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Mitogen activated protein kinase signaling pathways participate in the active principle region of Buyang Huanwu decoction-induced differentiation of bone marrow mesenchymal stem cells.

Zheng J, Liang J, Deng X, Chen X, Wu F, Zhao X, Luo Y, Fu L, Jiang Z - Neural Regen Res (2012)

Bottom Line: Mitogen-activated protein kinase signaling was identified as one of the key pathways underlying this differentiation process.Cellular signaling pathway blocking agents, PD98059 and SB203580, inhibited extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways respectively. mRNA and protein expression of the neuronal marker, neuron specific enolase, and neural stem cell marker, nestin, were decreased in bone marrow mesenchymal stem cells after treatment with the active principle region of Buyang Huanwu decoction.Experimental findings indicate that, extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways participate in bone marrow mesenchymal stem cell differentiation into neuron-like cells, induced by the active principle region of Buyang Huanwu decoction.

View Article: PubMed Central - PubMed

Affiliation: Ruikang Hospital Attached to Guangxi Traditional Chinese Medicine College, Level-3 Laboratory of Cell Molecular Biology of State Administration of Traditional Chinese Medicine of the People's Republic of China, Nanning 530011, Guangxi Zhuang Autonomous Region, China.

ABSTRACT
Our preliminary studies confirmed that an active principle region of Buyang Huanwu decoction, comprising alkaloid, polysaccharide, aglycon, glucoside and volatile oil, can induce bone marrow mesenchymal stem cell differentiation into neurons. Mitogen-activated protein kinase signaling was identified as one of the key pathways underlying this differentiation process. The present study shows phosphorylated extracellular signal-regulated protein kinase and phosphorylated p38 protein expression was increased after differentiation. Cellular signaling pathway blocking agents, PD98059 and SB203580, inhibited extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways respectively. mRNA and protein expression of the neuronal marker, neuron specific enolase, and neural stem cell marker, nestin, were decreased in bone marrow mesenchymal stem cells after treatment with the active principle region of Buyang Huanwu decoction. Experimental findings indicate that, extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways participate in bone marrow mesenchymal stem cell differentiation into neuron-like cells, induced by the active principle region of Buyang Huanwu decoction.

No MeSH data available.


Related in: MedlinePlus

Western blot electropherogram of nestin protein expression after the mitogen-activated protein kinase pathway was blocked.1: apr-BYHWD group; 2: apr-BYHWD + SB203580 group; 3: apr-BYHWD + PD98059 group. BYHWD: Buyang Huanwu decoction; apr-BYHWD: active principle region of BYHWD; PD98059 and SB203580: cellular signaling pathway blocking agents.
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Figure 8: Western blot electropherogram of nestin protein expression after the mitogen-activated protein kinase pathway was blocked.1: apr-BYHWD group; 2: apr-BYHWD + SB203580 group; 3: apr-BYHWD + PD98059 group. BYHWD: Buyang Huanwu decoction; apr-BYHWD: active principle region of BYHWD; PD98059 and SB203580: cellular signaling pathway blocking agents.

Mentions: Western blot analysis was used to detect nestin protein expression in BMSCs after the MAPK pathway was blocked. Expression was absent in both the apr-BYHWD + SB203580 and apr-BYHWD + PD98059 groups at days 7 and 14. Low level nestin protein expression was detected at day 28 in both these groups, but was significantly lower compared to the apr-BYHWD group (P < 0.05; Figure 8, Table 3).


Mitogen activated protein kinase signaling pathways participate in the active principle region of Buyang Huanwu decoction-induced differentiation of bone marrow mesenchymal stem cells.

Zheng J, Liang J, Deng X, Chen X, Wu F, Zhao X, Luo Y, Fu L, Jiang Z - Neural Regen Res (2012)

Western blot electropherogram of nestin protein expression after the mitogen-activated protein kinase pathway was blocked.1: apr-BYHWD group; 2: apr-BYHWD + SB203580 group; 3: apr-BYHWD + PD98059 group. BYHWD: Buyang Huanwu decoction; apr-BYHWD: active principle region of BYHWD; PD98059 and SB203580: cellular signaling pathway blocking agents.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308786&req=5

Figure 8: Western blot electropherogram of nestin protein expression after the mitogen-activated protein kinase pathway was blocked.1: apr-BYHWD group; 2: apr-BYHWD + SB203580 group; 3: apr-BYHWD + PD98059 group. BYHWD: Buyang Huanwu decoction; apr-BYHWD: active principle region of BYHWD; PD98059 and SB203580: cellular signaling pathway blocking agents.
Mentions: Western blot analysis was used to detect nestin protein expression in BMSCs after the MAPK pathway was blocked. Expression was absent in both the apr-BYHWD + SB203580 and apr-BYHWD + PD98059 groups at days 7 and 14. Low level nestin protein expression was detected at day 28 in both these groups, but was significantly lower compared to the apr-BYHWD group (P < 0.05; Figure 8, Table 3).

Bottom Line: Mitogen-activated protein kinase signaling was identified as one of the key pathways underlying this differentiation process.Cellular signaling pathway blocking agents, PD98059 and SB203580, inhibited extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways respectively. mRNA and protein expression of the neuronal marker, neuron specific enolase, and neural stem cell marker, nestin, were decreased in bone marrow mesenchymal stem cells after treatment with the active principle region of Buyang Huanwu decoction.Experimental findings indicate that, extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways participate in bone marrow mesenchymal stem cell differentiation into neuron-like cells, induced by the active principle region of Buyang Huanwu decoction.

View Article: PubMed Central - PubMed

Affiliation: Ruikang Hospital Attached to Guangxi Traditional Chinese Medicine College, Level-3 Laboratory of Cell Molecular Biology of State Administration of Traditional Chinese Medicine of the People's Republic of China, Nanning 530011, Guangxi Zhuang Autonomous Region, China.

ABSTRACT
Our preliminary studies confirmed that an active principle region of Buyang Huanwu decoction, comprising alkaloid, polysaccharide, aglycon, glucoside and volatile oil, can induce bone marrow mesenchymal stem cell differentiation into neurons. Mitogen-activated protein kinase signaling was identified as one of the key pathways underlying this differentiation process. The present study shows phosphorylated extracellular signal-regulated protein kinase and phosphorylated p38 protein expression was increased after differentiation. Cellular signaling pathway blocking agents, PD98059 and SB203580, inhibited extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways respectively. mRNA and protein expression of the neuronal marker, neuron specific enolase, and neural stem cell marker, nestin, were decreased in bone marrow mesenchymal stem cells after treatment with the active principle region of Buyang Huanwu decoction. Experimental findings indicate that, extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways participate in bone marrow mesenchymal stem cell differentiation into neuron-like cells, induced by the active principle region of Buyang Huanwu decoction.

No MeSH data available.


Related in: MedlinePlus