Limits...
Perlecan and synaptophysin changes in denervated skeletal muscle.

Ma K, Huang Z, Ma J, Shao L, Wang H, Wang Y - Neural Regen Res (2012)

Bottom Line: Results showed degeneration and inflammation following transection of the sciatic nerve.With prolonged injury time (1-4 weeks post-injury), perlecan expression gradually decreased and reached the lowest level at 4 weeks, but synaptophysin expression remained unchanged after denervation.Results suggested that perlecan expression was more sensitive to denervation and reflected regional extracellular matrix changes following denervation.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Medical College of Zhejiang University, Hangzhou 310006, Zhejiang Province, China ; Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China.

ABSTRACT
The present study observed sciatic nerve and gastrocnemius muscle changes in denervated rats using morphology methods, and assessed expression of perlecan, an extracellular matrix component, which is located at the skeletal muscle cell surface as acetylcholine esterase, as well as synaptophysin, a synaptic marker. Results showed degeneration and inflammation following transection of the sciatic nerve. In addition, the sciatic nerve-dominated skeletal muscle degenerated with mild inflammation, indicating that skeletal muscle atrophy primarily contributed to denervation-induced nutritional disturbances. With prolonged injury time (1-4 weeks post-injury), perlecan expression gradually decreased and reached the lowest level at 4 weeks, but synaptophysin expression remained unchanged after denervation. Results suggested that perlecan expression was more sensitive to denervation and reflected regional extracellular matrix changes following denervation.

No MeSH data available.


Related in: MedlinePlus

Perlecan and synaptophysin expression in gastrocnemius muscle at different time points following denervation (immunohistochemical staining, light microscope, × 40).Positive products are stained brown yellow. With prolonged injury time, perlecan positive expression gradually decreases, but synaptophysin expression remains unchanged.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4308798&req=5

Figure 3: Perlecan and synaptophysin expression in gastrocnemius muscle at different time points following denervation (immunohistochemical staining, light microscope, × 40).Positive products are stained brown yellow. With prolonged injury time, perlecan positive expression gradually decreases, but synaptophysin expression remains unchanged.

Mentions: Immunohistochemical staining showed evenly distributed perlecan in a band-shape along the skeletal muscle cell membrane of normal skeletal muscle fibers. However, at 1 week post-injury, muscle cells shrank, and perlecan was released out of the cells and was unevenly distributed. At 2 weeks, perlecan expression significantly decreased, but was occasionally detected in the extracellular matrix surrounding skeletal muscle fibers. By 4 weeks, perlecan was not expressed, muscle cells further shrank, and skeletal muscle fibers space increased (Figure 3).


Perlecan and synaptophysin changes in denervated skeletal muscle.

Ma K, Huang Z, Ma J, Shao L, Wang H, Wang Y - Neural Regen Res (2012)

Perlecan and synaptophysin expression in gastrocnemius muscle at different time points following denervation (immunohistochemical staining, light microscope, × 40).Positive products are stained brown yellow. With prolonged injury time, perlecan positive expression gradually decreases, but synaptophysin expression remains unchanged.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308798&req=5

Figure 3: Perlecan and synaptophysin expression in gastrocnemius muscle at different time points following denervation (immunohistochemical staining, light microscope, × 40).Positive products are stained brown yellow. With prolonged injury time, perlecan positive expression gradually decreases, but synaptophysin expression remains unchanged.
Mentions: Immunohistochemical staining showed evenly distributed perlecan in a band-shape along the skeletal muscle cell membrane of normal skeletal muscle fibers. However, at 1 week post-injury, muscle cells shrank, and perlecan was released out of the cells and was unevenly distributed. At 2 weeks, perlecan expression significantly decreased, but was occasionally detected in the extracellular matrix surrounding skeletal muscle fibers. By 4 weeks, perlecan was not expressed, muscle cells further shrank, and skeletal muscle fibers space increased (Figure 3).

Bottom Line: Results showed degeneration and inflammation following transection of the sciatic nerve.With prolonged injury time (1-4 weeks post-injury), perlecan expression gradually decreased and reached the lowest level at 4 weeks, but synaptophysin expression remained unchanged after denervation.Results suggested that perlecan expression was more sensitive to denervation and reflected regional extracellular matrix changes following denervation.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Medical College of Zhejiang University, Hangzhou 310006, Zhejiang Province, China ; Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China.

ABSTRACT
The present study observed sciatic nerve and gastrocnemius muscle changes in denervated rats using morphology methods, and assessed expression of perlecan, an extracellular matrix component, which is located at the skeletal muscle cell surface as acetylcholine esterase, as well as synaptophysin, a synaptic marker. Results showed degeneration and inflammation following transection of the sciatic nerve. In addition, the sciatic nerve-dominated skeletal muscle degenerated with mild inflammation, indicating that skeletal muscle atrophy primarily contributed to denervation-induced nutritional disturbances. With prolonged injury time (1-4 weeks post-injury), perlecan expression gradually decreased and reached the lowest level at 4 weeks, but synaptophysin expression remained unchanged after denervation. Results suggested that perlecan expression was more sensitive to denervation and reflected regional extracellular matrix changes following denervation.

No MeSH data available.


Related in: MedlinePlus