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Effect of intravenous transplantation of bone marrow mesenchymal stem cells on neurotransmitters and synapsins in rats with spinal cord injury.

Chen S, Wu B, Lin J - Neural Regen Res (2012)

Bottom Line: Bone marrow mesenchymal stem cells were isolated, purified and cultured in vitro by Percoll density gradient centrifugation combined with the cell adherence method.Expressions of choline acetyltransferase, glutamic acid decarboxylase and synapsins in the damaged spinal cord of rats was significantly increased after transplantation, determined by immunofluorescence staining and laser confocal scanning microscopy.The Basso-Beattie- Bresnahan scores positively correlated with expression of choline acetyltransferase and synapsins.

View Article: PubMed Central - PubMed

Affiliation: Department of Human Anatomy and Tissue Embryology, Fujian Medical University, Minhou 350108, Fujian Province, China.

ABSTRACT
Bone marrow mesenchymal stem cells were isolated, purified and cultured in vitro by Percoll density gradient centrifugation combined with the cell adherence method. Passages 3-5 bone marrow mesenchymal stem cells were transplanted into rats with traumatic spinal cord injury via the caudal vein. Basso-Beattie-Bresnahan scores indicate that neurological function of experimental rats was significantly improved over transplantation time (1-5 weeks). Expressions of choline acetyltransferase, glutamic acid decarboxylase and synapsins in the damaged spinal cord of rats was significantly increased after transplantation, determined by immunofluorescence staining and laser confocal scanning microscopy. Bone marrow mesenchymal stem cells that had migrated into the damaged area of rats in the experimental group began to express choline acetyltransferase, glutamic acid decarboxylase and synapsins, 3 weeks after transplantation. The Basso-Beattie- Bresnahan scores positively correlated with expression of choline acetyltransferase and synapsins. Experimental findings indicate that intravenously transplanted bone marrow mesenchymal stem cells traverse into the damaged spinal cord of rats, promote expression of choline acetyltransferase, glutamic acid decarboxylase and synapsins, and improve nerve function in rats with spinal cord injury.

No MeSH data available.


Related in: MedlinePlus

Glutamic acid decarboxylase expression in bone marrow mesenchymal stem cells (BMSCs) migrating to the injured spinal cord (immunofluorescence double staining, laser confocal microscope, × 100).At 1 week after transplantation, transplanted BMSCs [carboxyfluorescein succinimidyl ester (CFSE) marker; green] did not express glutamic acid decarboxylase [tetramethylrhodamine isothiocyanate (TRITC) staining; red]; at 3 weeks after transplantation, BMSCs began to express glutamic acid decarboxylase (yellow).
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Figure 7: Glutamic acid decarboxylase expression in bone marrow mesenchymal stem cells (BMSCs) migrating to the injured spinal cord (immunofluorescence double staining, laser confocal microscope, × 100).At 1 week after transplantation, transplanted BMSCs [carboxyfluorescein succinimidyl ester (CFSE) marker; green] did not express glutamic acid decarboxylase [tetramethylrhodamine isothiocyanate (TRITC) staining; red]; at 3 weeks after transplantation, BMSCs began to express glutamic acid decarboxylase (yellow).


Effect of intravenous transplantation of bone marrow mesenchymal stem cells on neurotransmitters and synapsins in rats with spinal cord injury.

Chen S, Wu B, Lin J - Neural Regen Res (2012)

Glutamic acid decarboxylase expression in bone marrow mesenchymal stem cells (BMSCs) migrating to the injured spinal cord (immunofluorescence double staining, laser confocal microscope, × 100).At 1 week after transplantation, transplanted BMSCs [carboxyfluorescein succinimidyl ester (CFSE) marker; green] did not express glutamic acid decarboxylase [tetramethylrhodamine isothiocyanate (TRITC) staining; red]; at 3 weeks after transplantation, BMSCs began to express glutamic acid decarboxylase (yellow).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308773&req=5

Figure 7: Glutamic acid decarboxylase expression in bone marrow mesenchymal stem cells (BMSCs) migrating to the injured spinal cord (immunofluorescence double staining, laser confocal microscope, × 100).At 1 week after transplantation, transplanted BMSCs [carboxyfluorescein succinimidyl ester (CFSE) marker; green] did not express glutamic acid decarboxylase [tetramethylrhodamine isothiocyanate (TRITC) staining; red]; at 3 weeks after transplantation, BMSCs began to express glutamic acid decarboxylase (yellow).
Bottom Line: Bone marrow mesenchymal stem cells were isolated, purified and cultured in vitro by Percoll density gradient centrifugation combined with the cell adherence method.Expressions of choline acetyltransferase, glutamic acid decarboxylase and synapsins in the damaged spinal cord of rats was significantly increased after transplantation, determined by immunofluorescence staining and laser confocal scanning microscopy.The Basso-Beattie- Bresnahan scores positively correlated with expression of choline acetyltransferase and synapsins.

View Article: PubMed Central - PubMed

Affiliation: Department of Human Anatomy and Tissue Embryology, Fujian Medical University, Minhou 350108, Fujian Province, China.

ABSTRACT
Bone marrow mesenchymal stem cells were isolated, purified and cultured in vitro by Percoll density gradient centrifugation combined with the cell adherence method. Passages 3-5 bone marrow mesenchymal stem cells were transplanted into rats with traumatic spinal cord injury via the caudal vein. Basso-Beattie-Bresnahan scores indicate that neurological function of experimental rats was significantly improved over transplantation time (1-5 weeks). Expressions of choline acetyltransferase, glutamic acid decarboxylase and synapsins in the damaged spinal cord of rats was significantly increased after transplantation, determined by immunofluorescence staining and laser confocal scanning microscopy. Bone marrow mesenchymal stem cells that had migrated into the damaged area of rats in the experimental group began to express choline acetyltransferase, glutamic acid decarboxylase and synapsins, 3 weeks after transplantation. The Basso-Beattie- Bresnahan scores positively correlated with expression of choline acetyltransferase and synapsins. Experimental findings indicate that intravenously transplanted bone marrow mesenchymal stem cells traverse into the damaged spinal cord of rats, promote expression of choline acetyltransferase, glutamic acid decarboxylase and synapsins, and improve nerve function in rats with spinal cord injury.

No MeSH data available.


Related in: MedlinePlus