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Effect of intravenous transplantation of bone marrow mesenchymal stem cells on neurotransmitters and synapsins in rats with spinal cord injury.

Chen S, Wu B, Lin J - Neural Regen Res (2012)

Bottom Line: Bone marrow mesenchymal stem cells were isolated, purified and cultured in vitro by Percoll density gradient centrifugation combined with the cell adherence method.Expressions of choline acetyltransferase, glutamic acid decarboxylase and synapsins in the damaged spinal cord of rats was significantly increased after transplantation, determined by immunofluorescence staining and laser confocal scanning microscopy.The Basso-Beattie- Bresnahan scores positively correlated with expression of choline acetyltransferase and synapsins.

View Article: PubMed Central - PubMed

Affiliation: Department of Human Anatomy and Tissue Embryology, Fujian Medical University, Minhou 350108, Fujian Province, China.

ABSTRACT
Bone marrow mesenchymal stem cells were isolated, purified and cultured in vitro by Percoll density gradient centrifugation combined with the cell adherence method. Passages 3-5 bone marrow mesenchymal stem cells were transplanted into rats with traumatic spinal cord injury via the caudal vein. Basso-Beattie-Bresnahan scores indicate that neurological function of experimental rats was significantly improved over transplantation time (1-5 weeks). Expressions of choline acetyltransferase, glutamic acid decarboxylase and synapsins in the damaged spinal cord of rats was significantly increased after transplantation, determined by immunofluorescence staining and laser confocal scanning microscopy. Bone marrow mesenchymal stem cells that had migrated into the damaged area of rats in the experimental group began to express choline acetyltransferase, glutamic acid decarboxylase and synapsins, 3 weeks after transplantation. The Basso-Beattie- Bresnahan scores positively correlated with expression of choline acetyltransferase and synapsins. Experimental findings indicate that intravenously transplanted bone marrow mesenchymal stem cells traverse into the damaged spinal cord of rats, promote expression of choline acetyltransferase, glutamic acid decarboxylase and synapsins, and improve nerve function in rats with spinal cord injury.

No MeSH data available.


Related in: MedlinePlus

Expression of neurotransmitters and synapsins in the spinal gray matter of rats 3 weeks after transplantation (inverted fluorescence microscope, immunofluorescence staining, × 100).Choline acetyltransferase (A), glutamic acid decarboxylase (B) and synapsins (C) were expressed in the spinal gray matter. Positive products are tetramethylrhodamine isothiocyanate-labeled for red fluorescence.
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Figure 5: Expression of neurotransmitters and synapsins in the spinal gray matter of rats 3 weeks after transplantation (inverted fluorescence microscope, immunofluorescence staining, × 100).Choline acetyltransferase (A), glutamic acid decarboxylase (B) and synapsins (C) were expressed in the spinal gray matter. Positive products are tetramethylrhodamine isothiocyanate-labeled for red fluorescence.

Mentions: Using inverted fluorescence microscopy, cells expressing choline acetyltransferase, glutamic acid decarboxylase and synapsins showed red fluorescence in the cytoplasm and were observed in the gray matter of rats from different groups (Figure 5).


Effect of intravenous transplantation of bone marrow mesenchymal stem cells on neurotransmitters and synapsins in rats with spinal cord injury.

Chen S, Wu B, Lin J - Neural Regen Res (2012)

Expression of neurotransmitters and synapsins in the spinal gray matter of rats 3 weeks after transplantation (inverted fluorescence microscope, immunofluorescence staining, × 100).Choline acetyltransferase (A), glutamic acid decarboxylase (B) and synapsins (C) were expressed in the spinal gray matter. Positive products are tetramethylrhodamine isothiocyanate-labeled for red fluorescence.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308773&req=5

Figure 5: Expression of neurotransmitters and synapsins in the spinal gray matter of rats 3 weeks after transplantation (inverted fluorescence microscope, immunofluorescence staining, × 100).Choline acetyltransferase (A), glutamic acid decarboxylase (B) and synapsins (C) were expressed in the spinal gray matter. Positive products are tetramethylrhodamine isothiocyanate-labeled for red fluorescence.
Mentions: Using inverted fluorescence microscopy, cells expressing choline acetyltransferase, glutamic acid decarboxylase and synapsins showed red fluorescence in the cytoplasm and were observed in the gray matter of rats from different groups (Figure 5).

Bottom Line: Bone marrow mesenchymal stem cells were isolated, purified and cultured in vitro by Percoll density gradient centrifugation combined with the cell adherence method.Expressions of choline acetyltransferase, glutamic acid decarboxylase and synapsins in the damaged spinal cord of rats was significantly increased after transplantation, determined by immunofluorescence staining and laser confocal scanning microscopy.The Basso-Beattie- Bresnahan scores positively correlated with expression of choline acetyltransferase and synapsins.

View Article: PubMed Central - PubMed

Affiliation: Department of Human Anatomy and Tissue Embryology, Fujian Medical University, Minhou 350108, Fujian Province, China.

ABSTRACT
Bone marrow mesenchymal stem cells were isolated, purified and cultured in vitro by Percoll density gradient centrifugation combined with the cell adherence method. Passages 3-5 bone marrow mesenchymal stem cells were transplanted into rats with traumatic spinal cord injury via the caudal vein. Basso-Beattie-Bresnahan scores indicate that neurological function of experimental rats was significantly improved over transplantation time (1-5 weeks). Expressions of choline acetyltransferase, glutamic acid decarboxylase and synapsins in the damaged spinal cord of rats was significantly increased after transplantation, determined by immunofluorescence staining and laser confocal scanning microscopy. Bone marrow mesenchymal stem cells that had migrated into the damaged area of rats in the experimental group began to express choline acetyltransferase, glutamic acid decarboxylase and synapsins, 3 weeks after transplantation. The Basso-Beattie- Bresnahan scores positively correlated with expression of choline acetyltransferase and synapsins. Experimental findings indicate that intravenously transplanted bone marrow mesenchymal stem cells traverse into the damaged spinal cord of rats, promote expression of choline acetyltransferase, glutamic acid decarboxylase and synapsins, and improve nerve function in rats with spinal cord injury.

No MeSH data available.


Related in: MedlinePlus