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Nav1.7 protein and mRNA expression in the dorsal root ganglia of rats with chronic neuropathic pain.

Liu C, Cao J, Ren X, Zang W - Neural Regen Res (2012)

Bottom Line: Behavioral tests showed that the thresholds for thermal and mechanical hyperalgesia were significantly reduced in neuropathic pain rats 3-28 days following model induction.The results of immunohistochemistry, western blot assays and reverse transcription-PCR showed that Nav1.7 protein and mRNA expression was significantly increased in the injured dorsal root ganglia.These findings indicated that Nav1.7 might play an important role in the model of chronic neuropathic pain.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Anatomy, Department of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, Henan Province, China.

ABSTRACT
Neuropathic pain was produced by chronic constriction injury of the sciatic nerve in rats. Behavioral tests showed that the thresholds for thermal and mechanical hyperalgesia were significantly reduced in neuropathic pain rats 3-28 days following model induction. The results of immunohistochemistry, western blot assays and reverse transcription-PCR showed that Nav1.7 protein and mRNA expression was significantly increased in the injured dorsal root ganglia. These findings indicated that Nav1.7 might play an important role in the model of chronic neuropathic pain.

No MeSH data available.


Related in: MedlinePlus

Expression of Nav1.7 mRNA in the injured dorsal root ganglion.(A) Electrophoresis of Nav1.7 mRNA. M: Marker 2000; 1: control; 2: sham surgery 1 day; 3: chronic constriction injury (CCI) 1 day; 4: sham surgery 7 days; 5: CCI 7 days; 6: sham surgery 28 days; 7: CCI 28 days.(B) Quantification of Nav1.7 mRNA expression. Compared with the control group, expression of CCI-induced Nav1.7 mRNA was significantly increased in the injured dorsal root ganglion. The Nav1.7/β-actin ratio in the control group was set to 100%. Data are expressed as mean ± SEM of three rats in each group. aP < 0.05, vs. control and sham surgery groups (Student's t-test).
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Figure 4: Expression of Nav1.7 mRNA in the injured dorsal root ganglion.(A) Electrophoresis of Nav1.7 mRNA. M: Marker 2000; 1: control; 2: sham surgery 1 day; 3: chronic constriction injury (CCI) 1 day; 4: sham surgery 7 days; 5: CCI 7 days; 6: sham surgery 28 days; 7: CCI 28 days.(B) Quantification of Nav1.7 mRNA expression. Compared with the control group, expression of CCI-induced Nav1.7 mRNA was significantly increased in the injured dorsal root ganglion. The Nav1.7/β-actin ratio in the control group was set to 100%. Data are expressed as mean ± SEM of three rats in each group. aP < 0.05, vs. control and sham surgery groups (Student's t-test).

Mentions: RT-PCR showed that, compared with the sham surgery and control groups, Nav1.7 mRNA expression levels were significantly increased in the injured dorsal root ganglion of the CCI group (P < 0.05; Figure 4).


Nav1.7 protein and mRNA expression in the dorsal root ganglia of rats with chronic neuropathic pain.

Liu C, Cao J, Ren X, Zang W - Neural Regen Res (2012)

Expression of Nav1.7 mRNA in the injured dorsal root ganglion.(A) Electrophoresis of Nav1.7 mRNA. M: Marker 2000; 1: control; 2: sham surgery 1 day; 3: chronic constriction injury (CCI) 1 day; 4: sham surgery 7 days; 5: CCI 7 days; 6: sham surgery 28 days; 7: CCI 28 days.(B) Quantification of Nav1.7 mRNA expression. Compared with the control group, expression of CCI-induced Nav1.7 mRNA was significantly increased in the injured dorsal root ganglion. The Nav1.7/β-actin ratio in the control group was set to 100%. Data are expressed as mean ± SEM of three rats in each group. aP < 0.05, vs. control and sham surgery groups (Student's t-test).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308749&req=5

Figure 4: Expression of Nav1.7 mRNA in the injured dorsal root ganglion.(A) Electrophoresis of Nav1.7 mRNA. M: Marker 2000; 1: control; 2: sham surgery 1 day; 3: chronic constriction injury (CCI) 1 day; 4: sham surgery 7 days; 5: CCI 7 days; 6: sham surgery 28 days; 7: CCI 28 days.(B) Quantification of Nav1.7 mRNA expression. Compared with the control group, expression of CCI-induced Nav1.7 mRNA was significantly increased in the injured dorsal root ganglion. The Nav1.7/β-actin ratio in the control group was set to 100%. Data are expressed as mean ± SEM of three rats in each group. aP < 0.05, vs. control and sham surgery groups (Student's t-test).
Mentions: RT-PCR showed that, compared with the sham surgery and control groups, Nav1.7 mRNA expression levels were significantly increased in the injured dorsal root ganglion of the CCI group (P < 0.05; Figure 4).

Bottom Line: Behavioral tests showed that the thresholds for thermal and mechanical hyperalgesia were significantly reduced in neuropathic pain rats 3-28 days following model induction.The results of immunohistochemistry, western blot assays and reverse transcription-PCR showed that Nav1.7 protein and mRNA expression was significantly increased in the injured dorsal root ganglia.These findings indicated that Nav1.7 might play an important role in the model of chronic neuropathic pain.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Anatomy, Department of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, Henan Province, China.

ABSTRACT
Neuropathic pain was produced by chronic constriction injury of the sciatic nerve in rats. Behavioral tests showed that the thresholds for thermal and mechanical hyperalgesia were significantly reduced in neuropathic pain rats 3-28 days following model induction. The results of immunohistochemistry, western blot assays and reverse transcription-PCR showed that Nav1.7 protein and mRNA expression was significantly increased in the injured dorsal root ganglia. These findings indicated that Nav1.7 might play an important role in the model of chronic neuropathic pain.

No MeSH data available.


Related in: MedlinePlus