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Alpha B-crystallin improved survival of retinal ganglion cells in a rat model of acute ocular hypertension.

Wu Z, Wang L, Hou S - Neural Regen Res (2012)

Bottom Line: Increased endogenous αB-crystallin protein levels have been shown to reduce cell apoptosis, although the effects of exogenous αB-crystallin protein remain poorly understood.Intravitreal injection of αB-crystallin in the right eye increased the number of retinal ganglion cells and reduced caspase-3 expression.Results demonstrated that exogenous αB-crystallin protein inhibited caspase-3 expression and improved retinal ganglion cell survival following acute ocular hypertension.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, General Hospital of Chinese People's Armed Police Forces, Beijing 100039, China.

ABSTRACT
Increased endogenous αB-crystallin protein levels have been shown to reduce cell apoptosis, although the effects of exogenous αB-crystallin protein remain poorly understood. The present study established an acute ocular hypertension model in the right eye of Sprague-Dawley rats. Fluorogold retrograde tracing and immunofluorescence methods showed that the number of retinal ganglion cells decreased in the right eyes and caspase-3 expression increased following acute ocular hypertension. Intravitreal injection of αB-crystallin in the right eye increased the number of retinal ganglion cells and reduced caspase-3 expression. Results demonstrated that exogenous αB-crystallin protein inhibited caspase-3 expression and improved retinal ganglion cell survival following acute ocular hypertension.

No MeSH data available.


Related in: MedlinePlus

Survival of retinal ganglial cells (RGCs) in rats with acute ocular hypertension (fluorogold labeling, × 200).Acute ocular hypertension decreases the number of RGCs, which gradually decrease with increasing time. Cells with fluorescence spots in the blue background are RGCs. Fluorogold mainly aggregates in the cytoplasm and is detected as a gold staining.
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Figure 1: Survival of retinal ganglial cells (RGCs) in rats with acute ocular hypertension (fluorogold labeling, × 200).Acute ocular hypertension decreases the number of RGCs, which gradually decrease with increasing time. Cells with fluorescence spots in the blue background are RGCs. Fluorogold mainly aggregates in the cytoplasm and is detected as a gold staining.

Mentions: Fluorogold retrograde tracing revealed a decreased number of RGCs in the right eye of model rats, but intravitreal injection of αB-crystallin increased the number of RGCs (P < 0.01). Normal saline injection did not change the number of RGCs in the rat model of acute ocular hypertension (P > 0.05; Table 1, Figure 1).


Alpha B-crystallin improved survival of retinal ganglion cells in a rat model of acute ocular hypertension.

Wu Z, Wang L, Hou S - Neural Regen Res (2012)

Survival of retinal ganglial cells (RGCs) in rats with acute ocular hypertension (fluorogold labeling, × 200).Acute ocular hypertension decreases the number of RGCs, which gradually decrease with increasing time. Cells with fluorescence spots in the blue background are RGCs. Fluorogold mainly aggregates in the cytoplasm and is detected as a gold staining.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308781&req=5

Figure 1: Survival of retinal ganglial cells (RGCs) in rats with acute ocular hypertension (fluorogold labeling, × 200).Acute ocular hypertension decreases the number of RGCs, which gradually decrease with increasing time. Cells with fluorescence spots in the blue background are RGCs. Fluorogold mainly aggregates in the cytoplasm and is detected as a gold staining.
Mentions: Fluorogold retrograde tracing revealed a decreased number of RGCs in the right eye of model rats, but intravitreal injection of αB-crystallin increased the number of RGCs (P < 0.01). Normal saline injection did not change the number of RGCs in the rat model of acute ocular hypertension (P > 0.05; Table 1, Figure 1).

Bottom Line: Increased endogenous αB-crystallin protein levels have been shown to reduce cell apoptosis, although the effects of exogenous αB-crystallin protein remain poorly understood.Intravitreal injection of αB-crystallin in the right eye increased the number of retinal ganglion cells and reduced caspase-3 expression.Results demonstrated that exogenous αB-crystallin protein inhibited caspase-3 expression and improved retinal ganglion cell survival following acute ocular hypertension.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, General Hospital of Chinese People's Armed Police Forces, Beijing 100039, China.

ABSTRACT
Increased endogenous αB-crystallin protein levels have been shown to reduce cell apoptosis, although the effects of exogenous αB-crystallin protein remain poorly understood. The present study established an acute ocular hypertension model in the right eye of Sprague-Dawley rats. Fluorogold retrograde tracing and immunofluorescence methods showed that the number of retinal ganglion cells decreased in the right eyes and caspase-3 expression increased following acute ocular hypertension. Intravitreal injection of αB-crystallin in the right eye increased the number of retinal ganglion cells and reduced caspase-3 expression. Results demonstrated that exogenous αB-crystallin protein inhibited caspase-3 expression and improved retinal ganglion cell survival following acute ocular hypertension.

No MeSH data available.


Related in: MedlinePlus