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Qualitative and quantitative analysis of the major constituents in Chinese medical preparation Lianhua-Qingwen capsule by UPLC-DAD-QTOF-MS.

Jia W, Wang C, Wang Y, Pan G, Jiang M, Li Z, Zhu Y - ScientificWorldJournal (2015)

Bottom Line: Among them, twelve representative compounds were further quantified as chemical markers in quantitative analysis, including salidroside, chlorogenic acid, forsythoside E, cryptochlorogenic acid, amygdalin, sweroside, hyperin, rutin, forsythoside A, phillyrin, rhein, and glycyrrhizic acid.The UPLC-DAD method was evaluated with linearity, limit of detection (LOD), limit of quantification (LOQ), precision, stability, repeatability, and recovery tests.The results showed that the developed quantitative method was linear, sensitive, and precise for the quality control of LQC.

View Article: PubMed Central - PubMed

Affiliation: Tianjin Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China ; Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China ; Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology & Medicine, Tianjin 300457, China.

ABSTRACT
Lianhua-Qingwen capsule (LQC) is a commonly used Chinese medical preparation to treat viral influenza and especially played a very important role in the fight against severe acute respiratory syndrome (SARS) in 2002-2003 in China. In this paper, a rapid ultraperformance liquid chromatography coupled with diode-array detector and quadrupole time-of-flight mass spectrometry (UPLC-DAD-QTOF-MS) method was established for qualitative and quantitative analysis of the major constituents of LQC. A total of 61 compounds including flavonoids, phenylpropanoids, anthraquinones, triterpenoids, iridoids, and other types of compounds were unambiguously or tentatively identified by comparing the retention times and accurate mass measurement with reference compounds or literature data. Among them, twelve representative compounds were further quantified as chemical markers in quantitative analysis, including salidroside, chlorogenic acid, forsythoside E, cryptochlorogenic acid, amygdalin, sweroside, hyperin, rutin, forsythoside A, phillyrin, rhein, and glycyrrhizic acid. The UPLC-DAD method was evaluated with linearity, limit of detection (LOD), limit of quantification (LOQ), precision, stability, repeatability, and recovery tests. The results showed that the developed quantitative method was linear, sensitive, and precise for the quality control of LQC.

No MeSH data available.


Related in: MedlinePlus

UPLC-DAD chromatograms of standard solution of 210 nm (a), 225 nm (b), 254 nm (c), negative sample solution (d), and sample solution of 210 nm (e), 225 nm (f), and 254 nm (g).
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fig1: UPLC-DAD chromatograms of standard solution of 210 nm (a), 225 nm (b), 254 nm (c), negative sample solution (d), and sample solution of 210 nm (e), 225 nm (f), and 254 nm (g).

Mentions: Due to the existence of acidic constituents in sample solutions, formic acid was added into the mobile phase which could inhibit the ionization of these acidic ingredients to improve the peak shape. The mobile phase systems (methanol-formic acid aqueous solution and acetonitrile-formic acid aqueous solution) and column temperature (40°C and 50°C) were investigated, which showed that methanol-0.1% formic acid aqueous solution as mobile phase with column temperature at 50°C could obtain the best chromatographic peak shape. Because the maximum absorptions of 12 reference compounds were different, three detection wavelengths were finally selected in order to achieve the goal of high detection sensitivity and little interference. Forsythoside E (peak 7), cryptochlorogenic acid (peak 9), amygdalin (peak 33), and phillyrin (peak 39) had satisfactory sensitivity at 210 nm, salidroside (peak 6), chlorogenic acid (peak 8), and rhein (peak 54) at 225 nm, and sweroside (peak 13), hyperin (peak 26), rutin (peak 29), forsythoside A (peak 30), and glycyrrhizic acid (peak 57) at 254 nm. The chromatograms are presented in Figure 1.


Qualitative and quantitative analysis of the major constituents in Chinese medical preparation Lianhua-Qingwen capsule by UPLC-DAD-QTOF-MS.

Jia W, Wang C, Wang Y, Pan G, Jiang M, Li Z, Zhu Y - ScientificWorldJournal (2015)

UPLC-DAD chromatograms of standard solution of 210 nm (a), 225 nm (b), 254 nm (c), negative sample solution (d), and sample solution of 210 nm (e), 225 nm (f), and 254 nm (g).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4308632&req=5

fig1: UPLC-DAD chromatograms of standard solution of 210 nm (a), 225 nm (b), 254 nm (c), negative sample solution (d), and sample solution of 210 nm (e), 225 nm (f), and 254 nm (g).
Mentions: Due to the existence of acidic constituents in sample solutions, formic acid was added into the mobile phase which could inhibit the ionization of these acidic ingredients to improve the peak shape. The mobile phase systems (methanol-formic acid aqueous solution and acetonitrile-formic acid aqueous solution) and column temperature (40°C and 50°C) were investigated, which showed that methanol-0.1% formic acid aqueous solution as mobile phase with column temperature at 50°C could obtain the best chromatographic peak shape. Because the maximum absorptions of 12 reference compounds were different, three detection wavelengths were finally selected in order to achieve the goal of high detection sensitivity and little interference. Forsythoside E (peak 7), cryptochlorogenic acid (peak 9), amygdalin (peak 33), and phillyrin (peak 39) had satisfactory sensitivity at 210 nm, salidroside (peak 6), chlorogenic acid (peak 8), and rhein (peak 54) at 225 nm, and sweroside (peak 13), hyperin (peak 26), rutin (peak 29), forsythoside A (peak 30), and glycyrrhizic acid (peak 57) at 254 nm. The chromatograms are presented in Figure 1.

Bottom Line: Among them, twelve representative compounds were further quantified as chemical markers in quantitative analysis, including salidroside, chlorogenic acid, forsythoside E, cryptochlorogenic acid, amygdalin, sweroside, hyperin, rutin, forsythoside A, phillyrin, rhein, and glycyrrhizic acid.The UPLC-DAD method was evaluated with linearity, limit of detection (LOD), limit of quantification (LOQ), precision, stability, repeatability, and recovery tests.The results showed that the developed quantitative method was linear, sensitive, and precise for the quality control of LQC.

View Article: PubMed Central - PubMed

Affiliation: Tianjin Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China ; Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China ; Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology & Medicine, Tianjin 300457, China.

ABSTRACT
Lianhua-Qingwen capsule (LQC) is a commonly used Chinese medical preparation to treat viral influenza and especially played a very important role in the fight against severe acute respiratory syndrome (SARS) in 2002-2003 in China. In this paper, a rapid ultraperformance liquid chromatography coupled with diode-array detector and quadrupole time-of-flight mass spectrometry (UPLC-DAD-QTOF-MS) method was established for qualitative and quantitative analysis of the major constituents of LQC. A total of 61 compounds including flavonoids, phenylpropanoids, anthraquinones, triterpenoids, iridoids, and other types of compounds were unambiguously or tentatively identified by comparing the retention times and accurate mass measurement with reference compounds or literature data. Among them, twelve representative compounds were further quantified as chemical markers in quantitative analysis, including salidroside, chlorogenic acid, forsythoside E, cryptochlorogenic acid, amygdalin, sweroside, hyperin, rutin, forsythoside A, phillyrin, rhein, and glycyrrhizic acid. The UPLC-DAD method was evaluated with linearity, limit of detection (LOD), limit of quantification (LOQ), precision, stability, repeatability, and recovery tests. The results showed that the developed quantitative method was linear, sensitive, and precise for the quality control of LQC.

No MeSH data available.


Related in: MedlinePlus