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Up-regulation of miR-9 target CBX7 to regulate invasion ability of bladder transitional cell carcinoma.

Xie D, Shang C, Zhang H, Guo Y, Tong X - Med. Sci. Monit. (2015)

Bottom Line: The expression of miR-9 increased significantly in human TCC specimens compared to that in NBTC specimens.The pcDNA-CBX7 transfection could up-regulate the CBX7 protein expression, and the invasion ability of T24 cells with CBX7 over-expression decreased significantly.Aberrantly expressed miR-9 contributes to T24 cells invasion, partly through directly down-regulating CBX7 protein expression in TCC.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, College of Basic Medicine, China Medical University, Shenyang, Liaoning, China (mainland).

ABSTRACT

Background: Bladder urothelial carcinoma is the most common genitourinary system cancer in China. The objective of this study was to investigate whether the miR-9 can regulate the invasion ability of human bladder transitional cell carcinoma cells by down-regulation of CBX7.

Material/methods: The expression of miR-9 was detected by quantitative real-time PCR in bladder transitional cell carcinomas (TCC) and normal bladder transitional cell (NBTC) samples. Bioinformatics software was used to predict some potential target genes of miR-9. T24 cells were transfected with pre-miR-9, and the CBX7 protein expression was detected by Western blot. Luciferase activities assay was selected to verify that CBX7 was a direct and specific gene of miR-9. T24 cells were transfected with pcDNA-CBX7, and the expression of CBX7 gene was detected. Then, the transwell assay was used to detect the invasion ability of T24 cells with CBX7 over-expression.

Results: The expression of miR-9 increased significantly in human TCC specimens compared to that in NBTC specimens. TargetScan and PicTar software programs predicted CBX7 gene was a target gene of miR-9. The pre-miR-9 could up-regulate the miR-9 expression and down-regulate CBX7 protein expression. The luciferase activities assay verified that CBX7 gene was a direct and specific target gene of miR-9. The pcDNA-CBX7 transfection could up-regulate the CBX7 protein expression, and the invasion ability of T24 cells with CBX7 over-expression decreased significantly.

Conclusions: Aberrantly expressed miR-9 contributes to T24 cells invasion, partly through directly down-regulating CBX7 protein expression in TCC. This miRNA signature offers a new potential therapeutic target for TCC.

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Related in: MedlinePlus

qRT-PCR analysis for the expression of miR-9 in NTBC and TCC samples. (A) Compared to NTBC, the expression of miR-9 was higher in TCC, * P<0.01 vs. NTBC. (B) Compared to superficial TCC, the expression of miR-9 was higher in invasive TCC, * P<0.01 vs. superficial TCC.
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f1-medscimonit-21-225: qRT-PCR analysis for the expression of miR-9 in NTBC and TCC samples. (A) Compared to NTBC, the expression of miR-9 was higher in TCC, * P<0.01 vs. NTBC. (B) Compared to superficial TCC, the expression of miR-9 was higher in invasive TCC, * P<0.01 vs. superficial TCC.

Mentions: To search for the possible effect of miR-9 in TCC, the expression level of miR-9 was first detected in TCC and NBTC by SYBR-Green quantitative real-time PCR. The experiment results showed that, in contrast with NBTC tissues, the miR-9 expression in TCC tissues were significantly (p<0.01) up-regulated (Figure 1A). Those results indicate that miR-9 might be involved in TCC development. Further, the expression of miR-9 in invasive TCC tissue was significantly higher than that of superficial TCC (p<0.01) (Figure 1B).


Up-regulation of miR-9 target CBX7 to regulate invasion ability of bladder transitional cell carcinoma.

Xie D, Shang C, Zhang H, Guo Y, Tong X - Med. Sci. Monit. (2015)

qRT-PCR analysis for the expression of miR-9 in NTBC and TCC samples. (A) Compared to NTBC, the expression of miR-9 was higher in TCC, * P<0.01 vs. NTBC. (B) Compared to superficial TCC, the expression of miR-9 was higher in invasive TCC, * P<0.01 vs. superficial TCC.
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4307688&req=5

f1-medscimonit-21-225: qRT-PCR analysis for the expression of miR-9 in NTBC and TCC samples. (A) Compared to NTBC, the expression of miR-9 was higher in TCC, * P<0.01 vs. NTBC. (B) Compared to superficial TCC, the expression of miR-9 was higher in invasive TCC, * P<0.01 vs. superficial TCC.
Mentions: To search for the possible effect of miR-9 in TCC, the expression level of miR-9 was first detected in TCC and NBTC by SYBR-Green quantitative real-time PCR. The experiment results showed that, in contrast with NBTC tissues, the miR-9 expression in TCC tissues were significantly (p<0.01) up-regulated (Figure 1A). Those results indicate that miR-9 might be involved in TCC development. Further, the expression of miR-9 in invasive TCC tissue was significantly higher than that of superficial TCC (p<0.01) (Figure 1B).

Bottom Line: The expression of miR-9 increased significantly in human TCC specimens compared to that in NBTC specimens.The pcDNA-CBX7 transfection could up-regulate the CBX7 protein expression, and the invasion ability of T24 cells with CBX7 over-expression decreased significantly.Aberrantly expressed miR-9 contributes to T24 cells invasion, partly through directly down-regulating CBX7 protein expression in TCC.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, College of Basic Medicine, China Medical University, Shenyang, Liaoning, China (mainland).

ABSTRACT

Background: Bladder urothelial carcinoma is the most common genitourinary system cancer in China. The objective of this study was to investigate whether the miR-9 can regulate the invasion ability of human bladder transitional cell carcinoma cells by down-regulation of CBX7.

Material/methods: The expression of miR-9 was detected by quantitative real-time PCR in bladder transitional cell carcinomas (TCC) and normal bladder transitional cell (NBTC) samples. Bioinformatics software was used to predict some potential target genes of miR-9. T24 cells were transfected with pre-miR-9, and the CBX7 protein expression was detected by Western blot. Luciferase activities assay was selected to verify that CBX7 was a direct and specific gene of miR-9. T24 cells were transfected with pcDNA-CBX7, and the expression of CBX7 gene was detected. Then, the transwell assay was used to detect the invasion ability of T24 cells with CBX7 over-expression.

Results: The expression of miR-9 increased significantly in human TCC specimens compared to that in NBTC specimens. TargetScan and PicTar software programs predicted CBX7 gene was a target gene of miR-9. The pre-miR-9 could up-regulate the miR-9 expression and down-regulate CBX7 protein expression. The luciferase activities assay verified that CBX7 gene was a direct and specific target gene of miR-9. The pcDNA-CBX7 transfection could up-regulate the CBX7 protein expression, and the invasion ability of T24 cells with CBX7 over-expression decreased significantly.

Conclusions: Aberrantly expressed miR-9 contributes to T24 cells invasion, partly through directly down-regulating CBX7 protein expression in TCC. This miRNA signature offers a new potential therapeutic target for TCC.

Show MeSH
Related in: MedlinePlus