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Mitogen activated protein kinase signaling pathways participate in the active principle region of Buyang Huanwu decoction-induced differentiation of bone marrow mesenchymal stem cells.

Zheng J, Liang J, Deng X, Chen X, Wu F, Zhao X, Luo Y, Fu L, Jiang Z - Neural Regen Res (2012)

Bottom Line: Mitogen-activated protein kinase signaling was identified as one of the key pathways underlying this differentiation process.The present study shows phosphorylated extracellular signal-regulated protein kinase and phosphorylated p38 protein expression was increased after differentiation.Cellular signaling pathway blocking agents, PD98059 and SB203580, inhibited extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways respectively. mRNA and protein expression of the neuronal marker, neuron specific enolase, and neural stem cell marker, nestin, were decreased in bone marrow mesenchymal stem cells after treatment with the active principle region of Buyang Huanwu decoction.

View Article: PubMed Central - PubMed

Affiliation: Ruikang Hospital Attached to Guangxi Traditional Chinese Medicine College, Level-3 Laboratory of Cell Molecular Biology of State Administration of Traditional Chinese Medicine of the People's Republic of China, Nanning 530011, Guangxi Zhuang Autonomous Region, China.

ABSTRACT
Our preliminary studies confirmed that an active principle region of Buyang Huanwu decoction, comprising alkaloid, polysaccharide, aglycon, glucoside and volatile oil, can induce bone marrow mesenchymal stem cell differentiation into neurons. Mitogen-activated protein kinase signaling was identified as one of the key pathways underlying this differentiation process. The present study shows phosphorylated extracellular signal-regulated protein kinase and phosphorylated p38 protein expression was increased after differentiation. Cellular signaling pathway blocking agents, PD98059 and SB203580, inhibited extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways respectively. mRNA and protein expression of the neuronal marker, neuron specific enolase, and neural stem cell marker, nestin, were decreased in bone marrow mesenchymal stem cells after treatment with the active principle region of Buyang Huanwu decoction. Experimental findings indicate that, extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways participate in bone marrow mesenchymal stem cell differentiation into neuron-like cells, induced by the active principle region of Buyang Huanwu decoction.

No MeSH data available.


Related in: MedlinePlus

CD11b, CD45 and CD90 expression in rat bone marrow mesenchymal stem cells detected by flow cytometry.(A) Blank control cells. (B-D) FITC labeled CD90, CD11 and CD45 positive cells respectively. FITC: Fluorescein isothiocyanate.
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Figure 2: CD11b, CD45 and CD90 expression in rat bone marrow mesenchymal stem cells detected by flow cytometry.(A) Blank control cells. (B-D) FITC labeled CD90, CD11 and CD45 positive cells respectively. FITC: Fluorescein isothiocyanate.

Mentions: Flow cytometry was used to determine the cell surface antigen profile of BMSCs. Analysis showed expression of CD90 in more than 98% of BMSCs, while expression levels of CD11b and CD45 were less than 2% (Figure 2). This indicated the BMSC population has good uniformity and high purity.


Mitogen activated protein kinase signaling pathways participate in the active principle region of Buyang Huanwu decoction-induced differentiation of bone marrow mesenchymal stem cells.

Zheng J, Liang J, Deng X, Chen X, Wu F, Zhao X, Luo Y, Fu L, Jiang Z - Neural Regen Res (2012)

CD11b, CD45 and CD90 expression in rat bone marrow mesenchymal stem cells detected by flow cytometry.(A) Blank control cells. (B-D) FITC labeled CD90, CD11 and CD45 positive cells respectively. FITC: Fluorescein isothiocyanate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308786&req=5

Figure 2: CD11b, CD45 and CD90 expression in rat bone marrow mesenchymal stem cells detected by flow cytometry.(A) Blank control cells. (B-D) FITC labeled CD90, CD11 and CD45 positive cells respectively. FITC: Fluorescein isothiocyanate.
Mentions: Flow cytometry was used to determine the cell surface antigen profile of BMSCs. Analysis showed expression of CD90 in more than 98% of BMSCs, while expression levels of CD11b and CD45 were less than 2% (Figure 2). This indicated the BMSC population has good uniformity and high purity.

Bottom Line: Mitogen-activated protein kinase signaling was identified as one of the key pathways underlying this differentiation process.The present study shows phosphorylated extracellular signal-regulated protein kinase and phosphorylated p38 protein expression was increased after differentiation.Cellular signaling pathway blocking agents, PD98059 and SB203580, inhibited extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways respectively. mRNA and protein expression of the neuronal marker, neuron specific enolase, and neural stem cell marker, nestin, were decreased in bone marrow mesenchymal stem cells after treatment with the active principle region of Buyang Huanwu decoction.

View Article: PubMed Central - PubMed

Affiliation: Ruikang Hospital Attached to Guangxi Traditional Chinese Medicine College, Level-3 Laboratory of Cell Molecular Biology of State Administration of Traditional Chinese Medicine of the People's Republic of China, Nanning 530011, Guangxi Zhuang Autonomous Region, China.

ABSTRACT
Our preliminary studies confirmed that an active principle region of Buyang Huanwu decoction, comprising alkaloid, polysaccharide, aglycon, glucoside and volatile oil, can induce bone marrow mesenchymal stem cell differentiation into neurons. Mitogen-activated protein kinase signaling was identified as one of the key pathways underlying this differentiation process. The present study shows phosphorylated extracellular signal-regulated protein kinase and phosphorylated p38 protein expression was increased after differentiation. Cellular signaling pathway blocking agents, PD98059 and SB203580, inhibited extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways respectively. mRNA and protein expression of the neuronal marker, neuron specific enolase, and neural stem cell marker, nestin, were decreased in bone marrow mesenchymal stem cells after treatment with the active principle region of Buyang Huanwu decoction. Experimental findings indicate that, extracellular signal-regulated protein kinase and p38 in mitogen-activated protein kinase signaling pathways participate in bone marrow mesenchymal stem cell differentiation into neuron-like cells, induced by the active principle region of Buyang Huanwu decoction.

No MeSH data available.


Related in: MedlinePlus