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Gene expression profiling of the rat sciatic nerve in early Wallerian degeneration after injury.

Yao D, Li M, Shen D, Ding F, Lu S, Zhao Q, Gu X - Neural Regen Res (2012)

Bottom Line: Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed components involved in the Jak-STAT, ErbB, transforming growth factor-β, T cell receptor and calcium signaling pathways.Key factors included interleukin-6, interleukin-1, integrin, c-sarcoma, carcinoembryonic antigen-related cell adhesion molecules, chemokine (C-C motif) ligand, matrix metalloproteinase, BH3 interacting domain death agonist, baculoviral IAP repeat-containing 3 and Rac.The data were validated with real-time quantitative PCR.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226019, Jiangsu Province, China ; School of Life Sciences, Nantong University, Nantong 226019, Jiangsu Province, China.

ABSTRACT
Wallerian degeneration is an important area of research in modern neuroscience. A large number of genes are differentially regulated in the various stages of Wallerian degeneration, especially during the early response. In this study, we analyzed gene expression in early Wallerian degeneration of the distal nerve stump at 0, 0.5, 1, 6, 12 and 24 hours after rat sciatic nerve injury using gene chip microarrays. We screened for differentially-expressed genes and gene expression patterns. We examined the data for Gene Ontology, and explored the Kyoto Encyclopedia of Genes and Genomes Pathway. This allowed us to identify key regulatory factors and recurrent network motifs. We identified 1 546 differentially-expressed genes and 21 distinct patterns of gene expression in early Wallerian degeneration, and an enrichment of genes associated with the immune response, acute inflammation, apoptosis, cell adhesion, ion transport and the extracellular matrix. Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed components involved in the Jak-STAT, ErbB, transforming growth factor-β, T cell receptor and calcium signaling pathways. Key factors included interleukin-6, interleukin-1, integrin, c-sarcoma, carcinoembryonic antigen-related cell adhesion molecules, chemokine (C-C motif) ligand, matrix metalloproteinase, BH3 interacting domain death agonist, baculoviral IAP repeat-containing 3 and Rac. The data were validated with real-time quantitative PCR. This study provides a global view of gene expression profiles in early Wallerian degeneration of the rat sciatic nerve. Our findings provide insight into the molecular mechanisms underlying early Wallerian degeneration, and the regulation of nerve degeneration and regeneration.

No MeSH data available.


Related in: MedlinePlus

Real-time quantitative reverse transcription-PCR analysis of distal sciatic nerve stumps of rats at 0.5, 1, 6 and 12 hours post-surgery.The relative expression values of each mRNA were calculated using comparative Ct and were normalized using GAPDH for each data point.The average of three independent experiments is shown as mean ± SEM.
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Figure 5: Real-time quantitative reverse transcription-PCR analysis of distal sciatic nerve stumps of rats at 0.5, 1, 6 and 12 hours post-surgery.The relative expression values of each mRNA were calculated using comparative Ct and were normalized using GAPDH for each data point.The average of three independent experiments is shown as mean ± SEM.

Mentions: Although the reliability of our array data is demonstrated by a number of genes which have been previously described in response to peripheral nerve injury [such as c-jun (Herdegen and Zimmerman 1994), cyclin D1 (Kim et al. 2000), GADD45 (Befort et al. 2003), phospholipase A2 (Edstrm et al. 1996), PMP22 (Spreyer et al. 1991) and the p75NTR receptor (Heumann et al. 1987)], we have performed additional validation experiments for a number of genes, the expression or regulation of which has not been described in this context. We carried out real-time quantitative PCR for the following genes: BID, BIRC3, ITGA11, and ACVR1C. Real-time quantitative PCR reactions were run in triplicate. Levels of mRNAs were normalized against GAPDH. Expression levels for each time point were compared to the 0-hour time point and statistically analyzed (Figure 5). The real-time quantitative PCR results are consistent with the gene expression patterns identified by microarray analysis.


Gene expression profiling of the rat sciatic nerve in early Wallerian degeneration after injury.

Yao D, Li M, Shen D, Ding F, Lu S, Zhao Q, Gu X - Neural Regen Res (2012)

Real-time quantitative reverse transcription-PCR analysis of distal sciatic nerve stumps of rats at 0.5, 1, 6 and 12 hours post-surgery.The relative expression values of each mRNA were calculated using comparative Ct and were normalized using GAPDH for each data point.The average of three independent experiments is shown as mean ± SEM.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308797&req=5

Figure 5: Real-time quantitative reverse transcription-PCR analysis of distal sciatic nerve stumps of rats at 0.5, 1, 6 and 12 hours post-surgery.The relative expression values of each mRNA were calculated using comparative Ct and were normalized using GAPDH for each data point.The average of three independent experiments is shown as mean ± SEM.
Mentions: Although the reliability of our array data is demonstrated by a number of genes which have been previously described in response to peripheral nerve injury [such as c-jun (Herdegen and Zimmerman 1994), cyclin D1 (Kim et al. 2000), GADD45 (Befort et al. 2003), phospholipase A2 (Edstrm et al. 1996), PMP22 (Spreyer et al. 1991) and the p75NTR receptor (Heumann et al. 1987)], we have performed additional validation experiments for a number of genes, the expression or regulation of which has not been described in this context. We carried out real-time quantitative PCR for the following genes: BID, BIRC3, ITGA11, and ACVR1C. Real-time quantitative PCR reactions were run in triplicate. Levels of mRNAs were normalized against GAPDH. Expression levels for each time point were compared to the 0-hour time point and statistically analyzed (Figure 5). The real-time quantitative PCR results are consistent with the gene expression patterns identified by microarray analysis.

Bottom Line: Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed components involved in the Jak-STAT, ErbB, transforming growth factor-β, T cell receptor and calcium signaling pathways.Key factors included interleukin-6, interleukin-1, integrin, c-sarcoma, carcinoembryonic antigen-related cell adhesion molecules, chemokine (C-C motif) ligand, matrix metalloproteinase, BH3 interacting domain death agonist, baculoviral IAP repeat-containing 3 and Rac.The data were validated with real-time quantitative PCR.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226019, Jiangsu Province, China ; School of Life Sciences, Nantong University, Nantong 226019, Jiangsu Province, China.

ABSTRACT
Wallerian degeneration is an important area of research in modern neuroscience. A large number of genes are differentially regulated in the various stages of Wallerian degeneration, especially during the early response. In this study, we analyzed gene expression in early Wallerian degeneration of the distal nerve stump at 0, 0.5, 1, 6, 12 and 24 hours after rat sciatic nerve injury using gene chip microarrays. We screened for differentially-expressed genes and gene expression patterns. We examined the data for Gene Ontology, and explored the Kyoto Encyclopedia of Genes and Genomes Pathway. This allowed us to identify key regulatory factors and recurrent network motifs. We identified 1 546 differentially-expressed genes and 21 distinct patterns of gene expression in early Wallerian degeneration, and an enrichment of genes associated with the immune response, acute inflammation, apoptosis, cell adhesion, ion transport and the extracellular matrix. Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed components involved in the Jak-STAT, ErbB, transforming growth factor-β, T cell receptor and calcium signaling pathways. Key factors included interleukin-6, interleukin-1, integrin, c-sarcoma, carcinoembryonic antigen-related cell adhesion molecules, chemokine (C-C motif) ligand, matrix metalloproteinase, BH3 interacting domain death agonist, baculoviral IAP repeat-containing 3 and Rac. The data were validated with real-time quantitative PCR. This study provides a global view of gene expression profiles in early Wallerian degeneration of the rat sciatic nerve. Our findings provide insight into the molecular mechanisms underlying early Wallerian degeneration, and the regulation of nerve degeneration and regeneration.

No MeSH data available.


Related in: MedlinePlus