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Mitogen activated protein kinase signaling pathways participate in the active principle region of Buyang Huanwu decoction-induced differentiation of bone marrow mesenchymal stem cells.

Zheng J, Liang J, Deng X, Chen X, Wu F, Zhao X, Luo Y, Fu L, Jiang Z - Neural Regen Res (2012)

Bottom Line: Mitogen-activated protein kinase signaling was identified as one of the key pathways underlying this differentiation process.Cellular signaling pathway blocking agents, PD98059 and SB203580, inhibited extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways respectively. mRNA and protein expression of the neuronal marker, neuron specific enolase, and neural stem cell marker, nestin, were decreased in bone marrow mesenchymal stem cells after treatment with the active principle region of Buyang Huanwu decoction.Experimental findings indicate that, extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways participate in bone marrow mesenchymal stem cell differentiation into neuron-like cells, induced by the active principle region of Buyang Huanwu decoction.

View Article: PubMed Central - PubMed

Affiliation: Ruikang Hospital Attached to Guangxi Traditional Chinese Medicine College, Level-3 Laboratory of Cell Molecular Biology of State Administration of Traditional Chinese Medicine of the People's Republic of China, Nanning 530011, Guangxi Zhuang Autonomous Region, China.

ABSTRACT
Our preliminary studies confirmed that an active principle region of Buyang Huanwu decoction, comprising alkaloid, polysaccharide, aglycon, glucoside and volatile oil, can induce bone marrow mesenchymal stem cell differentiation into neurons. Mitogen-activated protein kinase signaling was identified as one of the key pathways underlying this differentiation process. The present study shows phosphorylated extracellular signal-regulated protein kinase and phosphorylated p38 protein expression was increased after differentiation. Cellular signaling pathway blocking agents, PD98059 and SB203580, inhibited extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways respectively. mRNA and protein expression of the neuronal marker, neuron specific enolase, and neural stem cell marker, nestin, were decreased in bone marrow mesenchymal stem cells after treatment with the active principle region of Buyang Huanwu decoction. Experimental findings indicate that, extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways participate in bone marrow mesenchymal stem cell differentiation into neuron-like cells, induced by the active principle region of Buyang Huanwu decoction.

No MeSH data available.


Related in: MedlinePlus

Reverse transcription-PCR electropherogram of neuron specific enolase mRNA expression after the mitogen-activated protein kinase pathway was blocked.1: apr-BYHWD group; 2: apr-BYHWD + SB203580 group; 3: apr-BYHWD + PD98059 group; M: Marker; BYHWD: Buyang Huanwu decoction; apr-BYHWD: active principle region of BYHWD; PD98059 and SB203580: cellular signaling pathway blocking agents.
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Figure 5: Reverse transcription-PCR electropherogram of neuron specific enolase mRNA expression after the mitogen-activated protein kinase pathway was blocked.1: apr-BYHWD group; 2: apr-BYHWD + SB203580 group; 3: apr-BYHWD + PD98059 group; M: Marker; BYHWD: Buyang Huanwu decoction; apr-BYHWD: active principle region of BYHWD; PD98059 and SB203580: cellular signaling pathway blocking agents.

Mentions: Reverse transcription-PCR (RT-PCR) confirmed NSE mRNA expression in the apr-BYHWD control group at days 7, 14 and 28. NSE mRNA expression in the apr-BYHWD + SB203580 group was significantly lower than those in the apr-BYHWD group at days 14 and 28 (P < 0.05; Figure 5, Table 2). At days 7, 14 and 28, NSE mRNA expression levels in the apr-BYHWD + PD98059 group were significantly lower than those in the apr-BYHWD group (P < 0.05; Figure 5, Table 2). Western blot analysis was used to detect expression of NSE protein in BMSCs after MAPK pathways were blocked. The results showed that NSE protein expression levels in the apr-BYHWD + SB203580 and apr-BYHWD + PD98059 groups, were lower than those in the apr-BYHWD group at days 7, 14 and 28 (P < 0.05; Figure 6, Table 2).


Mitogen activated protein kinase signaling pathways participate in the active principle region of Buyang Huanwu decoction-induced differentiation of bone marrow mesenchymal stem cells.

Zheng J, Liang J, Deng X, Chen X, Wu F, Zhao X, Luo Y, Fu L, Jiang Z - Neural Regen Res (2012)

Reverse transcription-PCR electropherogram of neuron specific enolase mRNA expression after the mitogen-activated protein kinase pathway was blocked.1: apr-BYHWD group; 2: apr-BYHWD + SB203580 group; 3: apr-BYHWD + PD98059 group; M: Marker; BYHWD: Buyang Huanwu decoction; apr-BYHWD: active principle region of BYHWD; PD98059 and SB203580: cellular signaling pathway blocking agents.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308786&req=5

Figure 5: Reverse transcription-PCR electropherogram of neuron specific enolase mRNA expression after the mitogen-activated protein kinase pathway was blocked.1: apr-BYHWD group; 2: apr-BYHWD + SB203580 group; 3: apr-BYHWD + PD98059 group; M: Marker; BYHWD: Buyang Huanwu decoction; apr-BYHWD: active principle region of BYHWD; PD98059 and SB203580: cellular signaling pathway blocking agents.
Mentions: Reverse transcription-PCR (RT-PCR) confirmed NSE mRNA expression in the apr-BYHWD control group at days 7, 14 and 28. NSE mRNA expression in the apr-BYHWD + SB203580 group was significantly lower than those in the apr-BYHWD group at days 14 and 28 (P < 0.05; Figure 5, Table 2). At days 7, 14 and 28, NSE mRNA expression levels in the apr-BYHWD + PD98059 group were significantly lower than those in the apr-BYHWD group (P < 0.05; Figure 5, Table 2). Western blot analysis was used to detect expression of NSE protein in BMSCs after MAPK pathways were blocked. The results showed that NSE protein expression levels in the apr-BYHWD + SB203580 and apr-BYHWD + PD98059 groups, were lower than those in the apr-BYHWD group at days 7, 14 and 28 (P < 0.05; Figure 6, Table 2).

Bottom Line: Mitogen-activated protein kinase signaling was identified as one of the key pathways underlying this differentiation process.Cellular signaling pathway blocking agents, PD98059 and SB203580, inhibited extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways respectively. mRNA and protein expression of the neuronal marker, neuron specific enolase, and neural stem cell marker, nestin, were decreased in bone marrow mesenchymal stem cells after treatment with the active principle region of Buyang Huanwu decoction.Experimental findings indicate that, extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways participate in bone marrow mesenchymal stem cell differentiation into neuron-like cells, induced by the active principle region of Buyang Huanwu decoction.

View Article: PubMed Central - PubMed

Affiliation: Ruikang Hospital Attached to Guangxi Traditional Chinese Medicine College, Level-3 Laboratory of Cell Molecular Biology of State Administration of Traditional Chinese Medicine of the People's Republic of China, Nanning 530011, Guangxi Zhuang Autonomous Region, China.

ABSTRACT
Our preliminary studies confirmed that an active principle region of Buyang Huanwu decoction, comprising alkaloid, polysaccharide, aglycon, glucoside and volatile oil, can induce bone marrow mesenchymal stem cell differentiation into neurons. Mitogen-activated protein kinase signaling was identified as one of the key pathways underlying this differentiation process. The present study shows phosphorylated extracellular signal-regulated protein kinase and phosphorylated p38 protein expression was increased after differentiation. Cellular signaling pathway blocking agents, PD98059 and SB203580, inhibited extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways respectively. mRNA and protein expression of the neuronal marker, neuron specific enolase, and neural stem cell marker, nestin, were decreased in bone marrow mesenchymal stem cells after treatment with the active principle region of Buyang Huanwu decoction. Experimental findings indicate that, extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways participate in bone marrow mesenchymal stem cell differentiation into neuron-like cells, induced by the active principle region of Buyang Huanwu decoction.

No MeSH data available.


Related in: MedlinePlus