka R, Fujimura S, Ishikawa M, Akimaru H, Shizuno T, Sato A, Okada Y, Iida Y, Itoh J, Itoh Y, Kamiguchi H, Kawamoto A, Asahara T - J Am Heart Assoc (2014)

Bottom Line: The resulting cells (QQMNCs) in EPC colony-forming assay generated significantly more definitive EPC colonies than PBMNCs.Using murine ischemic hindlimb models, the efficacy of QQMNC intramuscular transplantation (Tx) was compared to that of PBMNCTx, cultured "early EPC" Tx (eEPCTx), and granulocyte colony-stimulating factor mobilized CD34(+) cell Tx (GmCD34Tx).Laser Doppler imaging revealed the blood perfusion recovery in ischemic hindlimbs after QQMNCTx superior to after PBMNCTx and eEPCTx, but also earlier than after GmCD34Tx.

View Article: PubMed Central - PubMed

Affiliation: Department of Regenerative Medicine Science, Tokai University School of Medicine, Isehara, Japan (H.M., T.S., A.S., T.A.).

Show MeSH

Related in: MedlinePlus

Evaluation of vasculogenesis by transplanted cells in ischemic hindlimbs. A, The representative 2D and 3D images to assess vasculogenesis and angiogenesis in cell (GmCD34) transplanted ATM by 3D spectrum analysis using a confocal microscope. (Green) Mouse microvessels stained with isolectin B4‐FITC, (red) vasculogenic microvessels by transplanted human cells, stained with human specific anti‐CD31 antibody/Alexa 594, and (blue) nuclei stained with TOTO‐3. The unit of numbers in their images indicates micrometers (μm). B and E, The panels show the representative similar images in each treatment group. Cells were transplanted at 2×105 cells per ATM. Scale bar=20 μm. C and F, Microvessel counts/mm2 in each group. D and G, Percent (%) microvessel volume per 3D image cube (142.58×142.58×8 μm) in each group. *P<0.05; **P<0.01; ***P<0.001 versus IMDM control in (C, D, F, and G). #P<0.05; ##P<0.01; ###P<0.001 versus PBMNCTx in (C and D) or eEPCTx in (F and G). Each column on the graph represents a mean±SE. N=3 to 4 mice per group. ATM indicates anterior tibial muscle; eEPCTx, early endothelial progenitor cell transplantation; GmCD34, granulocyte colony‐stimulating factor mobilized CD34+ cell; PBMNCTx, peripheral blood mononuclear cell transplantation; QQMNC, quality and quantity control culture of mononuclear cells.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4309104&req=5

fig07: Evaluation of vasculogenesis by transplanted cells in ischemic hindlimbs. A, The representative 2D and 3D images to assess vasculogenesis and angiogenesis in cell (GmCD34) transplanted ATM by 3D spectrum analysis using a confocal microscope. (Green) Mouse microvessels stained with isolectin B4‐FITC, (red) vasculogenic microvessels by transplanted human cells, stained with human specific anti‐CD31 antibody/Alexa 594, and (blue) nuclei stained with TOTO‐3. The unit of numbers in their images indicates micrometers (μm). B and E, The panels show the representative similar images in each treatment group. Cells were transplanted at 2×105 cells per ATM. Scale bar=20 μm. C and F, Microvessel counts/mm2 in each group. D and G, Percent (%) microvessel volume per 3D image cube (142.58×142.58×8 μm) in each group. *P<0.05; **P<0.01; ***P<0.001 versus IMDM control in (C, D, F, and G). #P<0.05; ##P<0.01; ###P<0.001 versus PBMNCTx in (C and D) or eEPCTx in (F and G). Each column on the graph represents a mean±SE. N=3 to 4 mice per group. ATM indicates anterior tibial muscle; eEPCTx, early endothelial progenitor cell transplantation; GmCD34, granulocyte colony‐stimulating factor mobilized CD34+ cell; PBMNCTx, peripheral blood mononuclear cell transplantation; QQMNC, quality and quantity control culture of mononuclear cells.

Mentions: We performed in vivo experimenents to assess vasculogenic properties of transplanted cells (2×105 cells/mouse): to investigate whether and to what extent transplanted cells differentiate into endothelial cell forming vascular structure in the host tissue, using a confocal fluorescence microscope (Figure 7A; Video S1).


Vasculogenic conditioning of peripheral blood mononuclear cells promotes endothelial progenitor cell expansion and phenotype transition of anti-inflammatory macrophage and T lymphocyte to cells with regenerative potential.

Masuda H, Tanaka R, Fujimura S, Ishikawa M, Akimaru H, Shizuno T, Sato A, Okada Y, Iida Y, Itoh J, Itoh Y, Kamiguchi H, Kawamoto A, Asahara T - J Am Heart Assoc (2014)

Evaluation of vasculogenesis by transplanted cells in ischemic hindlimbs. A, The representative 2D and 3D images to assess vasculogenesis and angiogenesis in cell (GmCD34) transplanted ATM by 3D spectrum analysis using a confocal microscope. (Green) Mouse microvessels stained with isolectin B4‐FITC, (red) vasculogenic microvessels by transplanted human cells, stained with human specific anti‐CD31 antibody/Alexa 594, and (blue) nuclei stained with TOTO‐3. The unit of numbers in their images indicates micrometers (μm). B and E, The panels show the representative similar images in each treatment group. Cells were transplanted at 2×105 cells per ATM. Scale bar=20 μm. C and F, Microvessel counts/mm2 in each group. D and G, Percent (%) microvessel volume per 3D image cube (142.58×142.58×8 μm) in each group. *P<0.05; **P<0.01; ***P<0.001 versus IMDM control in (C, D, F, and G). #P<0.05; ##P<0.01; ###P<0.001 versus PBMNCTx in (C and D) or eEPCTx in (F and G). Each column on the graph represents a mean±SE. N=3 to 4 mice per group. ATM indicates anterior tibial muscle; eEPCTx, early endothelial progenitor cell transplantation; GmCD34, granulocyte colony‐stimulating factor mobilized CD34+ cell; PBMNCTx, peripheral blood mononuclear cell transplantation; QQMNC, quality and quantity control culture of mononuclear cells.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4309104&req=5

fig07: Evaluation of vasculogenesis by transplanted cells in ischemic hindlimbs. A, The representative 2D and 3D images to assess vasculogenesis and angiogenesis in cell (GmCD34) transplanted ATM by 3D spectrum analysis using a confocal microscope. (Green) Mouse microvessels stained with isolectin B4‐FITC, (red) vasculogenic microvessels by transplanted human cells, stained with human specific anti‐CD31 antibody/Alexa 594, and (blue) nuclei stained with TOTO‐3. The unit of numbers in their images indicates micrometers (μm). B and E, The panels show the representative similar images in each treatment group. Cells were transplanted at 2×105 cells per ATM. Scale bar=20 μm. C and F, Microvessel counts/mm2 in each group. D and G, Percent (%) microvessel volume per 3D image cube (142.58×142.58×8 μm) in each group. *P<0.05; **P<0.01; ***P<0.001 versus IMDM control in (C, D, F, and G). #P<0.05; ##P<0.01; ###P<0.001 versus PBMNCTx in (C and D) or eEPCTx in (F and G). Each column on the graph represents a mean±SE. N=3 to 4 mice per group. ATM indicates anterior tibial muscle; eEPCTx, early endothelial progenitor cell transplantation; GmCD34, granulocyte colony‐stimulating factor mobilized CD34+ cell; PBMNCTx, peripheral blood mononuclear cell transplantation; QQMNC, quality and quantity control culture of mononuclear cells.
Mentions: We performed in vivo experimenents to assess vasculogenic properties of transplanted cells (2×105 cells/mouse): to investigate whether and to what extent transplanted cells differentiate into endothelial cell forming vascular structure in the host tissue, using a confocal fluorescence microscope (Figure 7A; Video S1).

Bottom Line: The resulting cells (QQMNCs) in EPC colony-forming assay generated significantly more definitive EPC colonies than PBMNCs.Using murine ischemic hindlimb models, the efficacy of QQMNC intramuscular transplantation (Tx) was compared to that of PBMNCTx, cultured "early EPC" Tx (eEPCTx), and granulocyte colony-stimulating factor mobilized CD34(+) cell Tx (GmCD34Tx).Laser Doppler imaging revealed the blood perfusion recovery in ischemic hindlimbs after QQMNCTx superior to after PBMNCTx and eEPCTx, but also earlier than after GmCD34Tx.

View Article: PubMed Central - PubMed

Affiliation: Department of Regenerative Medicine Science, Tokai University School of Medicine, Isehara, Japan (H.M., T.S., A.S., T.A.).

Show MeSH
Related in: MedlinePlus