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Intercellular adhesion molecule-1 expression in the hippocampal CA1 region of hyperlipidemic rats with chronic cerebral ischemia.

Cheng Y, Zhang Y, Song H, Feng J - Neural Regen Res (2012)

Bottom Line: Intercellular adhesion molecule-1 expression in rat hippocampal CA1 region was determined to better understand the mechanism underlying the effects of hyperlipidemia on chronic cerebral ischemia.This correlated with pathological changes in the hippocampal CA1 region as detected by hematoxylin-eosin staining.These findings suggest that hyperlipidemia aggravates chronic cerebral ischemia-induced neurological damage and cognitive impairment in the rat hippocampal CA1 region, which may be mediated, at least in part, by up-regulated expression of intercellular adhesion molecule-1.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, The First Bethune Hospital of Jilin University, Changchun 130021, Jilin Province, China.

ABSTRACT
Chronic cerebral ischemia is a pathological process in many cerebrovascular diseases and it is induced by long-term hyperlipidemia, hypertension and diabetes mellitus. After being fed a high-fat diet for 4 weeks, rats were subjected to permanent occlusion of bilateral common carotid arteries to establish rat models of chronic cerebral ischemia with hyperlipidemia. Intercellular adhesion molecule-1 expression in rat hippocampal CA1 region was determined to better understand the mechanism underlying the effects of hyperlipidemia on chronic cerebral ischemia. Water maze test results showed that the cognitive function of rats with hyperlipidemia or chronic cerebral ischemia, particularly in rats with hyperlipidemia combined with chronic cerebral ischemia, gradually decreased between 1 and 4 months after occlusion of the bilateral common carotid arteries. This correlated with pathological changes in the hippocampal CA1 region as detected by hematoxylin-eosin staining. Immunohistochemical staining showed that intercellular adhesion molecule-1 expression in the hippocampal CA1 region was noticeably increased in rats with hyperlipidemia or chronic cerebral ischemia, in particular in rats with hyperlipidemia combined with chronic cerebral ischemia. These findings suggest that hyperlipidemia aggravates chronic cerebral ischemia-induced neurological damage and cognitive impairment in the rat hippocampal CA1 region, which may be mediated, at least in part, by up-regulated expression of intercellular adhesion molecule-1.

No MeSH data available.


Related in: MedlinePlus

The ICAM-1-positive cells in the hippocampal CA1 in the control, 2VO, HL and HL + 2VO groups at 1, 2 and 4 months post-surgery. Scale bar: 20 µm.Values are expressed as mean ± SD of five rats from each group. aP < 0.05, vs. other groups (Student's t-test); bP < 0.05, vs. the 1st month in the same group (Student's t-test); cP < 0.05, vs. other time points (Student's t-test). ICAM-1: Intracellular adhesion molecule 1; 2VO: Occlusion of bilateral common carotid arteries; HL: hyperlipidemia.
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Figure 3: The ICAM-1-positive cells in the hippocampal CA1 in the control, 2VO, HL and HL + 2VO groups at 1, 2 and 4 months post-surgery. Scale bar: 20 µm.Values are expressed as mean ± SD of five rats from each group. aP < 0.05, vs. other groups (Student's t-test); bP < 0.05, vs. the 1st month in the same group (Student's t-test); cP < 0.05, vs. other time points (Student's t-test). ICAM-1: Intracellular adhesion molecule 1; 2VO: Occlusion of bilateral common carotid arteries; HL: hyperlipidemia.

Mentions: In the control group, there were no ICAM-1-positive cells in the 1st month, and only a few in the 2nd and 4th months. In the 2VO, HL and HL + 2VO groups, a large number of positive cells were observed in the 1st month. The positive cells were considered to be pyramidal cells according to their shape and position, and most of them were stained weakly. Cell numbers increased between the 1st and 4th months. In the 2nd month, some positive cells were glial cells, and by the 4th month, most positive cells were glial cells, which stained strongly, and there were only a few pyramidal cells. At each time point, the HL + 2VO group had the largest number of ICAM-1-positive cells (P < 0.05; Figure 3).


Intercellular adhesion molecule-1 expression in the hippocampal CA1 region of hyperlipidemic rats with chronic cerebral ischemia.

Cheng Y, Zhang Y, Song H, Feng J - Neural Regen Res (2012)

The ICAM-1-positive cells in the hippocampal CA1 in the control, 2VO, HL and HL + 2VO groups at 1, 2 and 4 months post-surgery. Scale bar: 20 µm.Values are expressed as mean ± SD of five rats from each group. aP < 0.05, vs. other groups (Student's t-test); bP < 0.05, vs. the 1st month in the same group (Student's t-test); cP < 0.05, vs. other time points (Student's t-test). ICAM-1: Intracellular adhesion molecule 1; 2VO: Occlusion of bilateral common carotid arteries; HL: hyperlipidemia.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308801&req=5

Figure 3: The ICAM-1-positive cells in the hippocampal CA1 in the control, 2VO, HL and HL + 2VO groups at 1, 2 and 4 months post-surgery. Scale bar: 20 µm.Values are expressed as mean ± SD of five rats from each group. aP < 0.05, vs. other groups (Student's t-test); bP < 0.05, vs. the 1st month in the same group (Student's t-test); cP < 0.05, vs. other time points (Student's t-test). ICAM-1: Intracellular adhesion molecule 1; 2VO: Occlusion of bilateral common carotid arteries; HL: hyperlipidemia.
Mentions: In the control group, there were no ICAM-1-positive cells in the 1st month, and only a few in the 2nd and 4th months. In the 2VO, HL and HL + 2VO groups, a large number of positive cells were observed in the 1st month. The positive cells were considered to be pyramidal cells according to their shape and position, and most of them were stained weakly. Cell numbers increased between the 1st and 4th months. In the 2nd month, some positive cells were glial cells, and by the 4th month, most positive cells were glial cells, which stained strongly, and there were only a few pyramidal cells. At each time point, the HL + 2VO group had the largest number of ICAM-1-positive cells (P < 0.05; Figure 3).

Bottom Line: Intercellular adhesion molecule-1 expression in rat hippocampal CA1 region was determined to better understand the mechanism underlying the effects of hyperlipidemia on chronic cerebral ischemia.This correlated with pathological changes in the hippocampal CA1 region as detected by hematoxylin-eosin staining.These findings suggest that hyperlipidemia aggravates chronic cerebral ischemia-induced neurological damage and cognitive impairment in the rat hippocampal CA1 region, which may be mediated, at least in part, by up-regulated expression of intercellular adhesion molecule-1.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, The First Bethune Hospital of Jilin University, Changchun 130021, Jilin Province, China.

ABSTRACT
Chronic cerebral ischemia is a pathological process in many cerebrovascular diseases and it is induced by long-term hyperlipidemia, hypertension and diabetes mellitus. After being fed a high-fat diet for 4 weeks, rats were subjected to permanent occlusion of bilateral common carotid arteries to establish rat models of chronic cerebral ischemia with hyperlipidemia. Intercellular adhesion molecule-1 expression in rat hippocampal CA1 region was determined to better understand the mechanism underlying the effects of hyperlipidemia on chronic cerebral ischemia. Water maze test results showed that the cognitive function of rats with hyperlipidemia or chronic cerebral ischemia, particularly in rats with hyperlipidemia combined with chronic cerebral ischemia, gradually decreased between 1 and 4 months after occlusion of the bilateral common carotid arteries. This correlated with pathological changes in the hippocampal CA1 region as detected by hematoxylin-eosin staining. Immunohistochemical staining showed that intercellular adhesion molecule-1 expression in the hippocampal CA1 region was noticeably increased in rats with hyperlipidemia or chronic cerebral ischemia, in particular in rats with hyperlipidemia combined with chronic cerebral ischemia. These findings suggest that hyperlipidemia aggravates chronic cerebral ischemia-induced neurological damage and cognitive impairment in the rat hippocampal CA1 region, which may be mediated, at least in part, by up-regulated expression of intercellular adhesion molecule-1.

No MeSH data available.


Related in: MedlinePlus