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Antibiotic resistance and prevalence of class 1 and 2 integrons in Escherichia coli isolated from two wastewater treatment plants, and their receiving waters (Gulf of Gdansk, Baltic Sea, Poland).

Kotlarska E, Łuczkiewicz A, Pisowacka M, Burzyński A - Environ Sci Pollut Res Int (2014)

Bottom Line: The presence of integrons was associated with increased frequency of resistance to fluoroquinolones, trimethoprim/sulfamethoxazole, amoxicillin/clavulanate, piperacillin/tazobactam, and presence of multidrug-resistance phenotype.Nine different gene cassette arrays were confirmed among sequenced variable regions, with predominance of dfrA1-aadA1, dfrA17-aadA5, and aadA1 arrays.These findings illustrate the importance of WWTPs in spreading of resistance genes in the environment and the need for inclusion of at least monitoring efforts in the regular WWTP processes.

View Article: PubMed Central - PubMed

Affiliation: Genetics and Marine Biotechnology Department, Institute of Oceanology of the Polish Academy of Sciences, Powstancow Warszawy 55, 81-712, Sopot, Poland, ewachec@iopan.gda.pl.

ABSTRACT
In this study, antimicrobial-resistance patterns were analyzed in Escherichia coli isolates from raw (RW) and treated wastewater (TW) of two wastewater treatment plants (WWTPs), their marine outfalls (MOut), and mouth of the Vistula River (VR). Susceptibility of E. coli was tested against different classes of antibiotics. Isolates resistant to at least one antimicrobial agent were PCR tested for the presence of integrons. Ampicillin-resistant E. coli were the most frequent, followed by amoxicillin/clavulanate (up to 32 %), trimethoprim/sulfamethoxazole (up to 20 %), and fluoroquinolone (up to 15 %)-resistant isolates. Presence of class 1 and 2 integrons was detected among tested E. coli isolates with rate of 32.06 % (n = 84) and 3.05 % (n = 8), respectively. The presence of integrons was associated with increased frequency of resistance to fluoroquinolones, trimethoprim/sulfamethoxazole, amoxicillin/clavulanate, piperacillin/tazobactam, and presence of multidrug-resistance phenotype. Variable regions were detected in 48 class 1 and 5 class 2 integron-positive isolates. Nine different gene cassette arrays were confirmed among sequenced variable regions, with predominance of dfrA1-aadA1, dfrA17-aadA5, and aadA1 arrays. These findings illustrate the importance of WWTPs in spreading of resistance genes in the environment and the need for inclusion of at least monitoring efforts in the regular WWTP processes.

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Susceptibility of E. coli isolates to increasing number of antimicrobial agents (S sensitive to all, R1-R ≥ 5 resistant from 1 to 5 and more antimicrobial agents)
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Related In: Results  -  Collection


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Fig3: Susceptibility of E. coli isolates to increasing number of antimicrobial agents (S sensitive to all, R1-R ≥ 5 resistant from 1 to 5 and more antimicrobial agents)

Mentions: Among 37 and 34 % of E. coli isolated from raw wastewater samples (WRW and DRW, respectively), resistance to at least one of the antimicrobial agents was noted (Fig. 3). In both WWTP effluents, the resistance rate increased to 44 % for WTW and 38 % for DTW. In the case of marine outfalls rising, trends were observed only for WMOut (47 % of E. coli isolates showed resistance), while resistance rate observed in DMOut was significantly lower (18 %) and similar to that noted for mouth of the VR (23 %).Fig. 3


Antibiotic resistance and prevalence of class 1 and 2 integrons in Escherichia coli isolated from two wastewater treatment plants, and their receiving waters (Gulf of Gdansk, Baltic Sea, Poland).

Kotlarska E, Łuczkiewicz A, Pisowacka M, Burzyński A - Environ Sci Pollut Res Int (2014)

Susceptibility of E. coli isolates to increasing number of antimicrobial agents (S sensitive to all, R1-R ≥ 5 resistant from 1 to 5 and more antimicrobial agents)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4308648&req=5

Fig3: Susceptibility of E. coli isolates to increasing number of antimicrobial agents (S sensitive to all, R1-R ≥ 5 resistant from 1 to 5 and more antimicrobial agents)
Mentions: Among 37 and 34 % of E. coli isolated from raw wastewater samples (WRW and DRW, respectively), resistance to at least one of the antimicrobial agents was noted (Fig. 3). In both WWTP effluents, the resistance rate increased to 44 % for WTW and 38 % for DTW. In the case of marine outfalls rising, trends were observed only for WMOut (47 % of E. coli isolates showed resistance), while resistance rate observed in DMOut was significantly lower (18 %) and similar to that noted for mouth of the VR (23 %).Fig. 3

Bottom Line: The presence of integrons was associated with increased frequency of resistance to fluoroquinolones, trimethoprim/sulfamethoxazole, amoxicillin/clavulanate, piperacillin/tazobactam, and presence of multidrug-resistance phenotype.Nine different gene cassette arrays were confirmed among sequenced variable regions, with predominance of dfrA1-aadA1, dfrA17-aadA5, and aadA1 arrays.These findings illustrate the importance of WWTPs in spreading of resistance genes in the environment and the need for inclusion of at least monitoring efforts in the regular WWTP processes.

View Article: PubMed Central - PubMed

Affiliation: Genetics and Marine Biotechnology Department, Institute of Oceanology of the Polish Academy of Sciences, Powstancow Warszawy 55, 81-712, Sopot, Poland, ewachec@iopan.gda.pl.

ABSTRACT
In this study, antimicrobial-resistance patterns were analyzed in Escherichia coli isolates from raw (RW) and treated wastewater (TW) of two wastewater treatment plants (WWTPs), their marine outfalls (MOut), and mouth of the Vistula River (VR). Susceptibility of E. coli was tested against different classes of antibiotics. Isolates resistant to at least one antimicrobial agent were PCR tested for the presence of integrons. Ampicillin-resistant E. coli were the most frequent, followed by amoxicillin/clavulanate (up to 32 %), trimethoprim/sulfamethoxazole (up to 20 %), and fluoroquinolone (up to 15 %)-resistant isolates. Presence of class 1 and 2 integrons was detected among tested E. coli isolates with rate of 32.06 % (n = 84) and 3.05 % (n = 8), respectively. The presence of integrons was associated with increased frequency of resistance to fluoroquinolones, trimethoprim/sulfamethoxazole, amoxicillin/clavulanate, piperacillin/tazobactam, and presence of multidrug-resistance phenotype. Variable regions were detected in 48 class 1 and 5 class 2 integron-positive isolates. Nine different gene cassette arrays were confirmed among sequenced variable regions, with predominance of dfrA1-aadA1, dfrA17-aadA5, and aadA1 arrays. These findings illustrate the importance of WWTPs in spreading of resistance genes in the environment and the need for inclusion of at least monitoring efforts in the regular WWTP processes.

Show MeSH
Related in: MedlinePlus