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The cumulative analgesic effect of repeated electroacupuncture involves synaptic remodeling in the hippocampal CA3 region.

Xu Q, Liu T, Chen S, Gao Y, Wang J, Qiao L, Liu J - Neural Regen Res (2012)

Bottom Line: In addition, concomitant changes in calcium/calmodulin-dependent protein kinase II expression and synaptic ultrastructure of neurons in the hippocampal CA3 region were examined.In ovariectomized rats with chronic constriction injury, the analgesic effect was significantly reduced.Repeated electroacupuncture intervention had a cumulative analgesic effect on injury-induced neuropathic pain reactions, and it led to synaptic remodeling of hippocampal neurons and upregulated calcium/calmodulin-dependent protein kinase II expression in the hippocampal CA3 region.

View Article: PubMed Central - PubMed

Affiliation: Hainan Medical University, Haikou 571101, Hainan Province, China.

ABSTRACT
In the present study, we examined the analgesic effect of repeated electroacupuncture at bilateral Zusanli (ST36) and Yanglingquan (GB34) once a day for 14 consecutive days in a rat model of chronic sciatic nerve constriction injury-induced neuropathic pain. In addition, concomitant changes in calcium/calmodulin-dependent protein kinase II expression and synaptic ultrastructure of neurons in the hippocampal CA3 region were examined. The thermal pain threshold (paw withdrawal latency) was increased significantly in both groups at 2 weeks after electroacupuncture intervention compared with 2 days of electroacupuncture. In ovariectomized rats with chronic constriction injury, the analgesic effect was significantly reduced. Electroacupuncture for 2 weeks significantly diminished the injury-induced increase in synaptic cleft width and thinning of the postsynaptic density, and it significantly suppressed the down-regulation of intracellular calcium/calmodulin-dependent protein kinase II expression in the hippocampal CA3 region. Repeated electroacupuncture intervention had a cumulative analgesic effect on injury-induced neuropathic pain reactions, and it led to synaptic remodeling of hippocampal neurons and upregulated calcium/calmodulin-dependent protein kinase II expression in the hippocampal CA3 region.

No MeSH data available.


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Comparison of calcium/calmodulin-dependent kinase II expression (integrated grey value) in hippocampal CA3 region among the various groups.Data were expressed as mean ± SD (n = 4) and analyzed by one-way analysis of variance and least significant difference-t test.aP < 0.05, vs. normal control group; bP < 0.05, vs. CCI group; cP < 0.05, vs. CCI + EA2D group; dP < 0.05, vs. OVX + CCI group; eP < 0.05, vs. OVX + CCI + EA2D group; fP < 0.05, vs. CCI + EA2W group.CCI: Chronic constrictive injury; OVX: ovariectomy; EA: electroacupuncture; 2D: 2 days; 2W: 2 weeks.
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Figure 4: Comparison of calcium/calmodulin-dependent kinase II expression (integrated grey value) in hippocampal CA3 region among the various groups.Data were expressed as mean ± SD (n = 4) and analyzed by one-way analysis of variance and least significant difference-t test.aP < 0.05, vs. normal control group; bP < 0.05, vs. CCI group; cP < 0.05, vs. CCI + EA2D group; dP < 0.05, vs. OVX + CCI group; eP < 0.05, vs. OVX + CCI + EA2D group; fP < 0.05, vs. CCI + EA2W group.CCI: Chronic constrictive injury; OVX: ovariectomy; EA: electroacupuncture; 2D: 2 days; 2W: 2 weeks.

Mentions: CaMKII is an important calcium messenger regulating hippocampal synaptic plasticity and learning. The integral grey values of CaMKII expression were significantly increased in the CCI and OVX + CCI groups compared with the normal control group (P < 0.05), a downregulation of this protein in CCI and OVX + CCI rats. Compared with the CCI group, the integral grey value was significantly lower in the CCI + electroacupuncture 2 weeks group compared with the CCI group (P < 0.05), but remained unchanged in the CCI + electroacupuncture 2 days group. The change in CaMKII expression in CCI rats was similar to that in OVX + CCI rats. The integral grey value of CaMKII immunoreactivity was significantly higher in the OVX + CCI + electroacupuncture 2 weeks group compared with the CCI + electroacupuncture 2 weeks group (P < 0.05). Collectively, these results indicate that electroacupuncture upregulates CaMKII expression in the hippocampal CA3 region (Figure 4).


The cumulative analgesic effect of repeated electroacupuncture involves synaptic remodeling in the hippocampal CA3 region.

Xu Q, Liu T, Chen S, Gao Y, Wang J, Qiao L, Liu J - Neural Regen Res (2012)

Comparison of calcium/calmodulin-dependent kinase II expression (integrated grey value) in hippocampal CA3 region among the various groups.Data were expressed as mean ± SD (n = 4) and analyzed by one-way analysis of variance and least significant difference-t test.aP < 0.05, vs. normal control group; bP < 0.05, vs. CCI group; cP < 0.05, vs. CCI + EA2D group; dP < 0.05, vs. OVX + CCI group; eP < 0.05, vs. OVX + CCI + EA2D group; fP < 0.05, vs. CCI + EA2W group.CCI: Chronic constrictive injury; OVX: ovariectomy; EA: electroacupuncture; 2D: 2 days; 2W: 2 weeks.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308787&req=5

Figure 4: Comparison of calcium/calmodulin-dependent kinase II expression (integrated grey value) in hippocampal CA3 region among the various groups.Data were expressed as mean ± SD (n = 4) and analyzed by one-way analysis of variance and least significant difference-t test.aP < 0.05, vs. normal control group; bP < 0.05, vs. CCI group; cP < 0.05, vs. CCI + EA2D group; dP < 0.05, vs. OVX + CCI group; eP < 0.05, vs. OVX + CCI + EA2D group; fP < 0.05, vs. CCI + EA2W group.CCI: Chronic constrictive injury; OVX: ovariectomy; EA: electroacupuncture; 2D: 2 days; 2W: 2 weeks.
Mentions: CaMKII is an important calcium messenger regulating hippocampal synaptic plasticity and learning. The integral grey values of CaMKII expression were significantly increased in the CCI and OVX + CCI groups compared with the normal control group (P < 0.05), a downregulation of this protein in CCI and OVX + CCI rats. Compared with the CCI group, the integral grey value was significantly lower in the CCI + electroacupuncture 2 weeks group compared with the CCI group (P < 0.05), but remained unchanged in the CCI + electroacupuncture 2 days group. The change in CaMKII expression in CCI rats was similar to that in OVX + CCI rats. The integral grey value of CaMKII immunoreactivity was significantly higher in the OVX + CCI + electroacupuncture 2 weeks group compared with the CCI + electroacupuncture 2 weeks group (P < 0.05). Collectively, these results indicate that electroacupuncture upregulates CaMKII expression in the hippocampal CA3 region (Figure 4).

Bottom Line: In addition, concomitant changes in calcium/calmodulin-dependent protein kinase II expression and synaptic ultrastructure of neurons in the hippocampal CA3 region were examined.In ovariectomized rats with chronic constriction injury, the analgesic effect was significantly reduced.Repeated electroacupuncture intervention had a cumulative analgesic effect on injury-induced neuropathic pain reactions, and it led to synaptic remodeling of hippocampal neurons and upregulated calcium/calmodulin-dependent protein kinase II expression in the hippocampal CA3 region.

View Article: PubMed Central - PubMed

Affiliation: Hainan Medical University, Haikou 571101, Hainan Province, China.

ABSTRACT
In the present study, we examined the analgesic effect of repeated electroacupuncture at bilateral Zusanli (ST36) and Yanglingquan (GB34) once a day for 14 consecutive days in a rat model of chronic sciatic nerve constriction injury-induced neuropathic pain. In addition, concomitant changes in calcium/calmodulin-dependent protein kinase II expression and synaptic ultrastructure of neurons in the hippocampal CA3 region were examined. The thermal pain threshold (paw withdrawal latency) was increased significantly in both groups at 2 weeks after electroacupuncture intervention compared with 2 days of electroacupuncture. In ovariectomized rats with chronic constriction injury, the analgesic effect was significantly reduced. Electroacupuncture for 2 weeks significantly diminished the injury-induced increase in synaptic cleft width and thinning of the postsynaptic density, and it significantly suppressed the down-regulation of intracellular calcium/calmodulin-dependent protein kinase II expression in the hippocampal CA3 region. Repeated electroacupuncture intervention had a cumulative analgesic effect on injury-induced neuropathic pain reactions, and it led to synaptic remodeling of hippocampal neurons and upregulated calcium/calmodulin-dependent protein kinase II expression in the hippocampal CA3 region.

No MeSH data available.


Related in: MedlinePlus