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Wnt3a expression during the differentiation of adipose-derived stem cells into cholinergic neurons.

Liu B, Deng C, Zhang Y, Zhang J - Neural Regen Res (2012)

Bottom Line: Real-time reverse transcription-PCR and western blot assay results demonstrated significantly increased choline acetyltransferase and Wnt3a protein and mRNA expressions, respectively, in adipose-derived stem cells following induction.Choline acetyltransferase expression positively correlated with Wnt3a protein and mRNA expressions.These results demonstrated that neural-induced medium induced differentiation of adipose-derived stem cells into cholinergic neuronal-like cells, with subsequent increased Wnt3a expression.

View Article: PubMed Central - PubMed

Affiliation: First Department of Neurology, Hospital Affiliated to Hebei United University, Tangshan 063000, Hebei Province, China.

ABSTRACT
The present study analyzed changes in Wnt3a expression during differentiation of adipose-derived stem cells into cholinergic neurons. Immunocytochemistry and immunofluorescence revealed significantly increased nestin, neuron-specific enolase, microtubule-associated protein 2, and choline acetyltransferase expression in adipose-derived stem cells isolated from Sprague-Dawley rats and cultured in vitro in neural-induced medium. These expressions increased with prolonged induction time. Real-time reverse transcription-PCR and western blot assay results demonstrated significantly increased choline acetyltransferase and Wnt3a protein and mRNA expressions, respectively, in adipose-derived stem cells following induction. Choline acetyltransferase expression positively correlated with Wnt3a protein and mRNA expressions. These results demonstrated that neural-induced medium induced differentiation of adipose-derived stem cells into cholinergic neuronal-like cells, with subsequent increased Wnt3a expression.

No MeSH data available.


Nestin, neuron-specific enolase (NSE), microtubule-associated protein 2 (MAP2), and choline acetyltransferase (ChAT) expression at 16 hours following induction in neural-induced medium (immunocytochemistry, × 100).(A), (B), and (C), respectively, represent positive nestin, NSE, and MAP2 expression. Yellow particles are visible in the cytoplasm, with blue nuclei. (D) Positive ChAT expression; brown particles are visible in the cytoplasm and nuclei.
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Figure 2: Nestin, neuron-specific enolase (NSE), microtubule-associated protein 2 (MAP2), and choline acetyltransferase (ChAT) expression at 16 hours following induction in neural-induced medium (immunocytochemistry, × 100).(A), (B), and (C), respectively, represent positive nestin, NSE, and MAP2 expression. Yellow particles are visible in the cytoplasm, with blue nuclei. (D) Positive ChAT expression; brown particles are visible in the cytoplasm and nuclei.

Mentions: Immunocytochemistry revealed low expression of nestin, NSE, MAP2, and choline acetyltransferase in non-induced adipose-derived stem cells. Nestin, NSE, MAP2, and choline acetyltransferase expression significantly increased following NIM induction (Figure 2), and expressions continued to increase with prolonged induction time (P < 0.01; Table 1).


Wnt3a expression during the differentiation of adipose-derived stem cells into cholinergic neurons.

Liu B, Deng C, Zhang Y, Zhang J - Neural Regen Res (2012)

Nestin, neuron-specific enolase (NSE), microtubule-associated protein 2 (MAP2), and choline acetyltransferase (ChAT) expression at 16 hours following induction in neural-induced medium (immunocytochemistry, × 100).(A), (B), and (C), respectively, represent positive nestin, NSE, and MAP2 expression. Yellow particles are visible in the cytoplasm, with blue nuclei. (D) Positive ChAT expression; brown particles are visible in the cytoplasm and nuclei.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308776&req=5

Figure 2: Nestin, neuron-specific enolase (NSE), microtubule-associated protein 2 (MAP2), and choline acetyltransferase (ChAT) expression at 16 hours following induction in neural-induced medium (immunocytochemistry, × 100).(A), (B), and (C), respectively, represent positive nestin, NSE, and MAP2 expression. Yellow particles are visible in the cytoplasm, with blue nuclei. (D) Positive ChAT expression; brown particles are visible in the cytoplasm and nuclei.
Mentions: Immunocytochemistry revealed low expression of nestin, NSE, MAP2, and choline acetyltransferase in non-induced adipose-derived stem cells. Nestin, NSE, MAP2, and choline acetyltransferase expression significantly increased following NIM induction (Figure 2), and expressions continued to increase with prolonged induction time (P < 0.01; Table 1).

Bottom Line: Real-time reverse transcription-PCR and western blot assay results demonstrated significantly increased choline acetyltransferase and Wnt3a protein and mRNA expressions, respectively, in adipose-derived stem cells following induction.Choline acetyltransferase expression positively correlated with Wnt3a protein and mRNA expressions.These results demonstrated that neural-induced medium induced differentiation of adipose-derived stem cells into cholinergic neuronal-like cells, with subsequent increased Wnt3a expression.

View Article: PubMed Central - PubMed

Affiliation: First Department of Neurology, Hospital Affiliated to Hebei United University, Tangshan 063000, Hebei Province, China.

ABSTRACT
The present study analyzed changes in Wnt3a expression during differentiation of adipose-derived stem cells into cholinergic neurons. Immunocytochemistry and immunofluorescence revealed significantly increased nestin, neuron-specific enolase, microtubule-associated protein 2, and choline acetyltransferase expression in adipose-derived stem cells isolated from Sprague-Dawley rats and cultured in vitro in neural-induced medium. These expressions increased with prolonged induction time. Real-time reverse transcription-PCR and western blot assay results demonstrated significantly increased choline acetyltransferase and Wnt3a protein and mRNA expressions, respectively, in adipose-derived stem cells following induction. Choline acetyltransferase expression positively correlated with Wnt3a protein and mRNA expressions. These results demonstrated that neural-induced medium induced differentiation of adipose-derived stem cells into cholinergic neuronal-like cells, with subsequent increased Wnt3a expression.

No MeSH data available.