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Apoptosis-related protein expression in rabbits with blast brain injury following early hyperbaric oxygen therapy.

Xu S, Liu J, Zhang Y, Wang C, Wang J, Yang Y, Huo J, Sun W - Neural Regen Res (2012)

Bottom Line: Expression of the apoptosis-regulating protein cytochrome c, the pro-apoptotic protein Bax and the apoptosis marker caspase-3 in the tissues surrounding the area of injury was significantly reduced, while that of the anti-apoptotic protein Bcl-2 was significantly increased.Our findings indicate that the curative effects of early hyperbaric oxygen on cortical cell apoptosis is associated with suppression of cytochrome c release from mitochondria.This mechanism underlies the observed reduction in Bax expression and upregulation of Bcl-2 expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, the 105 Hospital of Chinese PLA, Hefei 230031, Anhui Province, China.

ABSTRACT
We treated detonator-explosion-induced craniocerebral injury in rabbits with hyperbaric oxygen 1-24 hours post-injury. Expression of the apoptosis-regulating protein cytochrome c, the pro-apoptotic protein Bax and the apoptosis marker caspase-3 in the tissues surrounding the area of injury was significantly reduced, while that of the anti-apoptotic protein Bcl-2 was significantly increased. Our findings indicate that the curative effects of early hyperbaric oxygen on cortical cell apoptosis is associated with suppression of cytochrome c release from mitochondria. This mechanism underlies the observed reduction in Bax expression and upregulation of Bcl-2 expression.

No MeSH data available.


Related in: MedlinePlus

Caspase-3 expression in rabbit brain tissues around the injury site.(A) Immunohistochemical staining of caspase-3 expression in rabbit brain tissues around the injury site showed that caspase-3 was mainly expressed in the cytoplasm, appearing as brown-yellow particles (× 400).(B) Histogram of caspase-3 expression in rabbit brain tissues around the injury site. High absorbance value represents high level of caspase-3 expression. Data were expressed as mean ± SD. Intergroup comparison was conducted using the Student-Newman-Keuls method. aP < 0.05, vs. control group; bP < 0.05, vs. model group.HBOT: Hyperbaric oxygen treatment; h: hour; d: day.
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Figure 4: Caspase-3 expression in rabbit brain tissues around the injury site.(A) Immunohistochemical staining of caspase-3 expression in rabbit brain tissues around the injury site showed that caspase-3 was mainly expressed in the cytoplasm, appearing as brown-yellow particles (× 400).(B) Histogram of caspase-3 expression in rabbit brain tissues around the injury site. High absorbance value represents high level of caspase-3 expression. Data were expressed as mean ± SD. Intergroup comparison was conducted using the Student-Newman-Keuls method. aP < 0.05, vs. control group; bP < 0.05, vs. model group.HBOT: Hyperbaric oxygen treatment; h: hour; d: day.

Mentions: Caspase-3 activation is a marker of apoptosis in its irreversible stage[24], and, as such, is used as a major marker for apoptosis. Immunohistochemistry showed little caspase-3 expression in the cerebral cortex of control group rabbits. However, caspase-3 expression was detected in the brain tissues around the injury site 1 hour after the blast. Levels then gradually increased, peaked at 24 hours, gradually decreased and were restored to normal by day 14 (P > 0.05). Caspase-3 expression in brain tissues around the injury site was decreased in the early stage (1-24 hours post-blast injury) of HBOT (P < 0.05) but remained higher than the control group (P < 0.05), and was restored to normal levels by day 14 (P > 0.05; Figure 4).


Apoptosis-related protein expression in rabbits with blast brain injury following early hyperbaric oxygen therapy.

Xu S, Liu J, Zhang Y, Wang C, Wang J, Yang Y, Huo J, Sun W - Neural Regen Res (2012)

Caspase-3 expression in rabbit brain tissues around the injury site.(A) Immunohistochemical staining of caspase-3 expression in rabbit brain tissues around the injury site showed that caspase-3 was mainly expressed in the cytoplasm, appearing as brown-yellow particles (× 400).(B) Histogram of caspase-3 expression in rabbit brain tissues around the injury site. High absorbance value represents high level of caspase-3 expression. Data were expressed as mean ± SD. Intergroup comparison was conducted using the Student-Newman-Keuls method. aP < 0.05, vs. control group; bP < 0.05, vs. model group.HBOT: Hyperbaric oxygen treatment; h: hour; d: day.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308802&req=5

Figure 4: Caspase-3 expression in rabbit brain tissues around the injury site.(A) Immunohistochemical staining of caspase-3 expression in rabbit brain tissues around the injury site showed that caspase-3 was mainly expressed in the cytoplasm, appearing as brown-yellow particles (× 400).(B) Histogram of caspase-3 expression in rabbit brain tissues around the injury site. High absorbance value represents high level of caspase-3 expression. Data were expressed as mean ± SD. Intergroup comparison was conducted using the Student-Newman-Keuls method. aP < 0.05, vs. control group; bP < 0.05, vs. model group.HBOT: Hyperbaric oxygen treatment; h: hour; d: day.
Mentions: Caspase-3 activation is a marker of apoptosis in its irreversible stage[24], and, as such, is used as a major marker for apoptosis. Immunohistochemistry showed little caspase-3 expression in the cerebral cortex of control group rabbits. However, caspase-3 expression was detected in the brain tissues around the injury site 1 hour after the blast. Levels then gradually increased, peaked at 24 hours, gradually decreased and were restored to normal by day 14 (P > 0.05). Caspase-3 expression in brain tissues around the injury site was decreased in the early stage (1-24 hours post-blast injury) of HBOT (P < 0.05) but remained higher than the control group (P < 0.05), and was restored to normal levels by day 14 (P > 0.05; Figure 4).

Bottom Line: Expression of the apoptosis-regulating protein cytochrome c, the pro-apoptotic protein Bax and the apoptosis marker caspase-3 in the tissues surrounding the area of injury was significantly reduced, while that of the anti-apoptotic protein Bcl-2 was significantly increased.Our findings indicate that the curative effects of early hyperbaric oxygen on cortical cell apoptosis is associated with suppression of cytochrome c release from mitochondria.This mechanism underlies the observed reduction in Bax expression and upregulation of Bcl-2 expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, the 105 Hospital of Chinese PLA, Hefei 230031, Anhui Province, China.

ABSTRACT
We treated detonator-explosion-induced craniocerebral injury in rabbits with hyperbaric oxygen 1-24 hours post-injury. Expression of the apoptosis-regulating protein cytochrome c, the pro-apoptotic protein Bax and the apoptosis marker caspase-3 in the tissues surrounding the area of injury was significantly reduced, while that of the anti-apoptotic protein Bcl-2 was significantly increased. Our findings indicate that the curative effects of early hyperbaric oxygen on cortical cell apoptosis is associated with suppression of cytochrome c release from mitochondria. This mechanism underlies the observed reduction in Bax expression and upregulation of Bcl-2 expression.

No MeSH data available.


Related in: MedlinePlus