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Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes.

Zhu G, Sun C, Liu W - Neural Regen Res (2012)

Bottom Line: At 14 days, there were 5.6% nestin-, 9.6% β-III tubulin-, 81.1% glial fibrillary acidic protein-, and 2.2% O4-positive cells.In cells not treated with neurotrophin-3, some were nestin-positive, while the majority showed positive staining for glial fibrillary acidic protein.Our experimental findings indicate that neurotrophin-3 is a crucial factor for inducing neural stem cells differentiation into neurons and oligodendrocytes.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Second Affiliated Hospital of Zhejiang University Medical College, Hangzhou 310009, Zhejiang Province, China.

ABSTRACT
In this study, cells from the cerebral cortex of fetal rats at pregnant 16 days were harvested and cultured with 20 μg/L neurotrophin-3. After 7 days of culture, immunocytochemical staining showed that, 22.4% of cells were positive for nestin, 10.5% were positive for β-III tubulin (neuronal marker), and 60.6% were positive for glial fibrillary acidic protein, but no cells were positive for O4 (oligodendrocytic marker). At 14 days, there were 5.6% nestin-, 9.6% β-III tubulin-, 81.1% glial fibrillary acidic protein-, and 2.2% O4-positive cells. In cells not treated with neurotrophin-3, some were nestin-positive, while the majority showed positive staining for glial fibrillary acidic protein. Our experimental findings indicate that neurotrophin-3 is a crucial factor for inducing neural stem cells differentiation into neurons and oligodendrocytes.

No MeSH data available.


Differentiation of neural stem cells after 14-day induction with neurotrophin-3 (immunocytochemistry, × 400).(A) Anti-nestin; (B) anti-beta III tubulin; (C) anti-glial fibrillary acidic protein; (D) anti-O4. Arrows indicate the positive cells.
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Figure 3: Differentiation of neural stem cells after 14-day induction with neurotrophin-3 (immunocytochemistry, × 400).(A) Anti-nestin; (B) anti-beta III tubulin; (C) anti-glial fibrillary acidic protein; (D) anti-O4. Arrows indicate the positive cells.

Mentions: On day 14, there were 5.6% nestin-positive cells, 9.6% beta-III tubulin-positive cells, 81.1% GFAP-positive cells, and 2.2% O4-positive cells (Figure 3). Control cells (untreated) were most GFAP-positive cells, with a few nestin-positive cells.


Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes.

Zhu G, Sun C, Liu W - Neural Regen Res (2012)

Differentiation of neural stem cells after 14-day induction with neurotrophin-3 (immunocytochemistry, × 400).(A) Anti-nestin; (B) anti-beta III tubulin; (C) anti-glial fibrillary acidic protein; (D) anti-O4. Arrows indicate the positive cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308779&req=5

Figure 3: Differentiation of neural stem cells after 14-day induction with neurotrophin-3 (immunocytochemistry, × 400).(A) Anti-nestin; (B) anti-beta III tubulin; (C) anti-glial fibrillary acidic protein; (D) anti-O4. Arrows indicate the positive cells.
Mentions: On day 14, there were 5.6% nestin-positive cells, 9.6% beta-III tubulin-positive cells, 81.1% GFAP-positive cells, and 2.2% O4-positive cells (Figure 3). Control cells (untreated) were most GFAP-positive cells, with a few nestin-positive cells.

Bottom Line: At 14 days, there were 5.6% nestin-, 9.6% β-III tubulin-, 81.1% glial fibrillary acidic protein-, and 2.2% O4-positive cells.In cells not treated with neurotrophin-3, some were nestin-positive, while the majority showed positive staining for glial fibrillary acidic protein.Our experimental findings indicate that neurotrophin-3 is a crucial factor for inducing neural stem cells differentiation into neurons and oligodendrocytes.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Second Affiliated Hospital of Zhejiang University Medical College, Hangzhou 310009, Zhejiang Province, China.

ABSTRACT
In this study, cells from the cerebral cortex of fetal rats at pregnant 16 days were harvested and cultured with 20 μg/L neurotrophin-3. After 7 days of culture, immunocytochemical staining showed that, 22.4% of cells were positive for nestin, 10.5% were positive for β-III tubulin (neuronal marker), and 60.6% were positive for glial fibrillary acidic protein, but no cells were positive for O4 (oligodendrocytic marker). At 14 days, there were 5.6% nestin-, 9.6% β-III tubulin-, 81.1% glial fibrillary acidic protein-, and 2.2% O4-positive cells. In cells not treated with neurotrophin-3, some were nestin-positive, while the majority showed positive staining for glial fibrillary acidic protein. Our experimental findings indicate that neurotrophin-3 is a crucial factor for inducing neural stem cells differentiation into neurons and oligodendrocytes.

No MeSH data available.