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Vasculogenic conditioning of peripheral blood mononuclear cells promotes endothelial progenitor cell expansion and phenotype transition of anti-inflammatory macrophage and T lymphocyte to cells with regenerative potential.

Masuda H, Tanaka R, Fujimura S, Ishikawa M, Akimaru H, Shizuno T, Sato A, Okada Y, Iida Y, Itoh J, Itoh Y, Kamiguchi H, Kawamoto A, Asahara T - J Am Heart Assoc (2014)

Bottom Line: The resulting cells (QQMNCs) in EPC colony-forming assay generated significantly more definitive EPC colonies than PBMNCs.Using murine ischemic hindlimb models, the efficacy of QQMNC intramuscular transplantation (Tx) was compared to that of PBMNCTx, cultured "early EPC" Tx (eEPCTx), and granulocyte colony-stimulating factor mobilized CD34(+) cell Tx (GmCD34Tx).Laser Doppler imaging revealed the blood perfusion recovery in ischemic hindlimbs after QQMNCTx superior to after PBMNCTx and eEPCTx, but also earlier than after GmCD34Tx.

View Article: PubMed Central - PubMed

Affiliation: Department of Regenerative Medicine Science, Tokai University School of Medicine, Isehara, Japan (H.M., T.S., A.S., T.A.).

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Assessment of angiogenesis and arteriogenesis in ischemic hindlimbs. A and D, Representative pictures of angiogenesis and arteriogenesis in ATM in each group. (a through c) in (A) and (a through d) in (D): mouse microvessels stained with isolectin B4‐FITC. (d through f) in (A) and (e through h) in (D): pericyte recruited microvessels stained with Cy3‐conjugated anti‐α‐SMA antibody. The graphs present the counts of microvessels in (B and E) and pericyte recruited microvessels in (c and f). *P<0.05; **P<0.01; ***P<0.001 versus IMDM control in (B, C, E, and F). #P<0.05; ###P<0.001 versus PBMNCTx in (B and C). #P<0.05; ##P<0.01 versus eEPCTx in (E and F). ×40 HPF. Each column in the graph represents a mean±SE. N=6 mice per group. ATM indicates anterior tibial muscle; eEPCTx, early endothelial progenitor cell transplantation; FITC, fluorescein isothiocyanate; GmCD34, granulocyte colony stimulating factor mobilized CD34+ cell; HPF, high power field; PBMNCTx, peripheral blood mononuclear cell transplantation; QQMNC, quality and quantity control culture of mononuclear cells; α‐SMA, alpha‐smooth muscle actin.
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fig06: Assessment of angiogenesis and arteriogenesis in ischemic hindlimbs. A and D, Representative pictures of angiogenesis and arteriogenesis in ATM in each group. (a through c) in (A) and (a through d) in (D): mouse microvessels stained with isolectin B4‐FITC. (d through f) in (A) and (e through h) in (D): pericyte recruited microvessels stained with Cy3‐conjugated anti‐α‐SMA antibody. The graphs present the counts of microvessels in (B and E) and pericyte recruited microvessels in (c and f). *P<0.05; **P<0.01; ***P<0.001 versus IMDM control in (B, C, E, and F). #P<0.05; ###P<0.001 versus PBMNCTx in (B and C). #P<0.05; ##P<0.01 versus eEPCTx in (E and F). ×40 HPF. Each column in the graph represents a mean±SE. N=6 mice per group. ATM indicates anterior tibial muscle; eEPCTx, early endothelial progenitor cell transplantation; FITC, fluorescein isothiocyanate; GmCD34, granulocyte colony stimulating factor mobilized CD34+ cell; HPF, high power field; PBMNCTx, peripheral blood mononuclear cell transplantation; QQMNC, quality and quantity control culture of mononuclear cells; α‐SMA, alpha‐smooth muscle actin.

Mentions: For each animal, we used histological methods to measure Isolectin B4‐FITC stained microvessel density and assess angiogenic capacity in the ATM of ischemic hindlimbs; the mean densities (microvessel counts/mm2) for the QQMNCTx, PBMNCTx, and control groups were 400.7±37.9, 118.9±20.1, and 98.7±15.8, respectively (Figure 6A and 6B).


Vasculogenic conditioning of peripheral blood mononuclear cells promotes endothelial progenitor cell expansion and phenotype transition of anti-inflammatory macrophage and T lymphocyte to cells with regenerative potential.

Masuda H, Tanaka R, Fujimura S, Ishikawa M, Akimaru H, Shizuno T, Sato A, Okada Y, Iida Y, Itoh J, Itoh Y, Kamiguchi H, Kawamoto A, Asahara T - J Am Heart Assoc (2014)

Assessment of angiogenesis and arteriogenesis in ischemic hindlimbs. A and D, Representative pictures of angiogenesis and arteriogenesis in ATM in each group. (a through c) in (A) and (a through d) in (D): mouse microvessels stained with isolectin B4‐FITC. (d through f) in (A) and (e through h) in (D): pericyte recruited microvessels stained with Cy3‐conjugated anti‐α‐SMA antibody. The graphs present the counts of microvessels in (B and E) and pericyte recruited microvessels in (c and f). *P<0.05; **P<0.01; ***P<0.001 versus IMDM control in (B, C, E, and F). #P<0.05; ###P<0.001 versus PBMNCTx in (B and C). #P<0.05; ##P<0.01 versus eEPCTx in (E and F). ×40 HPF. Each column in the graph represents a mean±SE. N=6 mice per group. ATM indicates anterior tibial muscle; eEPCTx, early endothelial progenitor cell transplantation; FITC, fluorescein isothiocyanate; GmCD34, granulocyte colony stimulating factor mobilized CD34+ cell; HPF, high power field; PBMNCTx, peripheral blood mononuclear cell transplantation; QQMNC, quality and quantity control culture of mononuclear cells; α‐SMA, alpha‐smooth muscle actin.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4309104&req=5

fig06: Assessment of angiogenesis and arteriogenesis in ischemic hindlimbs. A and D, Representative pictures of angiogenesis and arteriogenesis in ATM in each group. (a through c) in (A) and (a through d) in (D): mouse microvessels stained with isolectin B4‐FITC. (d through f) in (A) and (e through h) in (D): pericyte recruited microvessels stained with Cy3‐conjugated anti‐α‐SMA antibody. The graphs present the counts of microvessels in (B and E) and pericyte recruited microvessels in (c and f). *P<0.05; **P<0.01; ***P<0.001 versus IMDM control in (B, C, E, and F). #P<0.05; ###P<0.001 versus PBMNCTx in (B and C). #P<0.05; ##P<0.01 versus eEPCTx in (E and F). ×40 HPF. Each column in the graph represents a mean±SE. N=6 mice per group. ATM indicates anterior tibial muscle; eEPCTx, early endothelial progenitor cell transplantation; FITC, fluorescein isothiocyanate; GmCD34, granulocyte colony stimulating factor mobilized CD34+ cell; HPF, high power field; PBMNCTx, peripheral blood mononuclear cell transplantation; QQMNC, quality and quantity control culture of mononuclear cells; α‐SMA, alpha‐smooth muscle actin.
Mentions: For each animal, we used histological methods to measure Isolectin B4‐FITC stained microvessel density and assess angiogenic capacity in the ATM of ischemic hindlimbs; the mean densities (microvessel counts/mm2) for the QQMNCTx, PBMNCTx, and control groups were 400.7±37.9, 118.9±20.1, and 98.7±15.8, respectively (Figure 6A and 6B).

Bottom Line: The resulting cells (QQMNCs) in EPC colony-forming assay generated significantly more definitive EPC colonies than PBMNCs.Using murine ischemic hindlimb models, the efficacy of QQMNC intramuscular transplantation (Tx) was compared to that of PBMNCTx, cultured "early EPC" Tx (eEPCTx), and granulocyte colony-stimulating factor mobilized CD34(+) cell Tx (GmCD34Tx).Laser Doppler imaging revealed the blood perfusion recovery in ischemic hindlimbs after QQMNCTx superior to after PBMNCTx and eEPCTx, but also earlier than after GmCD34Tx.

View Article: PubMed Central - PubMed

Affiliation: Department of Regenerative Medicine Science, Tokai University School of Medicine, Isehara, Japan (H.M., T.S., A.S., T.A.).

Show MeSH
Related in: MedlinePlus