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The impact of resveratrol and hydrogen peroxide on muscle cell plasticity shows a dose-dependent interaction.

Bosutti A, Degens H - Sci Rep (2015)

Bottom Line: RS did not increase oxidative capacity.In conclusion, low resveratrol doses promoted in vitro muscle regeneration and attenuated the impact of ROS, while high doses augmented the reduced plasticity and metabolism induced by oxidative stress.Thus, the effects of resveratrol depend on its dose and degree of oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: School of Healthcare Science, Manchester Metropolitan University, Manchester, United Kingdom.

ABSTRACT
While reactive oxygen species (ROS) play a role in muscle repair, excessive amounts of ROS for extended periods may lead to oxidative stress. Antioxidants, as resveratrol (RS), may reduce oxidative stress, restore mitochondrial function and promote myogenesis and hypertrophy. However, RS dose-effectiveness for muscle plasticity is unclear. Therefore, we investigated RS dose-response on C2C12 myoblast and myotube plasticity 1. in the presence and 2. absence of different degrees of oxidative stress. Low RS concentration (10 μM) stimulated myoblast cell cycle arrest, migration and sprouting, which were inhibited by higher doses (40-60 μM). RS did not increase oxidative capacity. In contrast, RS induced mitochondria loss, reduced cell viability and ROS production, and activated stress response pathways [Hsp70 and pSer36-p66(ShcA) proteins]. However, the deleterious effects of H2O2 (1000 µM) on cell migration were alleviated after preconditioning with 10 µM-RS. This dose also enhanced cell motility mediated by 100 µM-H2O2, while higher RS-doses augmented the H2O2-induced impaired myoblast regeneration and mitochondrial dehydrogenase activity. In conclusion, low resveratrol doses promoted in vitro muscle regeneration and attenuated the impact of ROS, while high doses augmented the reduced plasticity and metabolism induced by oxidative stress. Thus, the effects of resveratrol depend on its dose and degree of oxidative stress.

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Related in: MedlinePlus

Resveratrol modulates myosin type 1 and total myosin ATPase activity.Representative phase contrast images showing the effect of 24 h (a) and 48 h (b) of 10, 20, 40 and 60 µM resveratrol (RS) on intracellular myosin type 1 and total myosin (type 1 plus type 2) ATPase activity in C2C12 myotubes after 8 days in differentiation media (2% FBS). RS 10–20 µM did not significantly affect myosin ATPase activity either after 24 h (ai;aii) or 48 h (bi;bii). Forty and 60 µM RS caused a transient decrease in both type 1 (60 µM) and total (40 and 60 µM) myosin ATPase after 24 h incubation (ai;aii) and an increase in total myosin (bii), but not type 1 myosin (above normal levels) after 48 h incubation. P-values calculated using a two-tailed Student's t-test.*: P<0.01 vs CT; ¤: P<0.05 vs CT. DM vs. CT: in none of the cases significant. Bars 20 µm. Original magnification 100×. Data are expressed as mean ± s.e.m. of biological triplicates.
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f4: Resveratrol modulates myosin type 1 and total myosin ATPase activity.Representative phase contrast images showing the effect of 24 h (a) and 48 h (b) of 10, 20, 40 and 60 µM resveratrol (RS) on intracellular myosin type 1 and total myosin (type 1 plus type 2) ATPase activity in C2C12 myotubes after 8 days in differentiation media (2% FBS). RS 10–20 µM did not significantly affect myosin ATPase activity either after 24 h (ai;aii) or 48 h (bi;bii). Forty and 60 µM RS caused a transient decrease in both type 1 (60 µM) and total (40 and 60 µM) myosin ATPase after 24 h incubation (ai;aii) and an increase in total myosin (bii), but not type 1 myosin (above normal levels) after 48 h incubation. P-values calculated using a two-tailed Student's t-test.*: P<0.01 vs CT; ¤: P<0.05 vs CT. DM vs. CT: in none of the cases significant. Bars 20 µm. Original magnification 100×. Data are expressed as mean ± s.e.m. of biological triplicates.

Mentions: To gain more information about the effect of RS on the properties of myofibrils, we tested the effect of different concentrations of RS on myosin type 1- and total myosin ATPase activities in C2C12 myotubes (Fig. 4). The effects of RS (10–60 µM) were followed for 24 h (Fig. 4a) and 48 h (Fig. 4b) to establish the dose- and time-dependent response to the treatment.


The impact of resveratrol and hydrogen peroxide on muscle cell plasticity shows a dose-dependent interaction.

Bosutti A, Degens H - Sci Rep (2015)

Resveratrol modulates myosin type 1 and total myosin ATPase activity.Representative phase contrast images showing the effect of 24 h (a) and 48 h (b) of 10, 20, 40 and 60 µM resveratrol (RS) on intracellular myosin type 1 and total myosin (type 1 plus type 2) ATPase activity in C2C12 myotubes after 8 days in differentiation media (2% FBS). RS 10–20 µM did not significantly affect myosin ATPase activity either after 24 h (ai;aii) or 48 h (bi;bii). Forty and 60 µM RS caused a transient decrease in both type 1 (60 µM) and total (40 and 60 µM) myosin ATPase after 24 h incubation (ai;aii) and an increase in total myosin (bii), but not type 1 myosin (above normal levels) after 48 h incubation. P-values calculated using a two-tailed Student's t-test.*: P<0.01 vs CT; ¤: P<0.05 vs CT. DM vs. CT: in none of the cases significant. Bars 20 µm. Original magnification 100×. Data are expressed as mean ± s.e.m. of biological triplicates.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4308712&req=5

f4: Resveratrol modulates myosin type 1 and total myosin ATPase activity.Representative phase contrast images showing the effect of 24 h (a) and 48 h (b) of 10, 20, 40 and 60 µM resveratrol (RS) on intracellular myosin type 1 and total myosin (type 1 plus type 2) ATPase activity in C2C12 myotubes after 8 days in differentiation media (2% FBS). RS 10–20 µM did not significantly affect myosin ATPase activity either after 24 h (ai;aii) or 48 h (bi;bii). Forty and 60 µM RS caused a transient decrease in both type 1 (60 µM) and total (40 and 60 µM) myosin ATPase after 24 h incubation (ai;aii) and an increase in total myosin (bii), but not type 1 myosin (above normal levels) after 48 h incubation. P-values calculated using a two-tailed Student's t-test.*: P<0.01 vs CT; ¤: P<0.05 vs CT. DM vs. CT: in none of the cases significant. Bars 20 µm. Original magnification 100×. Data are expressed as mean ± s.e.m. of biological triplicates.
Mentions: To gain more information about the effect of RS on the properties of myofibrils, we tested the effect of different concentrations of RS on myosin type 1- and total myosin ATPase activities in C2C12 myotubes (Fig. 4). The effects of RS (10–60 µM) were followed for 24 h (Fig. 4a) and 48 h (Fig. 4b) to establish the dose- and time-dependent response to the treatment.

Bottom Line: RS did not increase oxidative capacity.In conclusion, low resveratrol doses promoted in vitro muscle regeneration and attenuated the impact of ROS, while high doses augmented the reduced plasticity and metabolism induced by oxidative stress.Thus, the effects of resveratrol depend on its dose and degree of oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: School of Healthcare Science, Manchester Metropolitan University, Manchester, United Kingdom.

ABSTRACT
While reactive oxygen species (ROS) play a role in muscle repair, excessive amounts of ROS for extended periods may lead to oxidative stress. Antioxidants, as resveratrol (RS), may reduce oxidative stress, restore mitochondrial function and promote myogenesis and hypertrophy. However, RS dose-effectiveness for muscle plasticity is unclear. Therefore, we investigated RS dose-response on C2C12 myoblast and myotube plasticity 1. in the presence and 2. absence of different degrees of oxidative stress. Low RS concentration (10 μM) stimulated myoblast cell cycle arrest, migration and sprouting, which were inhibited by higher doses (40-60 μM). RS did not increase oxidative capacity. In contrast, RS induced mitochondria loss, reduced cell viability and ROS production, and activated stress response pathways [Hsp70 and pSer36-p66(ShcA) proteins]. However, the deleterious effects of H2O2 (1000 µM) on cell migration were alleviated after preconditioning with 10 µM-RS. This dose also enhanced cell motility mediated by 100 µM-H2O2, while higher RS-doses augmented the H2O2-induced impaired myoblast regeneration and mitochondrial dehydrogenase activity. In conclusion, low resveratrol doses promoted in vitro muscle regeneration and attenuated the impact of ROS, while high doses augmented the reduced plasticity and metabolism induced by oxidative stress. Thus, the effects of resveratrol depend on its dose and degree of oxidative stress.

Show MeSH
Related in: MedlinePlus