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Vasculogenic conditioning of peripheral blood mononuclear cells promotes endothelial progenitor cell expansion and phenotype transition of anti-inflammatory macrophage and T lymphocyte to cells with regenerative potential.

Masuda H, Tanaka R, Fujimura S, Ishikawa M, Akimaru H, Shizuno T, Sato A, Okada Y, Iida Y, Itoh J, Itoh Y, Kamiguchi H, Kawamoto A, Asahara T - J Am Heart Assoc (2014)

Bottom Line: The resulting cells (QQMNCs) in EPC colony-forming assay generated significantly more definitive EPC colonies than PBMNCs.Using murine ischemic hindlimb models, the efficacy of QQMNC intramuscular transplantation (Tx) was compared to that of PBMNCTx, cultured "early EPC" Tx (eEPCTx), and granulocyte colony-stimulating factor mobilized CD34(+) cell Tx (GmCD34Tx).Laser Doppler imaging revealed the blood perfusion recovery in ischemic hindlimbs after QQMNCTx superior to after PBMNCTx and eEPCTx, but also earlier than after GmCD34Tx.

View Article: PubMed Central - PubMed

Affiliation: Department of Regenerative Medicine Science, Tokai University School of Medicine, Isehara, Japan (H.M., T.S., A.S., T.A.).

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Histological evaluation of inflammation in ischemic hindlimbs. A and C, Representative pictures of inflammation in ATM by immunohistochemistry using anti‐iNOS antibody (brown color) in each group. ×20 HPF. (a) Control of rabbit polyclonal IgG, (b) IMDM control, (c) PBMNCTx, and (d) QQMNCTx in (A). (a) Control of rabbit polyclonal IgG, (b) IMDM control, (c) eEPCTx, (d) QQMNCTx, and (e) GmCD34Tx in (C). B and D, The graphs show percent (%) iNOS expressing area in each group. *P<0.05; ***P<0.001 versus IMDM control in (B and D). #P<0.05 versus PBMNCTx in (B) or eEPCTx in (D). Each graph column represents a mean±SE. N=6 mice per group. ATM indicates anterior tibial muscle; eEPCTx, early endothelial progenitor cell transplantation; GmCD34, granulocyte colony‐stimulating factor mobilized CD34+ cell; HPF, high power field; iNOS, inducible nitric oxide synthase; PBMNCTx, peripheral blood mononuclear cell transplantation; QQMNCTx, quality and quantity control culture of mononuclear cell transplantation.
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fig10: Histological evaluation of inflammation in ischemic hindlimbs. A and C, Representative pictures of inflammation in ATM by immunohistochemistry using anti‐iNOS antibody (brown color) in each group. ×20 HPF. (a) Control of rabbit polyclonal IgG, (b) IMDM control, (c) PBMNCTx, and (d) QQMNCTx in (A). (a) Control of rabbit polyclonal IgG, (b) IMDM control, (c) eEPCTx, (d) QQMNCTx, and (e) GmCD34Tx in (C). B and D, The graphs show percent (%) iNOS expressing area in each group. *P<0.05; ***P<0.001 versus IMDM control in (B and D). #P<0.05 versus PBMNCTx in (B) or eEPCTx in (D). Each graph column represents a mean±SE. N=6 mice per group. ATM indicates anterior tibial muscle; eEPCTx, early endothelial progenitor cell transplantation; GmCD34, granulocyte colony‐stimulating factor mobilized CD34+ cell; HPF, high power field; iNOS, inducible nitric oxide synthase; PBMNCTx, peripheral blood mononuclear cell transplantation; QQMNCTx, quality and quantity control culture of mononuclear cell transplantation.

Mentions: We performed IHC of iNOS in ischemic ATM to assess inflammation. The mean iNOS‐expressing areas (% iNOS‐expressing area/×20 HPF) for the QQMNCTx, PBMNCTx, and control groups were 3.16±0.58, 6.26±0.89, and 21.31±2.26 (Figure 10A and 10B). These findings indicate that QQMNCTx inhibited inflammation more markedly than PBMNCTx.


Vasculogenic conditioning of peripheral blood mononuclear cells promotes endothelial progenitor cell expansion and phenotype transition of anti-inflammatory macrophage and T lymphocyte to cells with regenerative potential.

Masuda H, Tanaka R, Fujimura S, Ishikawa M, Akimaru H, Shizuno T, Sato A, Okada Y, Iida Y, Itoh J, Itoh Y, Kamiguchi H, Kawamoto A, Asahara T - J Am Heart Assoc (2014)

Histological evaluation of inflammation in ischemic hindlimbs. A and C, Representative pictures of inflammation in ATM by immunohistochemistry using anti‐iNOS antibody (brown color) in each group. ×20 HPF. (a) Control of rabbit polyclonal IgG, (b) IMDM control, (c) PBMNCTx, and (d) QQMNCTx in (A). (a) Control of rabbit polyclonal IgG, (b) IMDM control, (c) eEPCTx, (d) QQMNCTx, and (e) GmCD34Tx in (C). B and D, The graphs show percent (%) iNOS expressing area in each group. *P<0.05; ***P<0.001 versus IMDM control in (B and D). #P<0.05 versus PBMNCTx in (B) or eEPCTx in (D). Each graph column represents a mean±SE. N=6 mice per group. ATM indicates anterior tibial muscle; eEPCTx, early endothelial progenitor cell transplantation; GmCD34, granulocyte colony‐stimulating factor mobilized CD34+ cell; HPF, high power field; iNOS, inducible nitric oxide synthase; PBMNCTx, peripheral blood mononuclear cell transplantation; QQMNCTx, quality and quantity control culture of mononuclear cell transplantation.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4309104&req=5

fig10: Histological evaluation of inflammation in ischemic hindlimbs. A and C, Representative pictures of inflammation in ATM by immunohistochemistry using anti‐iNOS antibody (brown color) in each group. ×20 HPF. (a) Control of rabbit polyclonal IgG, (b) IMDM control, (c) PBMNCTx, and (d) QQMNCTx in (A). (a) Control of rabbit polyclonal IgG, (b) IMDM control, (c) eEPCTx, (d) QQMNCTx, and (e) GmCD34Tx in (C). B and D, The graphs show percent (%) iNOS expressing area in each group. *P<0.05; ***P<0.001 versus IMDM control in (B and D). #P<0.05 versus PBMNCTx in (B) or eEPCTx in (D). Each graph column represents a mean±SE. N=6 mice per group. ATM indicates anterior tibial muscle; eEPCTx, early endothelial progenitor cell transplantation; GmCD34, granulocyte colony‐stimulating factor mobilized CD34+ cell; HPF, high power field; iNOS, inducible nitric oxide synthase; PBMNCTx, peripheral blood mononuclear cell transplantation; QQMNCTx, quality and quantity control culture of mononuclear cell transplantation.
Mentions: We performed IHC of iNOS in ischemic ATM to assess inflammation. The mean iNOS‐expressing areas (% iNOS‐expressing area/×20 HPF) for the QQMNCTx, PBMNCTx, and control groups were 3.16±0.58, 6.26±0.89, and 21.31±2.26 (Figure 10A and 10B). These findings indicate that QQMNCTx inhibited inflammation more markedly than PBMNCTx.

Bottom Line: The resulting cells (QQMNCs) in EPC colony-forming assay generated significantly more definitive EPC colonies than PBMNCs.Using murine ischemic hindlimb models, the efficacy of QQMNC intramuscular transplantation (Tx) was compared to that of PBMNCTx, cultured "early EPC" Tx (eEPCTx), and granulocyte colony-stimulating factor mobilized CD34(+) cell Tx (GmCD34Tx).Laser Doppler imaging revealed the blood perfusion recovery in ischemic hindlimbs after QQMNCTx superior to after PBMNCTx and eEPCTx, but also earlier than after GmCD34Tx.

View Article: PubMed Central - PubMed

Affiliation: Department of Regenerative Medicine Science, Tokai University School of Medicine, Isehara, Japan (H.M., T.S., A.S., T.A.).

Show MeSH
Related in: MedlinePlus