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Wnt3a expression during the differentiation of adipose-derived stem cells into cholinergic neurons.

Liu B, Deng C, Zhang Y, Zhang J - Neural Regen Res (2012)

Bottom Line: Immunocytochemistry and immunofluorescence revealed significantly increased nestin, neuron-specific enolase, microtubule-associated protein 2, and choline acetyltransferase expression in adipose-derived stem cells isolated from Sprague-Dawley rats and cultured in vitro in neural-induced medium.Choline acetyltransferase expression positively correlated with Wnt3a protein and mRNA expressions.These results demonstrated that neural-induced medium induced differentiation of adipose-derived stem cells into cholinergic neuronal-like cells, with subsequent increased Wnt3a expression.

View Article: PubMed Central - PubMed

Affiliation: First Department of Neurology, Hospital Affiliated to Hebei United University, Tangshan 063000, Hebei Province, China.

ABSTRACT
The present study analyzed changes in Wnt3a expression during differentiation of adipose-derived stem cells into cholinergic neurons. Immunocytochemistry and immunofluorescence revealed significantly increased nestin, neuron-specific enolase, microtubule-associated protein 2, and choline acetyltransferase expression in adipose-derived stem cells isolated from Sprague-Dawley rats and cultured in vitro in neural-induced medium. These expressions increased with prolonged induction time. Real-time reverse transcription-PCR and western blot assay results demonstrated significantly increased choline acetyltransferase and Wnt3a protein and mRNA expressions, respectively, in adipose-derived stem cells following induction. Choline acetyltransferase expression positively correlated with Wnt3a protein and mRNA expressions. These results demonstrated that neural-induced medium induced differentiation of adipose-derived stem cells into cholinergic neuronal-like cells, with subsequent increased Wnt3a expression.

No MeSH data available.


Morphology of isolated and cultured rat adipose-derived stem cells (inverted phase-contrast microscope, × 40).(A) Cells grow in a swirled pattern at 7 days after first medium replacement.(B) Cells are suspended with increased refraction.(C) Sub-cultured cells are spindle- and swirl-shaped.(D) Cells following induction in neural-induced medium exhibit neuronal-like morphological changes.
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Figure 1: Morphology of isolated and cultured rat adipose-derived stem cells (inverted phase-contrast microscope, × 40).(A) Cells grow in a swirled pattern at 7 days after first medium replacement.(B) Cells are suspended with increased refraction.(C) Sub-cultured cells are spindle- and swirl-shaped.(D) Cells following induction in neural-induced medium exhibit neuronal-like morphological changes.

Mentions: The present study analyzed changes in Wnt3a expression during adipose-derived stem cell differentiation into cholinergic neurons. In addition, the correlation between Wnt3a and choline acetyltransferase expression was determined, and the effects of Wnt3a on adipose-derived stem cell differentiation into cholinergic neurons were examined.


Wnt3a expression during the differentiation of adipose-derived stem cells into cholinergic neurons.

Liu B, Deng C, Zhang Y, Zhang J - Neural Regen Res (2012)

Morphology of isolated and cultured rat adipose-derived stem cells (inverted phase-contrast microscope, × 40).(A) Cells grow in a swirled pattern at 7 days after first medium replacement.(B) Cells are suspended with increased refraction.(C) Sub-cultured cells are spindle- and swirl-shaped.(D) Cells following induction in neural-induced medium exhibit neuronal-like morphological changes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308776&req=5

Figure 1: Morphology of isolated and cultured rat adipose-derived stem cells (inverted phase-contrast microscope, × 40).(A) Cells grow in a swirled pattern at 7 days after first medium replacement.(B) Cells are suspended with increased refraction.(C) Sub-cultured cells are spindle- and swirl-shaped.(D) Cells following induction in neural-induced medium exhibit neuronal-like morphological changes.
Mentions: The present study analyzed changes in Wnt3a expression during adipose-derived stem cell differentiation into cholinergic neurons. In addition, the correlation between Wnt3a and choline acetyltransferase expression was determined, and the effects of Wnt3a on adipose-derived stem cell differentiation into cholinergic neurons were examined.

Bottom Line: Immunocytochemistry and immunofluorescence revealed significantly increased nestin, neuron-specific enolase, microtubule-associated protein 2, and choline acetyltransferase expression in adipose-derived stem cells isolated from Sprague-Dawley rats and cultured in vitro in neural-induced medium.Choline acetyltransferase expression positively correlated with Wnt3a protein and mRNA expressions.These results demonstrated that neural-induced medium induced differentiation of adipose-derived stem cells into cholinergic neuronal-like cells, with subsequent increased Wnt3a expression.

View Article: PubMed Central - PubMed

Affiliation: First Department of Neurology, Hospital Affiliated to Hebei United University, Tangshan 063000, Hebei Province, China.

ABSTRACT
The present study analyzed changes in Wnt3a expression during differentiation of adipose-derived stem cells into cholinergic neurons. Immunocytochemistry and immunofluorescence revealed significantly increased nestin, neuron-specific enolase, microtubule-associated protein 2, and choline acetyltransferase expression in adipose-derived stem cells isolated from Sprague-Dawley rats and cultured in vitro in neural-induced medium. These expressions increased with prolonged induction time. Real-time reverse transcription-PCR and western blot assay results demonstrated significantly increased choline acetyltransferase and Wnt3a protein and mRNA expressions, respectively, in adipose-derived stem cells following induction. Choline acetyltransferase expression positively correlated with Wnt3a protein and mRNA expressions. These results demonstrated that neural-induced medium induced differentiation of adipose-derived stem cells into cholinergic neuronal-like cells, with subsequent increased Wnt3a expression.

No MeSH data available.