Vasculogenic conditioning of peripheral blood mononuclear cells promotes endothelial progenitor cell expansion and phenotype transition of anti-inflammatory macrophage and T lymphocyte to cells with regenerative potential.
Bottom Line: The resulting cells (QQMNCs) in EPC colony-forming assay generated significantly more definitive EPC colonies than PBMNCs.Using murine ischemic hindlimb models, the efficacy of QQMNC intramuscular transplantation (Tx) was compared to that of PBMNCTx, cultured "early EPC" Tx (eEPCTx), and granulocyte colony-stimulating factor mobilized CD34(+) cell Tx (GmCD34Tx).Laser Doppler imaging revealed the blood perfusion recovery in ischemic hindlimbs after QQMNCTx superior to after PBMNCTx and eEPCTx, but also earlier than after GmCD34Tx.
Affiliation: Department of Regenerative Medicine Science, Tokai University School of Medicine, Isehara, Japan (H.M., T.S., A.S., T.A.).Show MeSH
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Mentions: Expression of genes encoding vascular regeneration factors, including VEGF‐B, angiopoietin‐1 (Ang‐1), leptin, IL‐8, IL‐10, and insulin‐like growth factor 1 (IGF‐1), was much higher in QQMNCs than in PBMNCs; the fold increases in QQMNCs versus PBMNCs were 4.2 for VEGF‐B, 2.4 for Ang‐1, 35.9 for leptin, 6.3 for IL‐8, 5.4 for IL‐10, and 21.2 for IGF‐1 (Figure 3A and 3B). Especially, VEGF‐B and Ang‐1 also induce vascular maturation,33–34 and IGF‐1 exerts myogenic potential.35
Affiliation: Department of Regenerative Medicine Science, Tokai University School of Medicine, Isehara, Japan (H.M., T.S., A.S., T.A.).