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Adenovirus-mediated transfection with glucose transporter 3 suppresses PC12 cell apoptosis following ischemic injury.

Li J, Xu X, Zhang S, Zheng M, Wu Z, Weng Y, Ouyang L, Yu J, Li F - Neural Regen Res (2012)

Bottom Line: In this study, we investigated the effects of adenovirus-mediated transfection of PC12 cells with glucose transporter 3 after ischemic injury.The results of isotopic scintiscan and western blot assays showed that, the glucose uptake rate was significantly increased and nuclear factor kappaB expression was significantly decreased after adenovirus-mediated transfection of ischemic PC12 cells with glucose transporter 3.These results suggest that adenovirus-mediated transfection of cells with glucose transporter 3 elevates the energy metabolism of PC12 cells with ischemic injury, and inhibits cell apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, Guangdong Province, China.

ABSTRACT
In this study, we investigated the effects of adenovirus-mediated transfection of PC12 cells with glucose transporter 3 after ischemic injury. The results of flow cytometry and TUNEL showed that exogenous glucose transporter 3 significantly suppressed PC12 cell apoptosis induced by ischemic injury. The results of isotopic scintiscan and western blot assays showed that, the glucose uptake rate was significantly increased and nuclear factor kappaB expression was significantly decreased after adenovirus-mediated transfection of ischemic PC12 cells with glucose transporter 3. These results suggest that adenovirus-mediated transfection of cells with glucose transporter 3 elevates the energy metabolism of PC12 cells with ischemic injury, and inhibits cell apoptosis.

No MeSH data available.


Related in: MedlinePlus

Nuclear factor kappaB (NF-κB) expression in PC12 cells after ischemic injury (western blot assay).Blank control group: non-transfected PC12 cells; green fluorescent protein (GFP) group: GFP-transfected PC12 cells; glucose transporter 3 (GLUT3) group: GLUT3-transfected PC12 cells.
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Figure 3: Nuclear factor kappaB (NF-κB) expression in PC12 cells after ischemic injury (western blot assay).Blank control group: non-transfected PC12 cells; green fluorescent protein (GFP) group: GFP-transfected PC12 cells; glucose transporter 3 (GLUT3) group: GLUT3-transfected PC12 cells.

Mentions: The results of western blot assays showed that the level of NF-κB in GLUT3-transfected PC12 cells (0.92 ± 0.17) relative to β-actin was significantly lower than the level in GFP-transfected cells (1.02 ± 0.24) and non-transfected cells (1.06 ± 0.21) (P < 0.05; Figure 3).


Adenovirus-mediated transfection with glucose transporter 3 suppresses PC12 cell apoptosis following ischemic injury.

Li J, Xu X, Zhang S, Zheng M, Wu Z, Weng Y, Ouyang L, Yu J, Li F - Neural Regen Res (2012)

Nuclear factor kappaB (NF-κB) expression in PC12 cells after ischemic injury (western blot assay).Blank control group: non-transfected PC12 cells; green fluorescent protein (GFP) group: GFP-transfected PC12 cells; glucose transporter 3 (GLUT3) group: GLUT3-transfected PC12 cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308805&req=5

Figure 3: Nuclear factor kappaB (NF-κB) expression in PC12 cells after ischemic injury (western blot assay).Blank control group: non-transfected PC12 cells; green fluorescent protein (GFP) group: GFP-transfected PC12 cells; glucose transporter 3 (GLUT3) group: GLUT3-transfected PC12 cells.
Mentions: The results of western blot assays showed that the level of NF-κB in GLUT3-transfected PC12 cells (0.92 ± 0.17) relative to β-actin was significantly lower than the level in GFP-transfected cells (1.02 ± 0.24) and non-transfected cells (1.06 ± 0.21) (P < 0.05; Figure 3).

Bottom Line: In this study, we investigated the effects of adenovirus-mediated transfection of PC12 cells with glucose transporter 3 after ischemic injury.The results of isotopic scintiscan and western blot assays showed that, the glucose uptake rate was significantly increased and nuclear factor kappaB expression was significantly decreased after adenovirus-mediated transfection of ischemic PC12 cells with glucose transporter 3.These results suggest that adenovirus-mediated transfection of cells with glucose transporter 3 elevates the energy metabolism of PC12 cells with ischemic injury, and inhibits cell apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, Guangdong Province, China.

ABSTRACT
In this study, we investigated the effects of adenovirus-mediated transfection of PC12 cells with glucose transporter 3 after ischemic injury. The results of flow cytometry and TUNEL showed that exogenous glucose transporter 3 significantly suppressed PC12 cell apoptosis induced by ischemic injury. The results of isotopic scintiscan and western blot assays showed that, the glucose uptake rate was significantly increased and nuclear factor kappaB expression was significantly decreased after adenovirus-mediated transfection of ischemic PC12 cells with glucose transporter 3. These results suggest that adenovirus-mediated transfection of cells with glucose transporter 3 elevates the energy metabolism of PC12 cells with ischemic injury, and inhibits cell apoptosis.

No MeSH data available.


Related in: MedlinePlus