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Mitogen activated protein kinase signaling pathways participate in the active principle region of Buyang Huanwu decoction-induced differentiation of bone marrow mesenchymal stem cells.

Zheng J, Liang J, Deng X, Chen X, Wu F, Zhao X, Luo Y, Fu L, Jiang Z - Neural Regen Res (2012)

Bottom Line: Mitogen-activated protein kinase signaling was identified as one of the key pathways underlying this differentiation process.The present study shows phosphorylated extracellular signal-regulated protein kinase and phosphorylated p38 protein expression was increased after differentiation.Cellular signaling pathway blocking agents, PD98059 and SB203580, inhibited extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways respectively. mRNA and protein expression of the neuronal marker, neuron specific enolase, and neural stem cell marker, nestin, were decreased in bone marrow mesenchymal stem cells after treatment with the active principle region of Buyang Huanwu decoction.

View Article: PubMed Central - PubMed

Affiliation: Ruikang Hospital Attached to Guangxi Traditional Chinese Medicine College, Level-3 Laboratory of Cell Molecular Biology of State Administration of Traditional Chinese Medicine of the People's Republic of China, Nanning 530011, Guangxi Zhuang Autonomous Region, China.

ABSTRACT
Our preliminary studies confirmed that an active principle region of Buyang Huanwu decoction, comprising alkaloid, polysaccharide, aglycon, glucoside and volatile oil, can induce bone marrow mesenchymal stem cell differentiation into neurons. Mitogen-activated protein kinase signaling was identified as one of the key pathways underlying this differentiation process. The present study shows phosphorylated extracellular signal-regulated protein kinase and phosphorylated p38 protein expression was increased after differentiation. Cellular signaling pathway blocking agents, PD98059 and SB203580, inhibited extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways respectively. mRNA and protein expression of the neuronal marker, neuron specific enolase, and neural stem cell marker, nestin, were decreased in bone marrow mesenchymal stem cells after treatment with the active principle region of Buyang Huanwu decoction. Experimental findings indicate that, extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways participate in bone marrow mesenchymal stem cell differentiation into neuron-like cells, induced by the active principle region of Buyang Huanwu decoction.

No MeSH data available.


Related in: MedlinePlus

Electropherogram of p38 phosphorylated protein (p-p38) expression in rat bone marrow mesenchymal stem cells induced with Buyang Huanwu decoction (BYHWD) detected by western blot analysis.1: Blank control group; 2: negative control group; 3: apr-BYHWD group. In the apr-BYHWD group, p38 phosphorylated protein expression peaked at day 28. apr-BYHWD: Active principle region of BYHWD.
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Figure 4: Electropherogram of p38 phosphorylated protein (p-p38) expression in rat bone marrow mesenchymal stem cells induced with Buyang Huanwu decoction (BYHWD) detected by western blot analysis.1: Blank control group; 2: negative control group; 3: apr-BYHWD group. In the apr-BYHWD group, p38 phosphorylated protein expression peaked at day 28. apr-BYHWD: Active principle region of BYHWD.

Mentions: Cells in the apr-BYHWD group were induced for 28 days. ERK protein levels were significantly higher in the apr-BYHWD group at day 28 compared with the blank control and negative control groups (P < 0.05; Table 1, Figure 3). At days 7, 14 and 28, p38 phosphorylated protein levels were significantly increased in the apr-BYHWD group compared with the blank control and negative control groups (P < 0.05; Table 1, Figure 4).


Mitogen activated protein kinase signaling pathways participate in the active principle region of Buyang Huanwu decoction-induced differentiation of bone marrow mesenchymal stem cells.

Zheng J, Liang J, Deng X, Chen X, Wu F, Zhao X, Luo Y, Fu L, Jiang Z - Neural Regen Res (2012)

Electropherogram of p38 phosphorylated protein (p-p38) expression in rat bone marrow mesenchymal stem cells induced with Buyang Huanwu decoction (BYHWD) detected by western blot analysis.1: Blank control group; 2: negative control group; 3: apr-BYHWD group. In the apr-BYHWD group, p38 phosphorylated protein expression peaked at day 28. apr-BYHWD: Active principle region of BYHWD.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308786&req=5

Figure 4: Electropherogram of p38 phosphorylated protein (p-p38) expression in rat bone marrow mesenchymal stem cells induced with Buyang Huanwu decoction (BYHWD) detected by western blot analysis.1: Blank control group; 2: negative control group; 3: apr-BYHWD group. In the apr-BYHWD group, p38 phosphorylated protein expression peaked at day 28. apr-BYHWD: Active principle region of BYHWD.
Mentions: Cells in the apr-BYHWD group were induced for 28 days. ERK protein levels were significantly higher in the apr-BYHWD group at day 28 compared with the blank control and negative control groups (P < 0.05; Table 1, Figure 3). At days 7, 14 and 28, p38 phosphorylated protein levels were significantly increased in the apr-BYHWD group compared with the blank control and negative control groups (P < 0.05; Table 1, Figure 4).

Bottom Line: Mitogen-activated protein kinase signaling was identified as one of the key pathways underlying this differentiation process.The present study shows phosphorylated extracellular signal-regulated protein kinase and phosphorylated p38 protein expression was increased after differentiation.Cellular signaling pathway blocking agents, PD98059 and SB203580, inhibited extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways respectively. mRNA and protein expression of the neuronal marker, neuron specific enolase, and neural stem cell marker, nestin, were decreased in bone marrow mesenchymal stem cells after treatment with the active principle region of Buyang Huanwu decoction.

View Article: PubMed Central - PubMed

Affiliation: Ruikang Hospital Attached to Guangxi Traditional Chinese Medicine College, Level-3 Laboratory of Cell Molecular Biology of State Administration of Traditional Chinese Medicine of the People's Republic of China, Nanning 530011, Guangxi Zhuang Autonomous Region, China.

ABSTRACT
Our preliminary studies confirmed that an active principle region of Buyang Huanwu decoction, comprising alkaloid, polysaccharide, aglycon, glucoside and volatile oil, can induce bone marrow mesenchymal stem cell differentiation into neurons. Mitogen-activated protein kinase signaling was identified as one of the key pathways underlying this differentiation process. The present study shows phosphorylated extracellular signal-regulated protein kinase and phosphorylated p38 protein expression was increased after differentiation. Cellular signaling pathway blocking agents, PD98059 and SB203580, inhibited extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways respectively. mRNA and protein expression of the neuronal marker, neuron specific enolase, and neural stem cell marker, nestin, were decreased in bone marrow mesenchymal stem cells after treatment with the active principle region of Buyang Huanwu decoction. Experimental findings indicate that, extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways participate in bone marrow mesenchymal stem cell differentiation into neuron-like cells, induced by the active principle region of Buyang Huanwu decoction.

No MeSH data available.


Related in: MedlinePlus