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Immunolocalization of markers for bone formation during guided bone regeneration in osteopenic rats.

Tera Tde M, Nascimento RD, Prado RF, Santamaria MP, Jardini MA - J Appl Oral Sci (2014 Nov-Dec)

Bottom Line: Each of these groups was again divided into groups with either placement of an autogenous bone graft alone (BG) or an autogenous bone graft associated with an e-PTFE membrane (BGM).All groups (OVX+BG, OVX+BMG, SHAM+BG, and SHAM+BMG) showed greater bone formation, observed between 7 and 21 days, when BSP and ONC staining were more intense.However, the markers could be influenced by the presence of the e-PTFE membrane.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioscience and Oral Diagnosis, Instituto de Ciência e Tecnologia, Univ. Estadual Paulista, São José dos Campos, SP, Brazil.

ABSTRACT

Objective: The aim of this paper was to evaluate the repair of onlay autogenous bone grafts covered or not covered by an expanded polytetrafluoroethylene (e-PTFE) membrane using immunohistochemistry in rats with induced estrogen deficiency.

Material and methods: Eighty female rats were randomly divided into two groups: ovariectomized (OVX) and with a simulation of the surgical procedure (SHAM). Each of these groups was again divided into groups with either placement of an autogenous bone graft alone (BG) or an autogenous bone graft associated with an e-PTFE membrane (BGM). Animals were euthanized on days 0, 7, 21, 45, and 60. The specimens were subjected to immunohistochemistry for bone sialoprotein (BSP), osteonectin (ONC), and osteocalcin (OCC).

Results: All groups (OVX+BG, OVX+BMG, SHAM+BG, and SHAM+BMG) showed greater bone formation, observed between 7 and 21 days, when BSP and ONC staining were more intense. At the 45-day, the bone graft showed direct bonding to the recipient bed in all specimens. The ONC and OCC showed more expressed in granulation tissue, in the membrane groups, independently of estrogen deficiency.

Conclusions: The expression of bone forming markers was not negatively influenced by estrogen deficiency. However, the markers could be influenced by the presence of the e-PTFE membrane.

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Related in: MedlinePlus

Surgical procedure: a) the calvarium was used as the donor area to graft removed; b) angle of the mandible was the recipient area; c) recipient bed; d) perforation was made at the angle of the mandible, which enabled the stable attachment of the bone block; e) bone block in position; f) e-PTFE membrane was adapted, covering the bone graft in the OVX+BGM and SHAM+BGM
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f01: Surgical procedure: a) the calvarium was used as the donor area to graft removed; b) angle of the mandible was the recipient area; c) recipient bed; d) perforation was made at the angle of the mandible, which enabled the stable attachment of the bone block; e) bone block in position; f) e-PTFE membrane was adapted, covering the bone graft in the OVX+BGM and SHAM+BGM

Mentions: After 30 days, a new surgical procedure for the placement of autogenous bone grafts was performed as described by Jardini, et al.13 (2005). The calvarium was used as the donor area, and the angle of the mandible was the recipient area. The graft was initially removed using a trephine drill with an external diameter of 4.1 mm (Neodent, São Paulo, SP, Brazil) and then punctured in the center using a ½ carbide bur (KG Sorensen, Cotia, SP, Brazil) at low rotation and cooled with saline. Three perforations were made at the angle of the mandible using the ½ carbide bur under cooling conditions, which enabled the stable attachment of the bone block to the recipient bed using a 5.0 green braided polyester suture (Ethicon, Johnson & Johnson, São José dos Campos, SP, Brazil). Afterward, the e-PTFE membrane was adapted, covering the bone graft in the OVX+BGM and SHAM+BGM. These procedures allowed close contact between the graft and the surface of the mandibular bone (Figure 1).


Immunolocalization of markers for bone formation during guided bone regeneration in osteopenic rats.

Tera Tde M, Nascimento RD, Prado RF, Santamaria MP, Jardini MA - J Appl Oral Sci (2014 Nov-Dec)

Surgical procedure: a) the calvarium was used as the donor area to graft removed; b) angle of the mandible was the recipient area; c) recipient bed; d) perforation was made at the angle of the mandible, which enabled the stable attachment of the bone block; e) bone block in position; f) e-PTFE membrane was adapted, covering the bone graft in the OVX+BGM and SHAM+BGM
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307769&req=5

f01: Surgical procedure: a) the calvarium was used as the donor area to graft removed; b) angle of the mandible was the recipient area; c) recipient bed; d) perforation was made at the angle of the mandible, which enabled the stable attachment of the bone block; e) bone block in position; f) e-PTFE membrane was adapted, covering the bone graft in the OVX+BGM and SHAM+BGM
Mentions: After 30 days, a new surgical procedure for the placement of autogenous bone grafts was performed as described by Jardini, et al.13 (2005). The calvarium was used as the donor area, and the angle of the mandible was the recipient area. The graft was initially removed using a trephine drill with an external diameter of 4.1 mm (Neodent, São Paulo, SP, Brazil) and then punctured in the center using a ½ carbide bur (KG Sorensen, Cotia, SP, Brazil) at low rotation and cooled with saline. Three perforations were made at the angle of the mandible using the ½ carbide bur under cooling conditions, which enabled the stable attachment of the bone block to the recipient bed using a 5.0 green braided polyester suture (Ethicon, Johnson & Johnson, São José dos Campos, SP, Brazil). Afterward, the e-PTFE membrane was adapted, covering the bone graft in the OVX+BGM and SHAM+BGM. These procedures allowed close contact between the graft and the surface of the mandibular bone (Figure 1).

Bottom Line: Each of these groups was again divided into groups with either placement of an autogenous bone graft alone (BG) or an autogenous bone graft associated with an e-PTFE membrane (BGM).All groups (OVX+BG, OVX+BMG, SHAM+BG, and SHAM+BMG) showed greater bone formation, observed between 7 and 21 days, when BSP and ONC staining were more intense.However, the markers could be influenced by the presence of the e-PTFE membrane.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioscience and Oral Diagnosis, Instituto de Ciência e Tecnologia, Univ. Estadual Paulista, São José dos Campos, SP, Brazil.

ABSTRACT

Objective: The aim of this paper was to evaluate the repair of onlay autogenous bone grafts covered or not covered by an expanded polytetrafluoroethylene (e-PTFE) membrane using immunohistochemistry in rats with induced estrogen deficiency.

Material and methods: Eighty female rats were randomly divided into two groups: ovariectomized (OVX) and with a simulation of the surgical procedure (SHAM). Each of these groups was again divided into groups with either placement of an autogenous bone graft alone (BG) or an autogenous bone graft associated with an e-PTFE membrane (BGM). Animals were euthanized on days 0, 7, 21, 45, and 60. The specimens were subjected to immunohistochemistry for bone sialoprotein (BSP), osteonectin (ONC), and osteocalcin (OCC).

Results: All groups (OVX+BG, OVX+BMG, SHAM+BG, and SHAM+BMG) showed greater bone formation, observed between 7 and 21 days, when BSP and ONC staining were more intense. At the 45-day, the bone graft showed direct bonding to the recipient bed in all specimens. The ONC and OCC showed more expressed in granulation tissue, in the membrane groups, independently of estrogen deficiency.

Conclusions: The expression of bone forming markers was not negatively influenced by estrogen deficiency. However, the markers could be influenced by the presence of the e-PTFE membrane.

Show MeSH
Related in: MedlinePlus