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Buyang Huanwu decoction enhances cell membrane fluidity in rats with cerebral ischemia/reperfusion.

Li C - Neural Regen Res (2012)

Bottom Line: After bilateral carotid artery occlusion for 30 minutes and reperfusion for 2 hours, distinct pathological changes presented in the cerebral cortex and cerebellum of rats.Compared with normal rats, nerve cell membrane fluidity significantly decreased in ischemia/reperfusion rats as detected by spin-labeling electron spin resonance, consistent with order parameter S and rotational correlation time τc measurements.Results showed that Buyang Huanwu decoction gradually increased membrane fluidity dose-dependently to normal levels, and eliminated hydroxide (OH(·)) and superoxide ( O2 (·)) free radicals dose-dependently.

View Article: PubMed Central - PubMed

Affiliation: Institute of Brain Sciences, Department of Physiology, Medical College, Datong University, Datong 037009, Shanxi Province, China.

ABSTRACT
After bilateral carotid artery occlusion for 30 minutes and reperfusion for 2 hours, distinct pathological changes presented in the cerebral cortex and cerebellum of rats. Compared with normal rats, nerve cell membrane fluidity significantly decreased in ischemia/reperfusion rats as detected by spin-labeling electron spin resonance, consistent with order parameter S and rotational correlation time τc measurements. Brain nerve cells from rats with ischemia/reperfusion injury were cultured with 1-100 mg/mL Buyang Huanwu decoction. Results showed that Buyang Huanwu decoction gradually increased membrane fluidity dose-dependently to normal levels, and eliminated hydroxide (OH(·)) and superoxide ( O2 (·)) free radicals dose-dependently. These findings suggest that Buyang Huanwu decoction can protect against cell membrane fluidity changes in rats with ischemia/ reperfusion injury by scavenging free radicals.

No MeSH data available.


Related in: MedlinePlus

Morphology of brain tissue in rats (hematoxylin-eosin staining, × 400).In the cerebrum (A) and cerebellum (B) in sham surgery group, the configuration of neurons was clear, and there was no evidence of injury. The arrow shows a normal Purkinje's neuron.(C, D) In the cortex of model group, the configuration of neurons was not clear, and local hemorrhaging was observed.In the cerebellum (D), the metamorphosis of Purkinje neurons was observed (as shown by the arrow). Some Purkinje neurons were lost.
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Figure 1: Morphology of brain tissue in rats (hematoxylin-eosin staining, × 400).In the cerebrum (A) and cerebellum (B) in sham surgery group, the configuration of neurons was clear, and there was no evidence of injury. The arrow shows a normal Purkinje's neuron.(C, D) In the cortex of model group, the configuration of neurons was not clear, and local hemorrhaging was observed.In the cerebellum (D), the metamorphosis of Purkinje neurons was observed (as shown by the arrow). Some Purkinje neurons were lost.

Mentions: Results from hematoxylin-eosin staining showed no evidence of brain injury in the sham surgery group. Brain tissue slices were harvested from the cortex of the frontal lobe of the cerebrum as well as the cerebellum[14]. The structures of neurons were clear, and nucleoli were clearly visible. In the model group, tissue slices presented pathological changes similar to that in humans following a stroke, such as local hemorrhaging and neuronal deep dyeing in the cortex of the frontal lobe. Swelling, denaturation, necrosis or loss of neurons could be observed near the hemorrhage. In the cerebellum, metamorphosis and necrosis of Purkinje neurons was observed, and nuclei were not visible. Some Purkinje's neurons were even lost (Figure 1).


Buyang Huanwu decoction enhances cell membrane fluidity in rats with cerebral ischemia/reperfusion.

Li C - Neural Regen Res (2012)

Morphology of brain tissue in rats (hematoxylin-eosin staining, × 400).In the cerebrum (A) and cerebellum (B) in sham surgery group, the configuration of neurons was clear, and there was no evidence of injury. The arrow shows a normal Purkinje's neuron.(C, D) In the cortex of model group, the configuration of neurons was not clear, and local hemorrhaging was observed.In the cerebellum (D), the metamorphosis of Purkinje neurons was observed (as shown by the arrow). Some Purkinje neurons were lost.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308761&req=5

Figure 1: Morphology of brain tissue in rats (hematoxylin-eosin staining, × 400).In the cerebrum (A) and cerebellum (B) in sham surgery group, the configuration of neurons was clear, and there was no evidence of injury. The arrow shows a normal Purkinje's neuron.(C, D) In the cortex of model group, the configuration of neurons was not clear, and local hemorrhaging was observed.In the cerebellum (D), the metamorphosis of Purkinje neurons was observed (as shown by the arrow). Some Purkinje neurons were lost.
Mentions: Results from hematoxylin-eosin staining showed no evidence of brain injury in the sham surgery group. Brain tissue slices were harvested from the cortex of the frontal lobe of the cerebrum as well as the cerebellum[14]. The structures of neurons were clear, and nucleoli were clearly visible. In the model group, tissue slices presented pathological changes similar to that in humans following a stroke, such as local hemorrhaging and neuronal deep dyeing in the cortex of the frontal lobe. Swelling, denaturation, necrosis or loss of neurons could be observed near the hemorrhage. In the cerebellum, metamorphosis and necrosis of Purkinje neurons was observed, and nuclei were not visible. Some Purkinje's neurons were even lost (Figure 1).

Bottom Line: After bilateral carotid artery occlusion for 30 minutes and reperfusion for 2 hours, distinct pathological changes presented in the cerebral cortex and cerebellum of rats.Compared with normal rats, nerve cell membrane fluidity significantly decreased in ischemia/reperfusion rats as detected by spin-labeling electron spin resonance, consistent with order parameter S and rotational correlation time τc measurements.Results showed that Buyang Huanwu decoction gradually increased membrane fluidity dose-dependently to normal levels, and eliminated hydroxide (OH(·)) and superoxide ( O2 (·)) free radicals dose-dependently.

View Article: PubMed Central - PubMed

Affiliation: Institute of Brain Sciences, Department of Physiology, Medical College, Datong University, Datong 037009, Shanxi Province, China.

ABSTRACT
After bilateral carotid artery occlusion for 30 minutes and reperfusion for 2 hours, distinct pathological changes presented in the cerebral cortex and cerebellum of rats. Compared with normal rats, nerve cell membrane fluidity significantly decreased in ischemia/reperfusion rats as detected by spin-labeling electron spin resonance, consistent with order parameter S and rotational correlation time τc measurements. Brain nerve cells from rats with ischemia/reperfusion injury were cultured with 1-100 mg/mL Buyang Huanwu decoction. Results showed that Buyang Huanwu decoction gradually increased membrane fluidity dose-dependently to normal levels, and eliminated hydroxide (OH(·)) and superoxide ( O2 (·)) free radicals dose-dependently. These findings suggest that Buyang Huanwu decoction can protect against cell membrane fluidity changes in rats with ischemia/ reperfusion injury by scavenging free radicals.

No MeSH data available.


Related in: MedlinePlus