Limits...
The 9L(LUC)/Wistar rat glioma model is not suitable for immunotherapy.

Yang L, Zhao J, Zhou G, Wang Y, Li L, Yuan H, Nan X, Guan L, Pei X - Neural Regen Res (2012)

Bottom Line: The availability of a well-characterized animal brain tumor model will play an important role in identifying treatments for human brain tumors.Immunohistochemistry results demonstrated that no CD4- and CD8-positive cells were found in the syngeneic 9L(LUC)/F344 model.However, many infiltrating CD4- and CD8-positive cells were observed within the tumors of the 9L(LUC)/Wistar model.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, the 263 Hospital, Beijing 101149, China ; Department of Neurology, South West Hospital, the Third Military Medical University of Chinese PLA, Chongqing 400038, China.

ABSTRACT
The availability of a well-characterized animal brain tumor model will play an important role in identifying treatments for human brain tumors. Wistar rats bearing 9L glioma cells can develop solid, well-circumcised tumors, and may be a useful animal model for the evaluation of various therapeutic approaches for gliosarcomas. In this study, the 9L/Wistar rat glioma model was produced by intracerebral implantation of 9L(LUC) glioma cells syngenic to Fischer 344 (F344) rats. Bioluminescence imaging showed that tumors progressively grew from day 7 to day 21 in 9L(LUC)/F344 rats, and tumor regression was found in some 9L(LUC)/Wistar rats. Hematoxylin-eosin staining verified that intracranial tumors were gliomas. Immunohistochemistry results demonstrated that no CD4- and CD8-positive cells were found in the syngeneic 9L(LUC)/F344 model. However, many infiltrating CD4- and CD8-positive cells were observed within the tumors of the 9L(LUC)/Wistar model. Our data suggests that compared with 9L/F344 rats, 9L glioma Wistar rats may not be suitable for evaluating brain glioma immunotherapies, even though the model induced an immune response and exhibited tumor regression.

No MeSH data available.


Related in: MedlinePlus

Histopathological changes in the 9L/Wistar rat model, which expressed well-circumscribed gliomas (A), neovascularization (B), hemorrhaging (C) and pleomorphic growth (D) (Scale bars: 20 μm in A–C; × 400 in D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4308791&req=5

Figure 4: Histopathological changes in the 9L/Wistar rat model, which expressed well-circumscribed gliomas (A), neovascularization (B), hemorrhaging (C) and pleomorphic growth (D) (Scale bars: 20 μm in A–C; × 400 in D).

Mentions: At 7 days after implantation, all experimental animals including F344 and Wistar rats developed solid tumors. Margins at the tumor-brain interface were macroscopically demarcated. Histologically, tumors were hypercellular with neovascularization and hemorrhaging. Light microscopy revealed that tumors exhibited malignant characteristics, including pleomorphic growth, more than one nucleus per cell, prominent nucleoli or more than three distinct nucleoli, and a nuclear to cytoplasm ratio of > 0.5 (Figure 4).


The 9L(LUC)/Wistar rat glioma model is not suitable for immunotherapy.

Yang L, Zhao J, Zhou G, Wang Y, Li L, Yuan H, Nan X, Guan L, Pei X - Neural Regen Res (2012)

Histopathological changes in the 9L/Wistar rat model, which expressed well-circumscribed gliomas (A), neovascularization (B), hemorrhaging (C) and pleomorphic growth (D) (Scale bars: 20 μm in A–C; × 400 in D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308791&req=5

Figure 4: Histopathological changes in the 9L/Wistar rat model, which expressed well-circumscribed gliomas (A), neovascularization (B), hemorrhaging (C) and pleomorphic growth (D) (Scale bars: 20 μm in A–C; × 400 in D).
Mentions: At 7 days after implantation, all experimental animals including F344 and Wistar rats developed solid tumors. Margins at the tumor-brain interface were macroscopically demarcated. Histologically, tumors were hypercellular with neovascularization and hemorrhaging. Light microscopy revealed that tumors exhibited malignant characteristics, including pleomorphic growth, more than one nucleus per cell, prominent nucleoli or more than three distinct nucleoli, and a nuclear to cytoplasm ratio of > 0.5 (Figure 4).

Bottom Line: The availability of a well-characterized animal brain tumor model will play an important role in identifying treatments for human brain tumors.Immunohistochemistry results demonstrated that no CD4- and CD8-positive cells were found in the syngeneic 9L(LUC)/F344 model.However, many infiltrating CD4- and CD8-positive cells were observed within the tumors of the 9L(LUC)/Wistar model.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, the 263 Hospital, Beijing 101149, China ; Department of Neurology, South West Hospital, the Third Military Medical University of Chinese PLA, Chongqing 400038, China.

ABSTRACT
The availability of a well-characterized animal brain tumor model will play an important role in identifying treatments for human brain tumors. Wistar rats bearing 9L glioma cells can develop solid, well-circumcised tumors, and may be a useful animal model for the evaluation of various therapeutic approaches for gliosarcomas. In this study, the 9L/Wistar rat glioma model was produced by intracerebral implantation of 9L(LUC) glioma cells syngenic to Fischer 344 (F344) rats. Bioluminescence imaging showed that tumors progressively grew from day 7 to day 21 in 9L(LUC)/F344 rats, and tumor regression was found in some 9L(LUC)/Wistar rats. Hematoxylin-eosin staining verified that intracranial tumors were gliomas. Immunohistochemistry results demonstrated that no CD4- and CD8-positive cells were found in the syngeneic 9L(LUC)/F344 model. However, many infiltrating CD4- and CD8-positive cells were observed within the tumors of the 9L(LUC)/Wistar model. Our data suggests that compared with 9L/F344 rats, 9L glioma Wistar rats may not be suitable for evaluating brain glioma immunotherapies, even though the model induced an immune response and exhibited tumor regression.

No MeSH data available.


Related in: MedlinePlus