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The cumulative analgesic effect of repeated electroacupuncture involves synaptic remodeling in the hippocampal CA3 region.

Xu Q, Liu T, Chen S, Gao Y, Wang J, Qiao L, Liu J - Neural Regen Res (2012)

Bottom Line: In addition, concomitant changes in calcium/calmodulin-dependent protein kinase II expression and synaptic ultrastructure of neurons in the hippocampal CA3 region were examined.In ovariectomized rats with chronic constriction injury, the analgesic effect was significantly reduced.Repeated electroacupuncture intervention had a cumulative analgesic effect on injury-induced neuropathic pain reactions, and it led to synaptic remodeling of hippocampal neurons and upregulated calcium/calmodulin-dependent protein kinase II expression in the hippocampal CA3 region.

View Article: PubMed Central - PubMed

Affiliation: Hainan Medical University, Haikou 571101, Hainan Province, China.

ABSTRACT
In the present study, we examined the analgesic effect of repeated electroacupuncture at bilateral Zusanli (ST36) and Yanglingquan (GB34) once a day for 14 consecutive days in a rat model of chronic sciatic nerve constriction injury-induced neuropathic pain. In addition, concomitant changes in calcium/calmodulin-dependent protein kinase II expression and synaptic ultrastructure of neurons in the hippocampal CA3 region were examined. The thermal pain threshold (paw withdrawal latency) was increased significantly in both groups at 2 weeks after electroacupuncture intervention compared with 2 days of electroacupuncture. In ovariectomized rats with chronic constriction injury, the analgesic effect was significantly reduced. Electroacupuncture for 2 weeks significantly diminished the injury-induced increase in synaptic cleft width and thinning of the postsynaptic density, and it significantly suppressed the down-regulation of intracellular calcium/calmodulin-dependent protein kinase II expression in the hippocampal CA3 region. Repeated electroacupuncture intervention had a cumulative analgesic effect on injury-induced neuropathic pain reactions, and it led to synaptic remodeling of hippocampal neurons and upregulated calcium/calmodulin-dependent protein kinase II expression in the hippocampal CA3 region.

No MeSH data available.


Related in: MedlinePlus

Comparison of synaptic active zone values of neurons in the hippocampal CA3 region among the various groups.Data were expressed as mean ± SD (n = 4) and analyzed by one-way analysis of variance and least significant difference-t test.aP < 0.05, vs. normal control group; bP < 0.05, vs. CCI group; cP < 0.05, vs. CCI + EA2D group; dP < 0.05, vs. OVX + CCI group; eP < 0.05, vs. OVX + CCI + EA2D group.CCI: Chronic constrictive injury; OVX: ovariectomy; EA: electroacupuncture; 2D: 2 days; 2W: 2 weeks.
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Figure 3: Comparison of synaptic active zone values of neurons in the hippocampal CA3 region among the various groups.Data were expressed as mean ± SD (n = 4) and analyzed by one-way analysis of variance and least significant difference-t test.aP < 0.05, vs. normal control group; bP < 0.05, vs. CCI group; cP < 0.05, vs. CCI + EA2D group; dP < 0.05, vs. OVX + CCI group; eP < 0.05, vs. OVX + CCI + EA2D group.CCI: Chronic constrictive injury; OVX: ovariectomy; EA: electroacupuncture; 2D: 2 days; 2W: 2 weeks.

Mentions: Compared with the control group, the active zone length values for the CCI and OVX + CCI groups were significantly decreased (P < 0.05). The synaptic active zone length was significantly longer in the CCI + electroacupuncture 2 weeks group compared with the CCI group, and in the OVX + CCI + electroacupuncture 2 weeks group compared with the OVX + CCI group (both P < 0.05), suggesting an improvement of synaptic efficacy after repeated electroacupuncture. The active zone length was significantly greater in the CCI + electroacupuncture 2 weeks group compared with the OVX + CCI + electroacupuncture 2 weeks group (P < 0.05). No significant differences were found between the CCI and CCI + electroacupuncture 2 days groups or between the OVX + CCI and OVX + CCI + electroacupuncture 2 days groups in active zone length (both P > 0.05; Figure 3).


The cumulative analgesic effect of repeated electroacupuncture involves synaptic remodeling in the hippocampal CA3 region.

Xu Q, Liu T, Chen S, Gao Y, Wang J, Qiao L, Liu J - Neural Regen Res (2012)

Comparison of synaptic active zone values of neurons in the hippocampal CA3 region among the various groups.Data were expressed as mean ± SD (n = 4) and analyzed by one-way analysis of variance and least significant difference-t test.aP < 0.05, vs. normal control group; bP < 0.05, vs. CCI group; cP < 0.05, vs. CCI + EA2D group; dP < 0.05, vs. OVX + CCI group; eP < 0.05, vs. OVX + CCI + EA2D group.CCI: Chronic constrictive injury; OVX: ovariectomy; EA: electroacupuncture; 2D: 2 days; 2W: 2 weeks.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308787&req=5

Figure 3: Comparison of synaptic active zone values of neurons in the hippocampal CA3 region among the various groups.Data were expressed as mean ± SD (n = 4) and analyzed by one-way analysis of variance and least significant difference-t test.aP < 0.05, vs. normal control group; bP < 0.05, vs. CCI group; cP < 0.05, vs. CCI + EA2D group; dP < 0.05, vs. OVX + CCI group; eP < 0.05, vs. OVX + CCI + EA2D group.CCI: Chronic constrictive injury; OVX: ovariectomy; EA: electroacupuncture; 2D: 2 days; 2W: 2 weeks.
Mentions: Compared with the control group, the active zone length values for the CCI and OVX + CCI groups were significantly decreased (P < 0.05). The synaptic active zone length was significantly longer in the CCI + electroacupuncture 2 weeks group compared with the CCI group, and in the OVX + CCI + electroacupuncture 2 weeks group compared with the OVX + CCI group (both P < 0.05), suggesting an improvement of synaptic efficacy after repeated electroacupuncture. The active zone length was significantly greater in the CCI + electroacupuncture 2 weeks group compared with the OVX + CCI + electroacupuncture 2 weeks group (P < 0.05). No significant differences were found between the CCI and CCI + electroacupuncture 2 days groups or between the OVX + CCI and OVX + CCI + electroacupuncture 2 days groups in active zone length (both P > 0.05; Figure 3).

Bottom Line: In addition, concomitant changes in calcium/calmodulin-dependent protein kinase II expression and synaptic ultrastructure of neurons in the hippocampal CA3 region were examined.In ovariectomized rats with chronic constriction injury, the analgesic effect was significantly reduced.Repeated electroacupuncture intervention had a cumulative analgesic effect on injury-induced neuropathic pain reactions, and it led to synaptic remodeling of hippocampal neurons and upregulated calcium/calmodulin-dependent protein kinase II expression in the hippocampal CA3 region.

View Article: PubMed Central - PubMed

Affiliation: Hainan Medical University, Haikou 571101, Hainan Province, China.

ABSTRACT
In the present study, we examined the analgesic effect of repeated electroacupuncture at bilateral Zusanli (ST36) and Yanglingquan (GB34) once a day for 14 consecutive days in a rat model of chronic sciatic nerve constriction injury-induced neuropathic pain. In addition, concomitant changes in calcium/calmodulin-dependent protein kinase II expression and synaptic ultrastructure of neurons in the hippocampal CA3 region were examined. The thermal pain threshold (paw withdrawal latency) was increased significantly in both groups at 2 weeks after electroacupuncture intervention compared with 2 days of electroacupuncture. In ovariectomized rats with chronic constriction injury, the analgesic effect was significantly reduced. Electroacupuncture for 2 weeks significantly diminished the injury-induced increase in synaptic cleft width and thinning of the postsynaptic density, and it significantly suppressed the down-regulation of intracellular calcium/calmodulin-dependent protein kinase II expression in the hippocampal CA3 region. Repeated electroacupuncture intervention had a cumulative analgesic effect on injury-induced neuropathic pain reactions, and it led to synaptic remodeling of hippocampal neurons and upregulated calcium/calmodulin-dependent protein kinase II expression in the hippocampal CA3 region.

No MeSH data available.


Related in: MedlinePlus