Limits...
Effect of intravenous transplantation of bone marrow mesenchymal stem cells on neurotransmitters and synapsins in rats with spinal cord injury.

Chen S, Wu B, Lin J - Neural Regen Res (2012)

Bottom Line: Bone marrow mesenchymal stem cells were isolated, purified and cultured in vitro by Percoll density gradient centrifugation combined with the cell adherence method.Expressions of choline acetyltransferase, glutamic acid decarboxylase and synapsins in the damaged spinal cord of rats was significantly increased after transplantation, determined by immunofluorescence staining and laser confocal scanning microscopy.The Basso-Beattie- Bresnahan scores positively correlated with expression of choline acetyltransferase and synapsins.

View Article: PubMed Central - PubMed

Affiliation: Department of Human Anatomy and Tissue Embryology, Fujian Medical University, Minhou 350108, Fujian Province, China.

ABSTRACT
Bone marrow mesenchymal stem cells were isolated, purified and cultured in vitro by Percoll density gradient centrifugation combined with the cell adherence method. Passages 3-5 bone marrow mesenchymal stem cells were transplanted into rats with traumatic spinal cord injury via the caudal vein. Basso-Beattie-Bresnahan scores indicate that neurological function of experimental rats was significantly improved over transplantation time (1-5 weeks). Expressions of choline acetyltransferase, glutamic acid decarboxylase and synapsins in the damaged spinal cord of rats was significantly increased after transplantation, determined by immunofluorescence staining and laser confocal scanning microscopy. Bone marrow mesenchymal stem cells that had migrated into the damaged area of rats in the experimental group began to express choline acetyltransferase, glutamic acid decarboxylase and synapsins, 3 weeks after transplantation. The Basso-Beattie- Bresnahan scores positively correlated with expression of choline acetyltransferase and synapsins. Experimental findings indicate that intravenously transplanted bone marrow mesenchymal stem cells traverse into the damaged spinal cord of rats, promote expression of choline acetyltransferase, glutamic acid decarboxylase and synapsins, and improve nerve function in rats with spinal cord injury.

No MeSH data available.


Related in: MedlinePlus

Synapsins expression in bone marrow mesenchymal stem cells (BMSCs) migrating to the injured spinal cord (immunofluorescence double staining, laser confocal microscope, × 100).At 1 week after transplantation, transplanted BMSCs [carboxyfluorescein succinimidyl ester (CFSE) marker; green] did not express synapsins [tetramethylrhodamine isothiocyanate (TRITC) staining; red]; at 3 weeks after transplantation, BMSCs began to express synapsins (yellow).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4308773&req=5

Figure 8: Synapsins expression in bone marrow mesenchymal stem cells (BMSCs) migrating to the injured spinal cord (immunofluorescence double staining, laser confocal microscope, × 100).At 1 week after transplantation, transplanted BMSCs [carboxyfluorescein succinimidyl ester (CFSE) marker; green] did not express synapsins [tetramethylrhodamine isothiocyanate (TRITC) staining; red]; at 3 weeks after transplantation, BMSCs began to express synapsins (yellow).

Mentions: Using laser scanning confocal microscopy, transplanted bone marrow mesenchymal stem cells (green fluorescence; carboxyfluorescein succinimidyl ester-labeled) and cells expressing choline acetyltransferase, glutamic acid decarboxylase and synapsins (red fluorescence; tetramethylrhodamine isothiocyanate-labeled) were observed. When the two signals overlapped, cells exhibited yellow fluorescence. At week 1 after transplantation, transplanted bone marrow mesenchymal stem cells with green fluorescence and nerve cells with red fluorescence were visible in the spinal cord of rats in the experimental group, but were not observed to overlap. At weeks 3 and 5 after transplantation, yellow fluorescence was visible in the gray matter of the spinal cord in the experimental rats, indicating that the transplanted bone marrow mesenchymal stem cells began to express choline acetyltransferase, glutamic acid decarboxylase and synapsins (Figures 6–8).


Effect of intravenous transplantation of bone marrow mesenchymal stem cells on neurotransmitters and synapsins in rats with spinal cord injury.

Chen S, Wu B, Lin J - Neural Regen Res (2012)

Synapsins expression in bone marrow mesenchymal stem cells (BMSCs) migrating to the injured spinal cord (immunofluorescence double staining, laser confocal microscope, × 100).At 1 week after transplantation, transplanted BMSCs [carboxyfluorescein succinimidyl ester (CFSE) marker; green] did not express synapsins [tetramethylrhodamine isothiocyanate (TRITC) staining; red]; at 3 weeks after transplantation, BMSCs began to express synapsins (yellow).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308773&req=5

Figure 8: Synapsins expression in bone marrow mesenchymal stem cells (BMSCs) migrating to the injured spinal cord (immunofluorescence double staining, laser confocal microscope, × 100).At 1 week after transplantation, transplanted BMSCs [carboxyfluorescein succinimidyl ester (CFSE) marker; green] did not express synapsins [tetramethylrhodamine isothiocyanate (TRITC) staining; red]; at 3 weeks after transplantation, BMSCs began to express synapsins (yellow).
Mentions: Using laser scanning confocal microscopy, transplanted bone marrow mesenchymal stem cells (green fluorescence; carboxyfluorescein succinimidyl ester-labeled) and cells expressing choline acetyltransferase, glutamic acid decarboxylase and synapsins (red fluorescence; tetramethylrhodamine isothiocyanate-labeled) were observed. When the two signals overlapped, cells exhibited yellow fluorescence. At week 1 after transplantation, transplanted bone marrow mesenchymal stem cells with green fluorescence and nerve cells with red fluorescence were visible in the spinal cord of rats in the experimental group, but were not observed to overlap. At weeks 3 and 5 after transplantation, yellow fluorescence was visible in the gray matter of the spinal cord in the experimental rats, indicating that the transplanted bone marrow mesenchymal stem cells began to express choline acetyltransferase, glutamic acid decarboxylase and synapsins (Figures 6–8).

Bottom Line: Bone marrow mesenchymal stem cells were isolated, purified and cultured in vitro by Percoll density gradient centrifugation combined with the cell adherence method.Expressions of choline acetyltransferase, glutamic acid decarboxylase and synapsins in the damaged spinal cord of rats was significantly increased after transplantation, determined by immunofluorescence staining and laser confocal scanning microscopy.The Basso-Beattie- Bresnahan scores positively correlated with expression of choline acetyltransferase and synapsins.

View Article: PubMed Central - PubMed

Affiliation: Department of Human Anatomy and Tissue Embryology, Fujian Medical University, Minhou 350108, Fujian Province, China.

ABSTRACT
Bone marrow mesenchymal stem cells were isolated, purified and cultured in vitro by Percoll density gradient centrifugation combined with the cell adherence method. Passages 3-5 bone marrow mesenchymal stem cells were transplanted into rats with traumatic spinal cord injury via the caudal vein. Basso-Beattie-Bresnahan scores indicate that neurological function of experimental rats was significantly improved over transplantation time (1-5 weeks). Expressions of choline acetyltransferase, glutamic acid decarboxylase and synapsins in the damaged spinal cord of rats was significantly increased after transplantation, determined by immunofluorescence staining and laser confocal scanning microscopy. Bone marrow mesenchymal stem cells that had migrated into the damaged area of rats in the experimental group began to express choline acetyltransferase, glutamic acid decarboxylase and synapsins, 3 weeks after transplantation. The Basso-Beattie- Bresnahan scores positively correlated with expression of choline acetyltransferase and synapsins. Experimental findings indicate that intravenously transplanted bone marrow mesenchymal stem cells traverse into the damaged spinal cord of rats, promote expression of choline acetyltransferase, glutamic acid decarboxylase and synapsins, and improve nerve function in rats with spinal cord injury.

No MeSH data available.


Related in: MedlinePlus