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Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes.

Zhu G, Sun C, Liu W - Neural Regen Res (2012)

Bottom Line: At 14 days, there were 5.6% nestin-, 9.6% β-III tubulin-, 81.1% glial fibrillary acidic protein-, and 2.2% O4-positive cells.In cells not treated with neurotrophin-3, some were nestin-positive, while the majority showed positive staining for glial fibrillary acidic protein.Our experimental findings indicate that neurotrophin-3 is a crucial factor for inducing neural stem cells differentiation into neurons and oligodendrocytes.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Second Affiliated Hospital of Zhejiang University Medical College, Hangzhou 310009, Zhejiang Province, China.

ABSTRACT
In this study, cells from the cerebral cortex of fetal rats at pregnant 16 days were harvested and cultured with 20 μg/L neurotrophin-3. After 7 days of culture, immunocytochemical staining showed that, 22.4% of cells were positive for nestin, 10.5% were positive for β-III tubulin (neuronal marker), and 60.6% were positive for glial fibrillary acidic protein, but no cells were positive for O4 (oligodendrocytic marker). At 14 days, there were 5.6% nestin-, 9.6% β-III tubulin-, 81.1% glial fibrillary acidic protein-, and 2.2% O4-positive cells. In cells not treated with neurotrophin-3, some were nestin-positive, while the majority showed positive staining for glial fibrillary acidic protein. Our experimental findings indicate that neurotrophin-3 is a crucial factor for inducing neural stem cells differentiation into neurons and oligodendrocytes.

No MeSH data available.


Related in: MedlinePlus

Primary culture of neural stem cells with and without neurotrophin-3 (NT-3) induction (phase contrast microscope, × 40).(A) Primary cell culture without NT-3 on day 4, spheres of neural stem cells can be observed.(B) Primary cell culture without NT-3 on day 8, the size and the number of colonies increased. Suspending cells grew into colonies.(C) Primary cell culture with NT-3 on day 8. Cell bodies extended processes and adhered to the dishes after NT-3 induction.
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Figure 1: Primary culture of neural stem cells with and without neurotrophin-3 (NT-3) induction (phase contrast microscope, × 40).(A) Primary cell culture without NT-3 on day 4, spheres of neural stem cells can be observed.(B) Primary cell culture without NT-3 on day 8, the size and the number of colonies increased. Suspending cells grew into colonies.(C) Primary cell culture with NT-3 on day 8. Cell bodies extended processes and adhered to the dishes after NT-3 induction.

Mentions: Primary cultured neural stem cells formed spheres, which were strongly refractive over the first 24 hours, with two or more colony-forming cells present in the culture. On day 4 after primary culture, neural stem cells were placed in an inverted phase contrast microscope, where spheres of neural stem cell clones were observed (Figure 1A). With the prolongation of incubation time, the size and the number of colonies increased. On day 8, the remaining cells in suspension grew into colonies (Figure 1B). The cell bodies extended processes and adhered to the dishes after neurotrophin-3 stimulation (Figure 1C).


Effects of neurotrophin-3 on the differentiation of neural stem cells into neurons and oligodendrocytes.

Zhu G, Sun C, Liu W - Neural Regen Res (2012)

Primary culture of neural stem cells with and without neurotrophin-3 (NT-3) induction (phase contrast microscope, × 40).(A) Primary cell culture without NT-3 on day 4, spheres of neural stem cells can be observed.(B) Primary cell culture without NT-3 on day 8, the size and the number of colonies increased. Suspending cells grew into colonies.(C) Primary cell culture with NT-3 on day 8. Cell bodies extended processes and adhered to the dishes after NT-3 induction.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308779&req=5

Figure 1: Primary culture of neural stem cells with and without neurotrophin-3 (NT-3) induction (phase contrast microscope, × 40).(A) Primary cell culture without NT-3 on day 4, spheres of neural stem cells can be observed.(B) Primary cell culture without NT-3 on day 8, the size and the number of colonies increased. Suspending cells grew into colonies.(C) Primary cell culture with NT-3 on day 8. Cell bodies extended processes and adhered to the dishes after NT-3 induction.
Mentions: Primary cultured neural stem cells formed spheres, which were strongly refractive over the first 24 hours, with two or more colony-forming cells present in the culture. On day 4 after primary culture, neural stem cells were placed in an inverted phase contrast microscope, where spheres of neural stem cell clones were observed (Figure 1A). With the prolongation of incubation time, the size and the number of colonies increased. On day 8, the remaining cells in suspension grew into colonies (Figure 1B). The cell bodies extended processes and adhered to the dishes after neurotrophin-3 stimulation (Figure 1C).

Bottom Line: At 14 days, there were 5.6% nestin-, 9.6% β-III tubulin-, 81.1% glial fibrillary acidic protein-, and 2.2% O4-positive cells.In cells not treated with neurotrophin-3, some were nestin-positive, while the majority showed positive staining for glial fibrillary acidic protein.Our experimental findings indicate that neurotrophin-3 is a crucial factor for inducing neural stem cells differentiation into neurons and oligodendrocytes.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Second Affiliated Hospital of Zhejiang University Medical College, Hangzhou 310009, Zhejiang Province, China.

ABSTRACT
In this study, cells from the cerebral cortex of fetal rats at pregnant 16 days were harvested and cultured with 20 μg/L neurotrophin-3. After 7 days of culture, immunocytochemical staining showed that, 22.4% of cells were positive for nestin, 10.5% were positive for β-III tubulin (neuronal marker), and 60.6% were positive for glial fibrillary acidic protein, but no cells were positive for O4 (oligodendrocytic marker). At 14 days, there were 5.6% nestin-, 9.6% β-III tubulin-, 81.1% glial fibrillary acidic protein-, and 2.2% O4-positive cells. In cells not treated with neurotrophin-3, some were nestin-positive, while the majority showed positive staining for glial fibrillary acidic protein. Our experimental findings indicate that neurotrophin-3 is a crucial factor for inducing neural stem cells differentiation into neurons and oligodendrocytes.

No MeSH data available.


Related in: MedlinePlus