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Association of glutathione S-transferase T1 and M1 gene polymorphisms with ischemic stroke risk in the Chinese Han population.

Wang R, Wang Y, Wang J, Yang K - Neural Regen Res (2012)

Bottom Line: GSTT1 and GSTM1 genotypes were determined using polymerase chain reactions, electrophoresis and imaging analysis.No obvious evidence of GSTT1-, GSTM1- and GSTT1/GSTM1-double genotype distribution differences was found between case and control groups or between genders.Subgroup analysis showed that the risk of stroke was increased when hypertension was accompanied by GSTT1- (odds ratio (OR) = 2.996, P < 0.001) and GSTM1- (OR = 3.680, P < 0.001) genotypes; diabetes mellitus was accompanied by GSTT1- (OR = 1.860, P = 0.031) and GSTM1- (OR = 2.444, P = 0.002) genotypes, and smokers showed a GSTT1- genotype (OR = 2.276, P = 0.003).

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China.

ABSTRACT
Atherosclerosis plays an important role in ischemic stroke, and oxidative stress participates in the entire process of atherosclerosis. Glutathione S-transferase (GST) acting with other antioxidant enzymes can eliminate reactive oxygen species and protect cells against oxidative damage. To assess the association of glutathione S-transferase (GSTT1 and GSTM1) gene polymorphisms with ischemic stroke in the Chinese Han population, the present study selected 315 patients with ischemic stroke and 210 healthy controls for comparison. GSTT1 and GSTM1 genotypes were determined using polymerase chain reactions, electrophoresis and imaging analysis. No obvious evidence of GSTT1-, GSTM1- and GSTT1/GSTM1-double genotype distribution differences was found between case and control groups or between genders. Subgroup analysis showed that the risk of stroke was increased when hypertension was accompanied by GSTT1- (odds ratio (OR) = 2.996, P < 0.001) and GSTM1- (OR = 3.680, P < 0.001) genotypes; diabetes mellitus was accompanied by GSTT1- (OR = 1.860, P = 0.031) and GSTM1- (OR = 2.444, P = 0.002) genotypes, and smokers showed a GSTT1- genotype (OR = 2.276, P = 0.003). GSTT1- and GSTM1- genotypes may interact synergistically with hypertension, diabetes mellitus and smoking to increase the incidence risk of ischemic stroke.

No MeSH data available.


Related in: MedlinePlus

Electrophoretogram of GSTT1 and GSTM1 gene amplification products.M: Marker (100–600 bp); lanes 1–3 represent sample “a” (GSTM1+/GSTT1+), lanes 4–6 represent sample “b” (GSTM1+/GSTT1–), lanes 7–9 represent sample “c” (GSTM1–/GSTT1+), lanes 10–12 represent sample “d” (GSTM1–/GSTT1–). GSTM1(+) and GSTM1(+/–) were detected as a fragment at 219 bp, CYP1A1 exon7 was the fragment at 312 bp, GSTT1 (+) and GSTT1(+/-) was the fragment at 459 bp; GSTM1 (–) and GSTT1 (–) were detected as no corresponding fragment. GST: Glutathione S-transferase.Heterozygous deletion and the present genotype could not be distinguished in the electrophoretogram.
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Figure 1: Electrophoretogram of GSTT1 and GSTM1 gene amplification products.M: Marker (100–600 bp); lanes 1–3 represent sample “a” (GSTM1+/GSTT1+), lanes 4–6 represent sample “b” (GSTM1+/GSTT1–), lanes 7–9 represent sample “c” (GSTM1–/GSTT1+), lanes 10–12 represent sample “d” (GSTM1–/GSTT1–). GSTM1(+) and GSTM1(+/–) were detected as a fragment at 219 bp, CYP1A1 exon7 was the fragment at 312 bp, GSTT1 (+) and GSTT1(+/-) was the fragment at 459 bp; GSTM1 (–) and GSTT1 (–) were detected as no corresponding fragment. GST: Glutathione S-transferase.Heterozygous deletion and the present genotype could not be distinguished in the electrophoretogram.

Mentions: PCR and electrophoresis were used for gene amplification and genotyping. The results are shown in Figure 1.


Association of glutathione S-transferase T1 and M1 gene polymorphisms with ischemic stroke risk in the Chinese Han population.

Wang R, Wang Y, Wang J, Yang K - Neural Regen Res (2012)

Electrophoretogram of GSTT1 and GSTM1 gene amplification products.M: Marker (100–600 bp); lanes 1–3 represent sample “a” (GSTM1+/GSTT1+), lanes 4–6 represent sample “b” (GSTM1+/GSTT1–), lanes 7–9 represent sample “c” (GSTM1–/GSTT1+), lanes 10–12 represent sample “d” (GSTM1–/GSTT1–). GSTM1(+) and GSTM1(+/–) were detected as a fragment at 219 bp, CYP1A1 exon7 was the fragment at 312 bp, GSTT1 (+) and GSTT1(+/-) was the fragment at 459 bp; GSTM1 (–) and GSTT1 (–) were detected as no corresponding fragment. GST: Glutathione S-transferase.Heterozygous deletion and the present genotype could not be distinguished in the electrophoretogram.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308794&req=5

Figure 1: Electrophoretogram of GSTT1 and GSTM1 gene amplification products.M: Marker (100–600 bp); lanes 1–3 represent sample “a” (GSTM1+/GSTT1+), lanes 4–6 represent sample “b” (GSTM1+/GSTT1–), lanes 7–9 represent sample “c” (GSTM1–/GSTT1+), lanes 10–12 represent sample “d” (GSTM1–/GSTT1–). GSTM1(+) and GSTM1(+/–) were detected as a fragment at 219 bp, CYP1A1 exon7 was the fragment at 312 bp, GSTT1 (+) and GSTT1(+/-) was the fragment at 459 bp; GSTM1 (–) and GSTT1 (–) were detected as no corresponding fragment. GST: Glutathione S-transferase.Heterozygous deletion and the present genotype could not be distinguished in the electrophoretogram.
Mentions: PCR and electrophoresis were used for gene amplification and genotyping. The results are shown in Figure 1.

Bottom Line: GSTT1 and GSTM1 genotypes were determined using polymerase chain reactions, electrophoresis and imaging analysis.No obvious evidence of GSTT1-, GSTM1- and GSTT1/GSTM1-double genotype distribution differences was found between case and control groups or between genders.Subgroup analysis showed that the risk of stroke was increased when hypertension was accompanied by GSTT1- (odds ratio (OR) = 2.996, P < 0.001) and GSTM1- (OR = 3.680, P < 0.001) genotypes; diabetes mellitus was accompanied by GSTT1- (OR = 1.860, P = 0.031) and GSTM1- (OR = 2.444, P = 0.002) genotypes, and smokers showed a GSTT1- genotype (OR = 2.276, P = 0.003).

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China.

ABSTRACT
Atherosclerosis plays an important role in ischemic stroke, and oxidative stress participates in the entire process of atherosclerosis. Glutathione S-transferase (GST) acting with other antioxidant enzymes can eliminate reactive oxygen species and protect cells against oxidative damage. To assess the association of glutathione S-transferase (GSTT1 and GSTM1) gene polymorphisms with ischemic stroke in the Chinese Han population, the present study selected 315 patients with ischemic stroke and 210 healthy controls for comparison. GSTT1 and GSTM1 genotypes were determined using polymerase chain reactions, electrophoresis and imaging analysis. No obvious evidence of GSTT1-, GSTM1- and GSTT1/GSTM1-double genotype distribution differences was found between case and control groups or between genders. Subgroup analysis showed that the risk of stroke was increased when hypertension was accompanied by GSTT1- (odds ratio (OR) = 2.996, P < 0.001) and GSTM1- (OR = 3.680, P < 0.001) genotypes; diabetes mellitus was accompanied by GSTT1- (OR = 1.860, P = 0.031) and GSTM1- (OR = 2.444, P = 0.002) genotypes, and smokers showed a GSTT1- genotype (OR = 2.276, P = 0.003). GSTT1- and GSTM1- genotypes may interact synergistically with hypertension, diabetes mellitus and smoking to increase the incidence risk of ischemic stroke.

No MeSH data available.


Related in: MedlinePlus