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Heshouwu decoction, a Chinese herb for tonifying kidney, ameliorates hypothalamic-pituitary- testicular axis secretion in aging rats.

Niu S, Kou S, Zhou X, Ding L - Neural Regen Res (2012)

Bottom Line: An increasing amount of evidence demonstrates the anti-aging effect of Heshouwu in pill form.Heshouwu decoction pretreatment and high-dose Heshouwu decoction had the greatest anti-aging effects.These experimental findings indicate that Heshouwu decoction can improve hypothalamic-pituitary-testicular axis secretion in a subacute aging rat model, and prevent and delay gonadal axis aging, with an effect superior to that of Heshouwu pill.

View Article: PubMed Central - PubMed

Affiliation: School of Basic Medical Science, Hebei University, Baoding 071000, Hebei Province, China.

ABSTRACT
An increasing amount of evidence demonstrates the anti-aging effect of Heshouwu in pill form. In this study, a subacute aging rat model was established by continuous intraperitoneal injection of D-galactose and treated with Heshouwu decoction (a Chinese herb for tonifying the kidney, comprising Heshouwu pill, Herba Epimedii, Radix Salviae Miltiorrhiae, and Poria). Heshouwu pill treated rats were the positive control group. Radioimmunoassay, immunohistochemical staining, and western blot assay showed hypothalamic gonadotropin-releasing hormone, hypothalamic substance P, and serum gonadotropin levels to be significantly increased in the model rats; the concentrations of hypothalamic β-endorphin, and serum levels of insulin-like growth factor 1 and testosterone were significantly decreased. 17β- and 3β-hydroxysteroid dehydrogenase expression in testicular tissue was also decreased. Intragastric administration of Heshouwu decoction at high (9.6 g/mL/100 g), medium (4.8 g/mL/100 g), and low (2.4 g/mL/100 g) doses, Heshouwu decoction pretreatment at a medium dose (4.8 g/mL/100 g), and Heshouwu pill (2.06 g/mL/100 g) significantly reversed these changes. Heshouwu decoction pretreatment and high-dose Heshouwu decoction had the greatest anti-aging effects. These experimental findings indicate that Heshouwu decoction can improve hypothalamic-pituitary-testicular axis secretion in a subacute aging rat model, and prevent and delay gonadal axis aging, with an effect superior to that of Heshouwu pill.

No MeSH data available.


Related in: MedlinePlus

17β-HSD and 3β-HSD protein expression in testicular tissue.1: Model group; 2: normal group; 3: Heshouwu decoction pretreatment group; 4: high-dose Heshouwu decoction group; 5: medium-dose Heshouwu decoction group; 6: low-dose Heshouwu decoction group; 7: Heshouwu pill group; 8: spontaneous recovery group.17β-HSD and 3β-HSD protein expression was greatest in the Heshouwu decoction pretreatment and high-dose Heshouwu decoction groups.HSD: Hydroxysteroid dehydrogenase.
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Figure 2: 17β-HSD and 3β-HSD protein expression in testicular tissue.1: Model group; 2: normal group; 3: Heshouwu decoction pretreatment group; 4: high-dose Heshouwu decoction group; 5: medium-dose Heshouwu decoction group; 6: low-dose Heshouwu decoction group; 7: Heshouwu pill group; 8: spontaneous recovery group.17β-HSD and 3β-HSD protein expression was greatest in the Heshouwu decoction pretreatment and high-dose Heshouwu decoction groups.HSD: Hydroxysteroid dehydrogenase.

Mentions: Immunohistochemical staining showed that 17β-HSD and 3β-HSD were expressed in a dispersed pattern in the cytoplasm of rat Leydig cells. Semi-quantitative analysis by western blot assay demonstrated that 17β-HSD and 3β-HSD protein expression in the model rats was significantly lower than that in the normal group (P < 0.01). Expression was increased to varying degrees in the Heshouwu decoction pretreatment group, the Heshouwu decoction treatment groups, and the Heshouwu pill group (P < 0.05). Levels in the Heshouwu decoction pretreatment group and the high-dose Heshouwu decoction treatment group were higher than those in the other groups (P < 0.05; Table 1, Figures 1 and 2).


Heshouwu decoction, a Chinese herb for tonifying kidney, ameliorates hypothalamic-pituitary- testicular axis secretion in aging rats.

Niu S, Kou S, Zhou X, Ding L - Neural Regen Res (2012)

17β-HSD and 3β-HSD protein expression in testicular tissue.1: Model group; 2: normal group; 3: Heshouwu decoction pretreatment group; 4: high-dose Heshouwu decoction group; 5: medium-dose Heshouwu decoction group; 6: low-dose Heshouwu decoction group; 7: Heshouwu pill group; 8: spontaneous recovery group.17β-HSD and 3β-HSD protein expression was greatest in the Heshouwu decoction pretreatment and high-dose Heshouwu decoction groups.HSD: Hydroxysteroid dehydrogenase.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308762&req=5

Figure 2: 17β-HSD and 3β-HSD protein expression in testicular tissue.1: Model group; 2: normal group; 3: Heshouwu decoction pretreatment group; 4: high-dose Heshouwu decoction group; 5: medium-dose Heshouwu decoction group; 6: low-dose Heshouwu decoction group; 7: Heshouwu pill group; 8: spontaneous recovery group.17β-HSD and 3β-HSD protein expression was greatest in the Heshouwu decoction pretreatment and high-dose Heshouwu decoction groups.HSD: Hydroxysteroid dehydrogenase.
Mentions: Immunohistochemical staining showed that 17β-HSD and 3β-HSD were expressed in a dispersed pattern in the cytoplasm of rat Leydig cells. Semi-quantitative analysis by western blot assay demonstrated that 17β-HSD and 3β-HSD protein expression in the model rats was significantly lower than that in the normal group (P < 0.01). Expression was increased to varying degrees in the Heshouwu decoction pretreatment group, the Heshouwu decoction treatment groups, and the Heshouwu pill group (P < 0.05). Levels in the Heshouwu decoction pretreatment group and the high-dose Heshouwu decoction treatment group were higher than those in the other groups (P < 0.05; Table 1, Figures 1 and 2).

Bottom Line: An increasing amount of evidence demonstrates the anti-aging effect of Heshouwu in pill form.Heshouwu decoction pretreatment and high-dose Heshouwu decoction had the greatest anti-aging effects.These experimental findings indicate that Heshouwu decoction can improve hypothalamic-pituitary-testicular axis secretion in a subacute aging rat model, and prevent and delay gonadal axis aging, with an effect superior to that of Heshouwu pill.

View Article: PubMed Central - PubMed

Affiliation: School of Basic Medical Science, Hebei University, Baoding 071000, Hebei Province, China.

ABSTRACT
An increasing amount of evidence demonstrates the anti-aging effect of Heshouwu in pill form. In this study, a subacute aging rat model was established by continuous intraperitoneal injection of D-galactose and treated with Heshouwu decoction (a Chinese herb for tonifying the kidney, comprising Heshouwu pill, Herba Epimedii, Radix Salviae Miltiorrhiae, and Poria). Heshouwu pill treated rats were the positive control group. Radioimmunoassay, immunohistochemical staining, and western blot assay showed hypothalamic gonadotropin-releasing hormone, hypothalamic substance P, and serum gonadotropin levels to be significantly increased in the model rats; the concentrations of hypothalamic β-endorphin, and serum levels of insulin-like growth factor 1 and testosterone were significantly decreased. 17β- and 3β-hydroxysteroid dehydrogenase expression in testicular tissue was also decreased. Intragastric administration of Heshouwu decoction at high (9.6 g/mL/100 g), medium (4.8 g/mL/100 g), and low (2.4 g/mL/100 g) doses, Heshouwu decoction pretreatment at a medium dose (4.8 g/mL/100 g), and Heshouwu pill (2.06 g/mL/100 g) significantly reversed these changes. Heshouwu decoction pretreatment and high-dose Heshouwu decoction had the greatest anti-aging effects. These experimental findings indicate that Heshouwu decoction can improve hypothalamic-pituitary-testicular axis secretion in a subacute aging rat model, and prevent and delay gonadal axis aging, with an effect superior to that of Heshouwu pill.

No MeSH data available.


Related in: MedlinePlus