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Protective effect of a hydroethanolic extract from Bowdichia virgilioides on muscular damage and oxidative stress caused by strenuous resistance training in rats.

Dos Santos JL, Dantas RE, Lima CA, de Araújo SS, de Almeida EC, Marçal AC, Estevam Cdos S - J Int Soc Sports Nutr (2014)

Bottom Line: Treatment with B. virgilioides HEE or vehicle treatment was initiated after 25 days of RT (5 days; one dose per day).B. virgilioides HEE treatment reduced markers of oxidative stress caused by high-intensity RT.Further, HEE treatment during training significantly reduced the markers of tissue damage.

View Article: PubMed Central - PubMed

Affiliation: Postgraduate program in Physical Education, Department of Physical Education, Federal University of Sergipe, São Cristóvão, SE 49100-000 Brazil ; Federal University of Sergipe, Cidade Universitária Prof. José Aloísio de Campos, Department of Morphology, Av. Marechal Rondon s/n, Jardim Rosa Elze, São Cristóvão, Sergipe 49100-000 Brazil.

ABSTRACT

Background: Natural antioxidants can reduce oxidative damage caused by high-intensity resistance training (RT). We investigated the in vitro antioxidant potential of hydroethanolic extract (HEE) from Bowdichia virgilioides on muscular damage and oxidative stress in rats subjected to high-intensity RT.

Methods: Thirty-two male Wistar rats were divided into four experimental groups: 1) control group (CG), oral administration (P.O.) of vehicle; 2) trained group (TG), vehicle-treated with RT; 3) B. virgilioides untrained group (BVG), treated with B. virgilioides HEE (200 mg/kg P.O.); and 4) trained B. virgilioides group (TBVG), treated with B. virgiliodes HEE (200 mg/kg P.O.). All animals were habituated to the training apparatus for 1 week. CT and TBVG animals were subjected to the training protocol, which consisted of three sets of 10 repetitions with 75% of the load established using the one-repetition maximum, for four weeks. CG and BVG animals were manipulated and fixed to the apparatus three times a week with no load. Treatment with B. virgilioides HEE or vehicle treatment was initiated after 25 days of RT (5 days; one dose per day). At the end of the experiments, plasmatic and gastrocnemius samples from all groups were obtained for the assessment of lipid peroxidation and creatine kinase activity.

Results: Compared to TG rats, TBVG rats showed decreases in plasma and gastrocnemius tissue lipid peroxidation by 55.68% (p <0.0001) and 66.61% (p <0.0012), respectively. Further, compared to TG rats TBVG rats showed decreases in plasma and gastrocnemius tissue oxidative stress by 62.83% (p <0.0005) and 54.97% (p <0.0197), respectively.

Conclusions: B. virgilioides HEE treatment reduced markers of oxidative stress caused by high-intensity RT. Further, HEE treatment during training significantly reduced the markers of tissue damage.

No MeSH data available.


Related in: MedlinePlus

Effect of HEE (50 μg/mL) on lipid peroxidation induced by AAPH (A) and FeSO4(B). The results are shown as the concentration of malondialdehyde formed (nmol/mL). Values are expressed as the mean ± SD. Different letters on the graph stand for statistical difference between the groups. The statistical analysis was carried out using one-way ANOVA, followed by Bonferroni post-hoc test (p <0.05). All experiments were performed in triplicate.
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Fig3: Effect of HEE (50 μg/mL) on lipid peroxidation induced by AAPH (A) and FeSO4(B). The results are shown as the concentration of malondialdehyde formed (nmol/mL). Values are expressed as the mean ± SD. Different letters on the graph stand for statistical difference between the groups. The statistical analysis was carried out using one-way ANOVA, followed by Bonferroni post-hoc test (p <0.05). All experiments were performed in triplicate.

Mentions: The hydroethanolic extract of B. virgilioides inhibited AAPH- and iron sulfate-induced lipid peroxidation. HEE also showed potential as a chelating agent of transition metals and neutralized Fenton reactions. HEE inhibited AAPH- and iron sulfate-induced lipid peroxidation to a similar extent (p >0.05) as the positive control, Trolox (Figure 3).Figure 3


Protective effect of a hydroethanolic extract from Bowdichia virgilioides on muscular damage and oxidative stress caused by strenuous resistance training in rats.

Dos Santos JL, Dantas RE, Lima CA, de Araújo SS, de Almeida EC, Marçal AC, Estevam Cdos S - J Int Soc Sports Nutr (2014)

Effect of HEE (50 μg/mL) on lipid peroxidation induced by AAPH (A) and FeSO4(B). The results are shown as the concentration of malondialdehyde formed (nmol/mL). Values are expressed as the mean ± SD. Different letters on the graph stand for statistical difference between the groups. The statistical analysis was carried out using one-way ANOVA, followed by Bonferroni post-hoc test (p <0.05). All experiments were performed in triplicate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4308926&req=5

Fig3: Effect of HEE (50 μg/mL) on lipid peroxidation induced by AAPH (A) and FeSO4(B). The results are shown as the concentration of malondialdehyde formed (nmol/mL). Values are expressed as the mean ± SD. Different letters on the graph stand for statistical difference between the groups. The statistical analysis was carried out using one-way ANOVA, followed by Bonferroni post-hoc test (p <0.05). All experiments were performed in triplicate.
Mentions: The hydroethanolic extract of B. virgilioides inhibited AAPH- and iron sulfate-induced lipid peroxidation. HEE also showed potential as a chelating agent of transition metals and neutralized Fenton reactions. HEE inhibited AAPH- and iron sulfate-induced lipid peroxidation to a similar extent (p >0.05) as the positive control, Trolox (Figure 3).Figure 3

Bottom Line: Treatment with B. virgilioides HEE or vehicle treatment was initiated after 25 days of RT (5 days; one dose per day).B. virgilioides HEE treatment reduced markers of oxidative stress caused by high-intensity RT.Further, HEE treatment during training significantly reduced the markers of tissue damage.

View Article: PubMed Central - PubMed

Affiliation: Postgraduate program in Physical Education, Department of Physical Education, Federal University of Sergipe, São Cristóvão, SE 49100-000 Brazil ; Federal University of Sergipe, Cidade Universitária Prof. José Aloísio de Campos, Department of Morphology, Av. Marechal Rondon s/n, Jardim Rosa Elze, São Cristóvão, Sergipe 49100-000 Brazil.

ABSTRACT

Background: Natural antioxidants can reduce oxidative damage caused by high-intensity resistance training (RT). We investigated the in vitro antioxidant potential of hydroethanolic extract (HEE) from Bowdichia virgilioides on muscular damage and oxidative stress in rats subjected to high-intensity RT.

Methods: Thirty-two male Wistar rats were divided into four experimental groups: 1) control group (CG), oral administration (P.O.) of vehicle; 2) trained group (TG), vehicle-treated with RT; 3) B. virgilioides untrained group (BVG), treated with B. virgilioides HEE (200 mg/kg P.O.); and 4) trained B. virgilioides group (TBVG), treated with B. virgiliodes HEE (200 mg/kg P.O.). All animals were habituated to the training apparatus for 1 week. CT and TBVG animals were subjected to the training protocol, which consisted of three sets of 10 repetitions with 75% of the load established using the one-repetition maximum, for four weeks. CG and BVG animals were manipulated and fixed to the apparatus three times a week with no load. Treatment with B. virgilioides HEE or vehicle treatment was initiated after 25 days of RT (5 days; one dose per day). At the end of the experiments, plasmatic and gastrocnemius samples from all groups were obtained for the assessment of lipid peroxidation and creatine kinase activity.

Results: Compared to TG rats, TBVG rats showed decreases in plasma and gastrocnemius tissue lipid peroxidation by 55.68% (p <0.0001) and 66.61% (p <0.0012), respectively. Further, compared to TG rats TBVG rats showed decreases in plasma and gastrocnemius tissue oxidative stress by 62.83% (p <0.0005) and 54.97% (p <0.0197), respectively.

Conclusions: B. virgilioides HEE treatment reduced markers of oxidative stress caused by high-intensity RT. Further, HEE treatment during training significantly reduced the markers of tissue damage.

No MeSH data available.


Related in: MedlinePlus