Limits...
Perlecan and synaptophysin changes in denervated skeletal muscle.

Ma K, Huang Z, Ma J, Shao L, Wang H, Wang Y - Neural Regen Res (2012)

Bottom Line: Results showed degeneration and inflammation following transection of the sciatic nerve.With prolonged injury time (1-4 weeks post-injury), perlecan expression gradually decreased and reached the lowest level at 4 weeks, but synaptophysin expression remained unchanged after denervation.Results suggested that perlecan expression was more sensitive to denervation and reflected regional extracellular matrix changes following denervation.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Medical College of Zhejiang University, Hangzhou 310006, Zhejiang Province, China ; Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China.

ABSTRACT
The present study observed sciatic nerve and gastrocnemius muscle changes in denervated rats using morphology methods, and assessed expression of perlecan, an extracellular matrix component, which is located at the skeletal muscle cell surface as acetylcholine esterase, as well as synaptophysin, a synaptic marker. Results showed degeneration and inflammation following transection of the sciatic nerve. In addition, the sciatic nerve-dominated skeletal muscle degenerated with mild inflammation, indicating that skeletal muscle atrophy primarily contributed to denervation-induced nutritional disturbances. With prolonged injury time (1-4 weeks post-injury), perlecan expression gradually decreased and reached the lowest level at 4 weeks, but synaptophysin expression remained unchanged after denervation. Results suggested that perlecan expression was more sensitive to denervation and reflected regional extracellular matrix changes following denervation.

No MeSH data available.


Related in: MedlinePlus

Histological changes in the sciatic nerve proximal to the heart at 1 week post-injury (hematoxylin-eosin staining, light microscope, × 40).Retraction balls and lymphocyte infiltration are observed.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4308798&req=5

Figure 1: Histological changes in the sciatic nerve proximal to the heart at 1 week post-injury (hematoxylin-eosin staining, light microscope, × 40).Retraction balls and lymphocyte infiltration are observed.

Mentions: Hematoxylin-eosin staining revealed edema in the nerve stem, inflammatory cell infiltration surrounding the injury site, swollen neurites, and broken and degenerated neuritis at 1 week post-injury. After 2 weeks, myelin sheath degeneration was worse at the injury site, retraction balls formed proximal to the heart at the injury site to prevent cytoplasm loss, and the distal axons decreased or even disappeared. In addition, a large number of Schwann cells were observed, accompanied by a Bungner band and lymphocyte infiltration (Figure 1). After 4 weeks, inflammation around the injury site was significantly attenuated, the nerves remained swollen, and neurite flexion was obvious.


Perlecan and synaptophysin changes in denervated skeletal muscle.

Ma K, Huang Z, Ma J, Shao L, Wang H, Wang Y - Neural Regen Res (2012)

Histological changes in the sciatic nerve proximal to the heart at 1 week post-injury (hematoxylin-eosin staining, light microscope, × 40).Retraction balls and lymphocyte infiltration are observed.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308798&req=5

Figure 1: Histological changes in the sciatic nerve proximal to the heart at 1 week post-injury (hematoxylin-eosin staining, light microscope, × 40).Retraction balls and lymphocyte infiltration are observed.
Mentions: Hematoxylin-eosin staining revealed edema in the nerve stem, inflammatory cell infiltration surrounding the injury site, swollen neurites, and broken and degenerated neuritis at 1 week post-injury. After 2 weeks, myelin sheath degeneration was worse at the injury site, retraction balls formed proximal to the heart at the injury site to prevent cytoplasm loss, and the distal axons decreased or even disappeared. In addition, a large number of Schwann cells were observed, accompanied by a Bungner band and lymphocyte infiltration (Figure 1). After 4 weeks, inflammation around the injury site was significantly attenuated, the nerves remained swollen, and neurite flexion was obvious.

Bottom Line: Results showed degeneration and inflammation following transection of the sciatic nerve.With prolonged injury time (1-4 weeks post-injury), perlecan expression gradually decreased and reached the lowest level at 4 weeks, but synaptophysin expression remained unchanged after denervation.Results suggested that perlecan expression was more sensitive to denervation and reflected regional extracellular matrix changes following denervation.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Medical College of Zhejiang University, Hangzhou 310006, Zhejiang Province, China ; Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China.

ABSTRACT
The present study observed sciatic nerve and gastrocnemius muscle changes in denervated rats using morphology methods, and assessed expression of perlecan, an extracellular matrix component, which is located at the skeletal muscle cell surface as acetylcholine esterase, as well as synaptophysin, a synaptic marker. Results showed degeneration and inflammation following transection of the sciatic nerve. In addition, the sciatic nerve-dominated skeletal muscle degenerated with mild inflammation, indicating that skeletal muscle atrophy primarily contributed to denervation-induced nutritional disturbances. With prolonged injury time (1-4 weeks post-injury), perlecan expression gradually decreased and reached the lowest level at 4 weeks, but synaptophysin expression remained unchanged after denervation. Results suggested that perlecan expression was more sensitive to denervation and reflected regional extracellular matrix changes following denervation.

No MeSH data available.


Related in: MedlinePlus