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Differential protein expression in spinal cord tissue of a rabbit model of spinal cord ischemia/reperfusion injury.

Gao Q, Liang Y, Yang X, Liu G, Li X, Zhu B, Liu J, Yang M, Xia W, Dong J, Jiao J - Neural Regen Res (2012)

Bottom Line: In the ischemia group, the expression of eight proteins was up regulated, and that of the remaining four proteins was down regulated.In the sham-surgery group, only one protein was detected.These proteins were related to energy metabolism, cell defense, inflammatory mechanism and cell signaling.

View Article: PubMed Central - PubMed

Affiliation: Team of Spine and Spinal Cord, Department of Orthopedics, China-Japan Friendship Hospital, Jilin University, Changchun 130033, Jilin Province, China.

ABSTRACT
New Zealand rabbits were randomly divided into an ischemia group (occlusion of the abdominal aorta for 60 minutes), an ischemia-reperfusion group (occlusion of the abdominal aorta for 60 minutes followed by 48 hours of reperfusion) and a sham-surgery group. Two-dimensional gel electrophoresis detected 49 differentially expressed proteins in spinal cord tissue from the ischemia and ischemia/ reperfusion groups and 23 of them were identified by mass spectrometry. In the ischemia group, the expression of eight proteins was up regulated, and that of the remaining four proteins was down regulated. In the ischemia/reperfusion group, the expression of four proteins was up regulated, and that of two proteins was down regulated. In the sham-surgery group, only one protein was detected. In the ischemia and ischemia/reperfusion groups, four proteins overlapped between groups with the same differential expression, including three that were up regulated and one down regulated. These proteins were related to energy metabolism, cell defense, inflammatory mechanism and cell signaling.

No MeSH data available.


Related in: MedlinePlus

Fluorescent two-dimensional gel electrophoretogram of sham-surgery, ischemia, and ischemia/reperfusion groups.Compared with sham-surgery group (A, C), there were 49 differentially expressed protein spots in the ischemia (B) group and ischemia/reperfusion group (D).
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Figure 1: Fluorescent two-dimensional gel electrophoretogram of sham-surgery, ischemia, and ischemia/reperfusion groups.Compared with sham-surgery group (A, C), there were 49 differentially expressed protein spots in the ischemia (B) group and ischemia/reperfusion group (D).

Mentions: Spinal cord tissue proteins were separated using CyDye DIGE Fluor saturation dye-labeled two-dimensional gel electrophoresis and study of differentially expressed proteins was achieved by image analysis using DeCyder software system. Using the spinal cord protein expression spectrum from the sham-surgery group as a standard, protein expression spectra at two different time points, i.e., ischemia for 60 minutes and ischemia for 60 minutes followed by 48 hours of reperfusion were established (Figure 1). Protein spots from the ischemia group and ischemia/reperfusion group with a 2-fold expression difference compared to the sham surgery group, were included for further analysis. In total, 49 protein spots with obvious differential expression were screened. Compared with the sham-surgery group, 25 proteins were up regulated and five proteins were down regulated in the ischemia group, and 16 proteins were up regulated and three proteins were down regulated in the ischemia/reperfusion group.


Differential protein expression in spinal cord tissue of a rabbit model of spinal cord ischemia/reperfusion injury.

Gao Q, Liang Y, Yang X, Liu G, Li X, Zhu B, Liu J, Yang M, Xia W, Dong J, Jiao J - Neural Regen Res (2012)

Fluorescent two-dimensional gel electrophoretogram of sham-surgery, ischemia, and ischemia/reperfusion groups.Compared with sham-surgery group (A, C), there were 49 differentially expressed protein spots in the ischemia (B) group and ischemia/reperfusion group (D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308748&req=5

Figure 1: Fluorescent two-dimensional gel electrophoretogram of sham-surgery, ischemia, and ischemia/reperfusion groups.Compared with sham-surgery group (A, C), there were 49 differentially expressed protein spots in the ischemia (B) group and ischemia/reperfusion group (D).
Mentions: Spinal cord tissue proteins were separated using CyDye DIGE Fluor saturation dye-labeled two-dimensional gel electrophoresis and study of differentially expressed proteins was achieved by image analysis using DeCyder software system. Using the spinal cord protein expression spectrum from the sham-surgery group as a standard, protein expression spectra at two different time points, i.e., ischemia for 60 minutes and ischemia for 60 minutes followed by 48 hours of reperfusion were established (Figure 1). Protein spots from the ischemia group and ischemia/reperfusion group with a 2-fold expression difference compared to the sham surgery group, were included for further analysis. In total, 49 protein spots with obvious differential expression were screened. Compared with the sham-surgery group, 25 proteins were up regulated and five proteins were down regulated in the ischemia group, and 16 proteins were up regulated and three proteins were down regulated in the ischemia/reperfusion group.

Bottom Line: In the ischemia group, the expression of eight proteins was up regulated, and that of the remaining four proteins was down regulated.In the sham-surgery group, only one protein was detected.These proteins were related to energy metabolism, cell defense, inflammatory mechanism and cell signaling.

View Article: PubMed Central - PubMed

Affiliation: Team of Spine and Spinal Cord, Department of Orthopedics, China-Japan Friendship Hospital, Jilin University, Changchun 130033, Jilin Province, China.

ABSTRACT
New Zealand rabbits were randomly divided into an ischemia group (occlusion of the abdominal aorta for 60 minutes), an ischemia-reperfusion group (occlusion of the abdominal aorta for 60 minutes followed by 48 hours of reperfusion) and a sham-surgery group. Two-dimensional gel electrophoresis detected 49 differentially expressed proteins in spinal cord tissue from the ischemia and ischemia/ reperfusion groups and 23 of them were identified by mass spectrometry. In the ischemia group, the expression of eight proteins was up regulated, and that of the remaining four proteins was down regulated. In the ischemia/reperfusion group, the expression of four proteins was up regulated, and that of two proteins was down regulated. In the sham-surgery group, only one protein was detected. In the ischemia and ischemia/reperfusion groups, four proteins overlapped between groups with the same differential expression, including three that were up regulated and one down regulated. These proteins were related to energy metabolism, cell defense, inflammatory mechanism and cell signaling.

No MeSH data available.


Related in: MedlinePlus