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Antibiotic resistance and prevalence of class 1 and 2 integrons in Escherichia coli isolated from two wastewater treatment plants, and their receiving waters (Gulf of Gdansk, Baltic Sea, Poland).

Kotlarska E, Łuczkiewicz A, Pisowacka M, Burzyński A - Environ Sci Pollut Res Int (2014)

Bottom Line: The presence of integrons was associated with increased frequency of resistance to fluoroquinolones, trimethoprim/sulfamethoxazole, amoxicillin/clavulanate, piperacillin/tazobactam, and presence of multidrug-resistance phenotype.Nine different gene cassette arrays were confirmed among sequenced variable regions, with predominance of dfrA1-aadA1, dfrA17-aadA5, and aadA1 arrays.These findings illustrate the importance of WWTPs in spreading of resistance genes in the environment and the need for inclusion of at least monitoring efforts in the regular WWTP processes.

View Article: PubMed Central - PubMed

Affiliation: Genetics and Marine Biotechnology Department, Institute of Oceanology of the Polish Academy of Sciences, Powstancow Warszawy 55, 81-712, Sopot, Poland, ewachec@iopan.gda.pl.

ABSTRACT
In this study, antimicrobial-resistance patterns were analyzed in Escherichia coli isolates from raw (RW) and treated wastewater (TW) of two wastewater treatment plants (WWTPs), their marine outfalls (MOut), and mouth of the Vistula River (VR). Susceptibility of E. coli was tested against different classes of antibiotics. Isolates resistant to at least one antimicrobial agent were PCR tested for the presence of integrons. Ampicillin-resistant E. coli were the most frequent, followed by amoxicillin/clavulanate (up to 32 %), trimethoprim/sulfamethoxazole (up to 20 %), and fluoroquinolone (up to 15 %)-resistant isolates. Presence of class 1 and 2 integrons was detected among tested E. coli isolates with rate of 32.06 % (n = 84) and 3.05 % (n = 8), respectively. The presence of integrons was associated with increased frequency of resistance to fluoroquinolones, trimethoprim/sulfamethoxazole, amoxicillin/clavulanate, piperacillin/tazobactam, and presence of multidrug-resistance phenotype. Variable regions were detected in 48 class 1 and 5 class 2 integron-positive isolates. Nine different gene cassette arrays were confirmed among sequenced variable regions, with predominance of dfrA1-aadA1, dfrA17-aadA5, and aadA1 arrays. These findings illustrate the importance of WWTPs in spreading of resistance genes in the environment and the need for inclusion of at least monitoring efforts in the regular WWTP processes.

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Sampling sites: raw (WRW) and treated (WTW) wastewater, as well as marine outfall (WMOut) of WWTP Gdansk–Wschod; raw (DRW) and treated (DTW) wastewater, as well as marine outfall (DMOut) of WWTP Gdynia–Debogorze. VR mouth of the Vistula River
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Fig1: Sampling sites: raw (WRW) and treated (WTW) wastewater, as well as marine outfall (WMOut) of WWTP Gdansk–Wschod; raw (DRW) and treated (DTW) wastewater, as well as marine outfall (DMOut) of WWTP Gdynia–Debogorze. VR mouth of the Vistula River

Mentions: Altogether, 36 samples were analyzed. Samples of raw and treated wastewater (RW and TW, respectively) were taken from two local wastewater treatment plants (WWTPs) and from their marine outfalls (MOut) (Fig. 1). In both cases, treated wastewater is discharged by submarine collectors, about 2.5 km long and equipped with diffuser systems. The plants mainly treat municipal wastewater. Industrial wastewater and non-disinfected hospital wastewater consist about 10 and 0.2 % of their daily inflow, respectively.Fig. 1


Antibiotic resistance and prevalence of class 1 and 2 integrons in Escherichia coli isolated from two wastewater treatment plants, and their receiving waters (Gulf of Gdansk, Baltic Sea, Poland).

Kotlarska E, Łuczkiewicz A, Pisowacka M, Burzyński A - Environ Sci Pollut Res Int (2014)

Sampling sites: raw (WRW) and treated (WTW) wastewater, as well as marine outfall (WMOut) of WWTP Gdansk–Wschod; raw (DRW) and treated (DTW) wastewater, as well as marine outfall (DMOut) of WWTP Gdynia–Debogorze. VR mouth of the Vistula River
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4308648&req=5

Fig1: Sampling sites: raw (WRW) and treated (WTW) wastewater, as well as marine outfall (WMOut) of WWTP Gdansk–Wschod; raw (DRW) and treated (DTW) wastewater, as well as marine outfall (DMOut) of WWTP Gdynia–Debogorze. VR mouth of the Vistula River
Mentions: Altogether, 36 samples were analyzed. Samples of raw and treated wastewater (RW and TW, respectively) were taken from two local wastewater treatment plants (WWTPs) and from their marine outfalls (MOut) (Fig. 1). In both cases, treated wastewater is discharged by submarine collectors, about 2.5 km long and equipped with diffuser systems. The plants mainly treat municipal wastewater. Industrial wastewater and non-disinfected hospital wastewater consist about 10 and 0.2 % of their daily inflow, respectively.Fig. 1

Bottom Line: The presence of integrons was associated with increased frequency of resistance to fluoroquinolones, trimethoprim/sulfamethoxazole, amoxicillin/clavulanate, piperacillin/tazobactam, and presence of multidrug-resistance phenotype.Nine different gene cassette arrays were confirmed among sequenced variable regions, with predominance of dfrA1-aadA1, dfrA17-aadA5, and aadA1 arrays.These findings illustrate the importance of WWTPs in spreading of resistance genes in the environment and the need for inclusion of at least monitoring efforts in the regular WWTP processes.

View Article: PubMed Central - PubMed

Affiliation: Genetics and Marine Biotechnology Department, Institute of Oceanology of the Polish Academy of Sciences, Powstancow Warszawy 55, 81-712, Sopot, Poland, ewachec@iopan.gda.pl.

ABSTRACT
In this study, antimicrobial-resistance patterns were analyzed in Escherichia coli isolates from raw (RW) and treated wastewater (TW) of two wastewater treatment plants (WWTPs), their marine outfalls (MOut), and mouth of the Vistula River (VR). Susceptibility of E. coli was tested against different classes of antibiotics. Isolates resistant to at least one antimicrobial agent were PCR tested for the presence of integrons. Ampicillin-resistant E. coli were the most frequent, followed by amoxicillin/clavulanate (up to 32 %), trimethoprim/sulfamethoxazole (up to 20 %), and fluoroquinolone (up to 15 %)-resistant isolates. Presence of class 1 and 2 integrons was detected among tested E. coli isolates with rate of 32.06 % (n = 84) and 3.05 % (n = 8), respectively. The presence of integrons was associated with increased frequency of resistance to fluoroquinolones, trimethoprim/sulfamethoxazole, amoxicillin/clavulanate, piperacillin/tazobactam, and presence of multidrug-resistance phenotype. Variable regions were detected in 48 class 1 and 5 class 2 integron-positive isolates. Nine different gene cassette arrays were confirmed among sequenced variable regions, with predominance of dfrA1-aadA1, dfrA17-aadA5, and aadA1 arrays. These findings illustrate the importance of WWTPs in spreading of resistance genes in the environment and the need for inclusion of at least monitoring efforts in the regular WWTP processes.

Show MeSH
Related in: MedlinePlus