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Low levels of Bax inhibitor-1 gene expression increase tunicamycin-induced apoptosis in human neuroblastoma SY5Y cells.

Wu D, Wang P, Wang S - Neural Regen Res (2012)

Bottom Line: In control SH-SY5Y cells, tunicamycin treatment induced endoplasmic reticulum stress-mediated apoptosis; however, after Bax inhibitor-1 gene knockdown, cell survival rates were significantly decreased and the degree of apoptosis was significantly increased following tunicamycin treatment.In addition, chromatin condensation and apparent apoptotic phenomena, such as marginalization and cytoplasmic vesicles, were observed.Our findings indicate that Bax inhibitor-1 can delay apoptosis induced by endoplasmic reticulum stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Genetics, School of Basic Medical Sciences, Peking University, Beijing 100191, China.

ABSTRACT
A human SH-SY5Y neuroblastoma cell line with a low level of Bax inhibitor-1 expression was established by lentivirus-mediated RNA interference and fluorescence-activated cell sorting. In control SH-SY5Y cells, tunicamycin treatment induced endoplasmic reticulum stress-mediated apoptosis; however, after Bax inhibitor-1 gene knockdown, cell survival rates were significantly decreased and the degree of apoptosis was significantly increased following tunicamycin treatment. In addition, chromatin condensation and apparent apoptotic phenomena, such as marginalization and cytoplasmic vesicles, were observed. Our findings indicate that Bax inhibitor-1 can delay apoptosis induced by endoplasmic reticulum stress.

No MeSH data available.


Related in: MedlinePlus

Ultrastructure of SY5Y/P and SY5Y/B cells (electron microscopy).(A) Control cell line SY5Y/P without tunicamycin. The cells exhibit a lightly electron dense cytoplasm rich in organelles and are enclosed by an intact cell membrane, with clear nucleoli. Scale bar: 0.2 μm.(B) Control cell line SY5Y/P treated with 10 μg/mL tunicamycin. The cells exhibited membrane blebbing (arrowhead) and slightly condensed nuclear chromatin, with a normal nuclear membrane margin. Scale bar: 0.2 μm.(C) Most SY5Y/B cells showed invaginated nuclear membranes and increased cytoplasmic electron density even without tunicamycin treatment. Scale bar: 0.5 μm.(D) SY5Y/B cells exposed to 10 μg/mL tunicamycin show changes in features consistent with a later stage of apoptosis, including cytoplasmic vacuoles (small arrowhead), nuclear chromatin condensation, crystalline granular inclusion bodies (large arrowhead), absent nucleoli, and membrane blebbing. Scale bar: 0.2 μm.
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Figure 5: Ultrastructure of SY5Y/P and SY5Y/B cells (electron microscopy).(A) Control cell line SY5Y/P without tunicamycin. The cells exhibit a lightly electron dense cytoplasm rich in organelles and are enclosed by an intact cell membrane, with clear nucleoli. Scale bar: 0.2 μm.(B) Control cell line SY5Y/P treated with 10 μg/mL tunicamycin. The cells exhibited membrane blebbing (arrowhead) and slightly condensed nuclear chromatin, with a normal nuclear membrane margin. Scale bar: 0.2 μm.(C) Most SY5Y/B cells showed invaginated nuclear membranes and increased cytoplasmic electron density even without tunicamycin treatment. Scale bar: 0.5 μm.(D) SY5Y/B cells exposed to 10 μg/mL tunicamycin show changes in features consistent with a later stage of apoptosis, including cytoplasmic vacuoles (small arrowhead), nuclear chromatin condensation, crystalline granular inclusion bodies (large arrowhead), absent nucleoli, and membrane blebbing. Scale bar: 0.2 μm.

Mentions: Cell ultrastructure was observed by transmission electron microscopy (Figure 5). In the absence of TUN, SY5Y/B cells did not exhibit obvious morphological changes associated with apoptosis compared with SY5Y/P cells, although the SY5Y/B cells had shrunken but intact cell membranes. After TUN treatment, SY5Y/B cells underwent severe apoptosis with condensed, marginalized chromatin and vacuolated cytoplasm. The degree of apoptosis was significantly higher in SY5Y/B cells than in SY5Y/P cells.


Low levels of Bax inhibitor-1 gene expression increase tunicamycin-induced apoptosis in human neuroblastoma SY5Y cells.

Wu D, Wang P, Wang S - Neural Regen Res (2012)

Ultrastructure of SY5Y/P and SY5Y/B cells (electron microscopy).(A) Control cell line SY5Y/P without tunicamycin. The cells exhibit a lightly electron dense cytoplasm rich in organelles and are enclosed by an intact cell membrane, with clear nucleoli. Scale bar: 0.2 μm.(B) Control cell line SY5Y/P treated with 10 μg/mL tunicamycin. The cells exhibited membrane blebbing (arrowhead) and slightly condensed nuclear chromatin, with a normal nuclear membrane margin. Scale bar: 0.2 μm.(C) Most SY5Y/B cells showed invaginated nuclear membranes and increased cytoplasmic electron density even without tunicamycin treatment. Scale bar: 0.5 μm.(D) SY5Y/B cells exposed to 10 μg/mL tunicamycin show changes in features consistent with a later stage of apoptosis, including cytoplasmic vacuoles (small arrowhead), nuclear chromatin condensation, crystalline granular inclusion bodies (large arrowhead), absent nucleoli, and membrane blebbing. Scale bar: 0.2 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Figure 5: Ultrastructure of SY5Y/P and SY5Y/B cells (electron microscopy).(A) Control cell line SY5Y/P without tunicamycin. The cells exhibit a lightly electron dense cytoplasm rich in organelles and are enclosed by an intact cell membrane, with clear nucleoli. Scale bar: 0.2 μm.(B) Control cell line SY5Y/P treated with 10 μg/mL tunicamycin. The cells exhibited membrane blebbing (arrowhead) and slightly condensed nuclear chromatin, with a normal nuclear membrane margin. Scale bar: 0.2 μm.(C) Most SY5Y/B cells showed invaginated nuclear membranes and increased cytoplasmic electron density even without tunicamycin treatment. Scale bar: 0.5 μm.(D) SY5Y/B cells exposed to 10 μg/mL tunicamycin show changes in features consistent with a later stage of apoptosis, including cytoplasmic vacuoles (small arrowhead), nuclear chromatin condensation, crystalline granular inclusion bodies (large arrowhead), absent nucleoli, and membrane blebbing. Scale bar: 0.2 μm.
Mentions: Cell ultrastructure was observed by transmission electron microscopy (Figure 5). In the absence of TUN, SY5Y/B cells did not exhibit obvious morphological changes associated with apoptosis compared with SY5Y/P cells, although the SY5Y/B cells had shrunken but intact cell membranes. After TUN treatment, SY5Y/B cells underwent severe apoptosis with condensed, marginalized chromatin and vacuolated cytoplasm. The degree of apoptosis was significantly higher in SY5Y/B cells than in SY5Y/P cells.

Bottom Line: In control SH-SY5Y cells, tunicamycin treatment induced endoplasmic reticulum stress-mediated apoptosis; however, after Bax inhibitor-1 gene knockdown, cell survival rates were significantly decreased and the degree of apoptosis was significantly increased following tunicamycin treatment.In addition, chromatin condensation and apparent apoptotic phenomena, such as marginalization and cytoplasmic vesicles, were observed.Our findings indicate that Bax inhibitor-1 can delay apoptosis induced by endoplasmic reticulum stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Genetics, School of Basic Medical Sciences, Peking University, Beijing 100191, China.

ABSTRACT
A human SH-SY5Y neuroblastoma cell line with a low level of Bax inhibitor-1 expression was established by lentivirus-mediated RNA interference and fluorescence-activated cell sorting. In control SH-SY5Y cells, tunicamycin treatment induced endoplasmic reticulum stress-mediated apoptosis; however, after Bax inhibitor-1 gene knockdown, cell survival rates were significantly decreased and the degree of apoptosis was significantly increased following tunicamycin treatment. In addition, chromatin condensation and apparent apoptotic phenomena, such as marginalization and cytoplasmic vesicles, were observed. Our findings indicate that Bax inhibitor-1 can delay apoptosis induced by endoplasmic reticulum stress.

No MeSH data available.


Related in: MedlinePlus