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Traditional Chinese medicine monomers and compounds induce differentiation of stem cells into neuron-like cells

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Totally three articles focusing on inductive effects of gastrodin, active principle region of Buyang Huanwu decoction, and Xuefuzhuyu injection on differentiation of stem cells into neuron-like cells were published in three issues... Neural stem cells, isolated from newborn Wistar rats, were cultured in vitro and exposed to 100-4 000 μM kainic acid for 7 days to induce neuronal cell differentiation, causing the number of astrocytes to be significantly increased... Treatment with a combination of 0.5 mg/L gastrodin and kainic acid also caused the number of differentiated neurons to be significantly increased compared with treatment with kainic acid alone... After 28 days, nestin and neuron-specific enolase were expressed in the cytoplasm... Reverse transcription-PCR and western blot analyses showed that nestin and neuron-specific enolase mRNA and protein expression was greater in the active principle region group compared with the original formula group... Results demonstrated that the active principle region of Buyang Huanwu decoction induced greater differentiation of rat bone marrow-derived mesenchymal stem cells into neural-like cells in vitro than the original Buyang Huanwu decoction formula... The primary effective components of Xuefuzhuyu, including Rhizoma Chqanxiong, Radix Salviae Miltiorrhiae, and Radix Angelicae Sinensis, have been shown to induce the differentiation of bone marrow mesenchymal stem cells (BMMSCs) into neuronal-like cells... However, there is little available evidence on the effects of the Chinese herbal compound Xuefuzhuyu on BMMSC differentiation... Inverted microscopy was used to observe BMMSCs induced by Xuefuzhuyu; immunocytochemistry revealed expression of the neuronal marker neuron-specific enolase surface antigen in the majority of BMMSCs following treatment with Xuefuzhuyu at concentrations of 1, 3, 5, and 10 g/L... A concentration of 3 g/L resulted in highest neuron-specific enolase expression... These results suggest that BMMSCs can be induced to differentiate into neuronal-like cells with Xuefuzhuyu, and 3 g/L is the optimal inductive concentration for effective differentiation.

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Gastrodin blocks neural stem cell differentiation into glial cells mediated by kainic acidNeural Regen Res. 2012;7(12):891-895.
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Figure 1: Gastrodin blocks neural stem cell differentiation into glial cells mediated by kainic acidNeural Regen Res. 2012;7(12):891-895.


Traditional Chinese medicine monomers and compounds induce differentiation of stem cells into neuron-like cells
Gastrodin blocks neural stem cell differentiation into glial cells mediated by kainic acidNeural Regen Res. 2012;7(12):891-895.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308792&req=5

Figure 1: Gastrodin blocks neural stem cell differentiation into glial cells mediated by kainic acidNeural Regen Res. 2012;7(12):891-895.

View Article: PubMed Central

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

Totally three articles focusing on inductive effects of gastrodin, active principle region of Buyang Huanwu decoction, and Xuefuzhuyu injection on differentiation of stem cells into neuron-like cells were published in three issues... Neural stem cells, isolated from newborn Wistar rats, were cultured in vitro and exposed to 100-4 000 μM kainic acid for 7 days to induce neuronal cell differentiation, causing the number of astrocytes to be significantly increased... Treatment with a combination of 0.5 mg/L gastrodin and kainic acid also caused the number of differentiated neurons to be significantly increased compared with treatment with kainic acid alone... After 28 days, nestin and neuron-specific enolase were expressed in the cytoplasm... Reverse transcription-PCR and western blot analyses showed that nestin and neuron-specific enolase mRNA and protein expression was greater in the active principle region group compared with the original formula group... Results demonstrated that the active principle region of Buyang Huanwu decoction induced greater differentiation of rat bone marrow-derived mesenchymal stem cells into neural-like cells in vitro than the original Buyang Huanwu decoction formula... The primary effective components of Xuefuzhuyu, including Rhizoma Chqanxiong, Radix Salviae Miltiorrhiae, and Radix Angelicae Sinensis, have been shown to induce the differentiation of bone marrow mesenchymal stem cells (BMMSCs) into neuronal-like cells... However, there is little available evidence on the effects of the Chinese herbal compound Xuefuzhuyu on BMMSC differentiation... Inverted microscopy was used to observe BMMSCs induced by Xuefuzhuyu; immunocytochemistry revealed expression of the neuronal marker neuron-specific enolase surface antigen in the majority of BMMSCs following treatment with Xuefuzhuyu at concentrations of 1, 3, 5, and 10 g/L... A concentration of 3 g/L resulted in highest neuron-specific enolase expression... These results suggest that BMMSCs can be induced to differentiate into neuronal-like cells with Xuefuzhuyu, and 3 g/L is the optimal inductive concentration for effective differentiation.

No MeSH data available.