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Buyang Huanwu decoction enhances cell membrane fluidity in rats with cerebral ischemia/reperfusion.

Li C - Neural Regen Res (2012)

Bottom Line: After bilateral carotid artery occlusion for 30 minutes and reperfusion for 2 hours, distinct pathological changes presented in the cerebral cortex and cerebellum of rats.Compared with normal rats, nerve cell membrane fluidity significantly decreased in ischemia/reperfusion rats as detected by spin-labeling electron spin resonance, consistent with order parameter S and rotational correlation time τc measurements.Results showed that Buyang Huanwu decoction gradually increased membrane fluidity dose-dependently to normal levels, and eliminated hydroxide (OH(·)) and superoxide ( O2 (·)) free radicals dose-dependently.

View Article: PubMed Central - PubMed

Affiliation: Institute of Brain Sciences, Department of Physiology, Medical College, Datong University, Datong 037009, Shanxi Province, China.

ABSTRACT
After bilateral carotid artery occlusion for 30 minutes and reperfusion for 2 hours, distinct pathological changes presented in the cerebral cortex and cerebellum of rats. Compared with normal rats, nerve cell membrane fluidity significantly decreased in ischemia/reperfusion rats as detected by spin-labeling electron spin resonance, consistent with order parameter S and rotational correlation time τc measurements. Brain nerve cells from rats with ischemia/reperfusion injury were cultured with 1-100 mg/mL Buyang Huanwu decoction. Results showed that Buyang Huanwu decoction gradually increased membrane fluidity dose-dependently to normal levels, and eliminated hydroxide (OH(·)) and superoxide ( O2 (·)) free radicals dose-dependently. These findings suggest that Buyang Huanwu decoction can protect against cell membrane fluidity changes in rats with ischemia/ reperfusion injury by scavenging free radicals.

No MeSH data available.


Related in: MedlinePlus

Correlation between Buyang Huanwu decoction (BYHWD) and the order parameter S in the model group (r = 0.82).Statistical analysis was performed by Pearson correlation analysis.
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Figure 2: Correlation between Buyang Huanwu decoction (BYHWD) and the order parameter S in the model group (r = 0.82).Statistical analysis was performed by Pearson correlation analysis.

Mentions: This indicated that cell membrane fluidity declined and cell function was affected in stroke model rats. After increasing doses of Buyang Huanwu decoction administration, the order parameter S gradually decreased (P < 0.01). That is, cell membrane fluidity was restored to normal levels in a dose-dependent manner. The repair of membrane fluidity reflects the curative effect of Buyang Huanwu decoction. In the model group, the correlation coefficient between Buyang Huanwu decoction concentrations from 1 to 80 mg/mL and the order parameter S was 0.82 (P < 0.05; Figure 2), whereas in the sham surgery group, Buyang Huanwu decoction did not influence normal neuron function within the dose range.


Buyang Huanwu decoction enhances cell membrane fluidity in rats with cerebral ischemia/reperfusion.

Li C - Neural Regen Res (2012)

Correlation between Buyang Huanwu decoction (BYHWD) and the order parameter S in the model group (r = 0.82).Statistical analysis was performed by Pearson correlation analysis.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308761&req=5

Figure 2: Correlation between Buyang Huanwu decoction (BYHWD) and the order parameter S in the model group (r = 0.82).Statistical analysis was performed by Pearson correlation analysis.
Mentions: This indicated that cell membrane fluidity declined and cell function was affected in stroke model rats. After increasing doses of Buyang Huanwu decoction administration, the order parameter S gradually decreased (P < 0.01). That is, cell membrane fluidity was restored to normal levels in a dose-dependent manner. The repair of membrane fluidity reflects the curative effect of Buyang Huanwu decoction. In the model group, the correlation coefficient between Buyang Huanwu decoction concentrations from 1 to 80 mg/mL and the order parameter S was 0.82 (P < 0.05; Figure 2), whereas in the sham surgery group, Buyang Huanwu decoction did not influence normal neuron function within the dose range.

Bottom Line: After bilateral carotid artery occlusion for 30 minutes and reperfusion for 2 hours, distinct pathological changes presented in the cerebral cortex and cerebellum of rats.Compared with normal rats, nerve cell membrane fluidity significantly decreased in ischemia/reperfusion rats as detected by spin-labeling electron spin resonance, consistent with order parameter S and rotational correlation time τc measurements.Results showed that Buyang Huanwu decoction gradually increased membrane fluidity dose-dependently to normal levels, and eliminated hydroxide (OH(·)) and superoxide ( O2 (·)) free radicals dose-dependently.

View Article: PubMed Central - PubMed

Affiliation: Institute of Brain Sciences, Department of Physiology, Medical College, Datong University, Datong 037009, Shanxi Province, China.

ABSTRACT
After bilateral carotid artery occlusion for 30 minutes and reperfusion for 2 hours, distinct pathological changes presented in the cerebral cortex and cerebellum of rats. Compared with normal rats, nerve cell membrane fluidity significantly decreased in ischemia/reperfusion rats as detected by spin-labeling electron spin resonance, consistent with order parameter S and rotational correlation time τc measurements. Brain nerve cells from rats with ischemia/reperfusion injury were cultured with 1-100 mg/mL Buyang Huanwu decoction. Results showed that Buyang Huanwu decoction gradually increased membrane fluidity dose-dependently to normal levels, and eliminated hydroxide (OH(·)) and superoxide ( O2 (·)) free radicals dose-dependently. These findings suggest that Buyang Huanwu decoction can protect against cell membrane fluidity changes in rats with ischemia/ reperfusion injury by scavenging free radicals.

No MeSH data available.


Related in: MedlinePlus