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Effect of intravenous transplantation of bone marrow mesenchymal stem cells on neurotransmitters and synapsins in rats with spinal cord injury.

Chen S, Wu B, Lin J - Neural Regen Res (2012)

Bottom Line: Bone marrow mesenchymal stem cells were isolated, purified and cultured in vitro by Percoll density gradient centrifugation combined with the cell adherence method.Expressions of choline acetyltransferase, glutamic acid decarboxylase and synapsins in the damaged spinal cord of rats was significantly increased after transplantation, determined by immunofluorescence staining and laser confocal scanning microscopy.The Basso-Beattie- Bresnahan scores positively correlated with expression of choline acetyltransferase and synapsins.

View Article: PubMed Central - PubMed

Affiliation: Department of Human Anatomy and Tissue Embryology, Fujian Medical University, Minhou 350108, Fujian Province, China.

ABSTRACT
Bone marrow mesenchymal stem cells were isolated, purified and cultured in vitro by Percoll density gradient centrifugation combined with the cell adherence method. Passages 3-5 bone marrow mesenchymal stem cells were transplanted into rats with traumatic spinal cord injury via the caudal vein. Basso-Beattie-Bresnahan scores indicate that neurological function of experimental rats was significantly improved over transplantation time (1-5 weeks). Expressions of choline acetyltransferase, glutamic acid decarboxylase and synapsins in the damaged spinal cord of rats was significantly increased after transplantation, determined by immunofluorescence staining and laser confocal scanning microscopy. Bone marrow mesenchymal stem cells that had migrated into the damaged area of rats in the experimental group began to express choline acetyltransferase, glutamic acid decarboxylase and synapsins, 3 weeks after transplantation. The Basso-Beattie- Bresnahan scores positively correlated with expression of choline acetyltransferase and synapsins. Experimental findings indicate that intravenously transplanted bone marrow mesenchymal stem cells traverse into the damaged spinal cord of rats, promote expression of choline acetyltransferase, glutamic acid decarboxylase and synapsins, and improve nerve function in rats with spinal cord injury.

No MeSH data available.


Related in: MedlinePlus

Morphology of bone marrow mesenchymal stem cells cultured in vitro (inverted phase contrast microscope, × 100).(A) Primary cells; (B) passage 3 cells.
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Figure 1: Morphology of bone marrow mesenchymal stem cells cultured in vitro (inverted phase contrast microscope, × 100).(A) Primary cells; (B) passage 3 cells.

Mentions: Bone marrow mesenchymal stem cells began to adhere to the vessel wall 24 hours after culture. They displayed a macromonocyte and fusiform morphology, with polygonal or star-shaped processes. Cells rapidly proliferated as single or multiple cell clones. By days 12–14 after culture, cells reached more than 90% confluence and were predominantly spindle-shaped. There were 1–2 × 106 bone marrow mesenchymal stem cells after primary culture and 1 × 1011 bone marrow mesenchymal stem cells after five passages (Figure 1). Ten cell samples at passage 2 were characterized by flow cytometry and were found to negatively express hematopoietic markers, CD34 and CD45 and positively express mesenchymal markers, CD29 and CD90 (Figure 2).


Effect of intravenous transplantation of bone marrow mesenchymal stem cells on neurotransmitters and synapsins in rats with spinal cord injury.

Chen S, Wu B, Lin J - Neural Regen Res (2012)

Morphology of bone marrow mesenchymal stem cells cultured in vitro (inverted phase contrast microscope, × 100).(A) Primary cells; (B) passage 3 cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308773&req=5

Figure 1: Morphology of bone marrow mesenchymal stem cells cultured in vitro (inverted phase contrast microscope, × 100).(A) Primary cells; (B) passage 3 cells.
Mentions: Bone marrow mesenchymal stem cells began to adhere to the vessel wall 24 hours after culture. They displayed a macromonocyte and fusiform morphology, with polygonal or star-shaped processes. Cells rapidly proliferated as single or multiple cell clones. By days 12–14 after culture, cells reached more than 90% confluence and were predominantly spindle-shaped. There were 1–2 × 106 bone marrow mesenchymal stem cells after primary culture and 1 × 1011 bone marrow mesenchymal stem cells after five passages (Figure 1). Ten cell samples at passage 2 were characterized by flow cytometry and were found to negatively express hematopoietic markers, CD34 and CD45 and positively express mesenchymal markers, CD29 and CD90 (Figure 2).

Bottom Line: Bone marrow mesenchymal stem cells were isolated, purified and cultured in vitro by Percoll density gradient centrifugation combined with the cell adherence method.Expressions of choline acetyltransferase, glutamic acid decarboxylase and synapsins in the damaged spinal cord of rats was significantly increased after transplantation, determined by immunofluorescence staining and laser confocal scanning microscopy.The Basso-Beattie- Bresnahan scores positively correlated with expression of choline acetyltransferase and synapsins.

View Article: PubMed Central - PubMed

Affiliation: Department of Human Anatomy and Tissue Embryology, Fujian Medical University, Minhou 350108, Fujian Province, China.

ABSTRACT
Bone marrow mesenchymal stem cells were isolated, purified and cultured in vitro by Percoll density gradient centrifugation combined with the cell adherence method. Passages 3-5 bone marrow mesenchymal stem cells were transplanted into rats with traumatic spinal cord injury via the caudal vein. Basso-Beattie-Bresnahan scores indicate that neurological function of experimental rats was significantly improved over transplantation time (1-5 weeks). Expressions of choline acetyltransferase, glutamic acid decarboxylase and synapsins in the damaged spinal cord of rats was significantly increased after transplantation, determined by immunofluorescence staining and laser confocal scanning microscopy. Bone marrow mesenchymal stem cells that had migrated into the damaged area of rats in the experimental group began to express choline acetyltransferase, glutamic acid decarboxylase and synapsins, 3 weeks after transplantation. The Basso-Beattie- Bresnahan scores positively correlated with expression of choline acetyltransferase and synapsins. Experimental findings indicate that intravenously transplanted bone marrow mesenchymal stem cells traverse into the damaged spinal cord of rats, promote expression of choline acetyltransferase, glutamic acid decarboxylase and synapsins, and improve nerve function in rats with spinal cord injury.

No MeSH data available.


Related in: MedlinePlus