Limits...
Vasculogenic conditioning of peripheral blood mononuclear cells promotes endothelial progenitor cell expansion and phenotype transition of anti-inflammatory macrophage and T lymphocyte to cells with regenerative potential.

Masuda H, Tanaka R, Fujimura S, Ishikawa M, Akimaru H, Shizuno T, Sato A, Okada Y, Iida Y, Itoh J, Itoh Y, Kamiguchi H, Kawamoto A, Asahara T - J Am Heart Assoc (2014)

Bottom Line: The resulting cells (QQMNCs) in EPC colony-forming assay generated significantly more definitive EPC colonies than PBMNCs.Using murine ischemic hindlimb models, the efficacy of QQMNC intramuscular transplantation (Tx) was compared to that of PBMNCTx, cultured "early EPC" Tx (eEPCTx), and granulocyte colony-stimulating factor mobilized CD34(+) cell Tx (GmCD34Tx).Laser Doppler imaging revealed the blood perfusion recovery in ischemic hindlimbs after QQMNCTx superior to after PBMNCTx and eEPCTx, but also earlier than after GmCD34Tx.

View Article: PubMed Central - PubMed

Affiliation: Department of Regenerative Medicine Science, Tokai University School of Medicine, Isehara, Japan (H.M., T.S., A.S., T.A.).

Show MeSH

Related in: MedlinePlus

qRT‐PCR assay of PBMNCs and QQMNCs. A, Proangiogenic growth factors. B, Proangiogenic cytokines. C, Pro‐ or anti‐inflammatory cytokines. D, MMPs. *P<0.05; **P<0.01; ***P<0.001. Each graph column represents a mean±SE. N=8 volunteers. Ang indicates angiopoietin; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; IGF, insulin‐like growth factor; IL, interleukin; MMPs, matrix metalloproteinases; PBMNCs, peripheral blood mononuclear cells; QQMNCs, quality and quantity control culture of mononuclear cells; qRT‐PCR, quantitative real‐time polymerase chain reaction; TGF, transforming growth factor; TNF, tumor necrosis factor; VEGF, vascular endothelial growth factor.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4309104&req=5

fig03: qRT‐PCR assay of PBMNCs and QQMNCs. A, Proangiogenic growth factors. B, Proangiogenic cytokines. C, Pro‐ or anti‐inflammatory cytokines. D, MMPs. *P<0.05; **P<0.01; ***P<0.001. Each graph column represents a mean±SE. N=8 volunteers. Ang indicates angiopoietin; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; IGF, insulin‐like growth factor; IL, interleukin; MMPs, matrix metalloproteinases; PBMNCs, peripheral blood mononuclear cells; QQMNCs, quality and quantity control culture of mononuclear cells; qRT‐PCR, quantitative real‐time polymerase chain reaction; TGF, transforming growth factor; TNF, tumor necrosis factor; VEGF, vascular endothelial growth factor.

Mentions: Expression of genes encoding vascular regeneration factors, including VEGF‐B, angiopoietin‐1 (Ang‐1), leptin, IL‐8, IL‐10, and insulin‐like growth factor 1 (IGF‐1), was much higher in QQMNCs than in PBMNCs; the fold increases in QQMNCs versus PBMNCs were 4.2 for VEGF‐B, 2.4 for Ang‐1, 35.9 for leptin, 6.3 for IL‐8, 5.4 for IL‐10, and 21.2 for IGF‐1 (Figure 3A and 3B). Especially, VEGF‐B and Ang‐1 also induce vascular maturation,33–34 and IGF‐1 exerts myogenic potential.35


Vasculogenic conditioning of peripheral blood mononuclear cells promotes endothelial progenitor cell expansion and phenotype transition of anti-inflammatory macrophage and T lymphocyte to cells with regenerative potential.

Masuda H, Tanaka R, Fujimura S, Ishikawa M, Akimaru H, Shizuno T, Sato A, Okada Y, Iida Y, Itoh J, Itoh Y, Kamiguchi H, Kawamoto A, Asahara T - J Am Heart Assoc (2014)

qRT‐PCR assay of PBMNCs and QQMNCs. A, Proangiogenic growth factors. B, Proangiogenic cytokines. C, Pro‐ or anti‐inflammatory cytokines. D, MMPs. *P<0.05; **P<0.01; ***P<0.001. Each graph column represents a mean±SE. N=8 volunteers. Ang indicates angiopoietin; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; IGF, insulin‐like growth factor; IL, interleukin; MMPs, matrix metalloproteinases; PBMNCs, peripheral blood mononuclear cells; QQMNCs, quality and quantity control culture of mononuclear cells; qRT‐PCR, quantitative real‐time polymerase chain reaction; TGF, transforming growth factor; TNF, tumor necrosis factor; VEGF, vascular endothelial growth factor.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4309104&req=5

fig03: qRT‐PCR assay of PBMNCs and QQMNCs. A, Proangiogenic growth factors. B, Proangiogenic cytokines. C, Pro‐ or anti‐inflammatory cytokines. D, MMPs. *P<0.05; **P<0.01; ***P<0.001. Each graph column represents a mean±SE. N=8 volunteers. Ang indicates angiopoietin; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; IGF, insulin‐like growth factor; IL, interleukin; MMPs, matrix metalloproteinases; PBMNCs, peripheral blood mononuclear cells; QQMNCs, quality and quantity control culture of mononuclear cells; qRT‐PCR, quantitative real‐time polymerase chain reaction; TGF, transforming growth factor; TNF, tumor necrosis factor; VEGF, vascular endothelial growth factor.
Mentions: Expression of genes encoding vascular regeneration factors, including VEGF‐B, angiopoietin‐1 (Ang‐1), leptin, IL‐8, IL‐10, and insulin‐like growth factor 1 (IGF‐1), was much higher in QQMNCs than in PBMNCs; the fold increases in QQMNCs versus PBMNCs were 4.2 for VEGF‐B, 2.4 for Ang‐1, 35.9 for leptin, 6.3 for IL‐8, 5.4 for IL‐10, and 21.2 for IGF‐1 (Figure 3A and 3B). Especially, VEGF‐B and Ang‐1 also induce vascular maturation,33–34 and IGF‐1 exerts myogenic potential.35

Bottom Line: The resulting cells (QQMNCs) in EPC colony-forming assay generated significantly more definitive EPC colonies than PBMNCs.Using murine ischemic hindlimb models, the efficacy of QQMNC intramuscular transplantation (Tx) was compared to that of PBMNCTx, cultured "early EPC" Tx (eEPCTx), and granulocyte colony-stimulating factor mobilized CD34(+) cell Tx (GmCD34Tx).Laser Doppler imaging revealed the blood perfusion recovery in ischemic hindlimbs after QQMNCTx superior to after PBMNCTx and eEPCTx, but also earlier than after GmCD34Tx.

View Article: PubMed Central - PubMed

Affiliation: Department of Regenerative Medicine Science, Tokai University School of Medicine, Isehara, Japan (H.M., T.S., A.S., T.A.).

Show MeSH
Related in: MedlinePlus