Limits...
Validation of Urinary Glycosaminoglycans in Iranian patients with Mucopolysaccharidase type I: The effect of urine sedimentation characteristics.

Abdi M, Hakhamaneshi MS, Alaei MR, Azadi NA, Vakili R, Zamanfar D, Taghikhani M, Khatami S - Iran J Child Neurol (2014)

Bottom Line: Urine total glycosaminoglycans were significantly higher in male subjects than in female subjects.Glycosaminoglycan concentration was markedly decreased in specimens with elevated white blood cell and epithelial cells count.Urine glycosaminoglycans concentration significantly differs in our studied population.

View Article: PubMed Central - PubMed

Affiliation: .Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

ABSTRACT

Objective: The first line-screening test for mucopolysaccharidosis is based on measurement of urinary glycosaminoglycans. The most reliable test for measurement of urine glycosaminoglycans is the 1,9-dimethyleneblue colorimetric assay. Biological markers are affected by ethnical factors, for this reason, the World Health Organization recommends that the diagnostic test characteristics should be used to determine results for different populations. This study determines the diagnostic value of 1,9-dimethyleneblue tests for diagnosis of mucopolysaccharidosis type I patients in Iran.

Materials & methods: In addition to routine urine analysis, the qualitative and quantitative measurements of urine glucosaminoglycans were performed with the Berry spot test and 1,9-dimethyleneblue assay. Diagnostic values of the tests were determined using the ROC curve.

Results: Urine total glycosaminoglycans were significantly higher in male subjects than in female subjects. Glycosaminoglycan concentration was markedly decreased in specimens with elevated white blood cell and epithelial cells count. Using a cut-off level of 10.37 mg/g creatinine, sensitivity, and specificity were 100% and 97.22%, respectively, for a 1,9-dimethyleneblue colorimetric assay.

Conclusion: Urine glycosaminoglycans concentration significantly differs in our studied population. In addition to determine diagnostic validity of the 1,9-dimethyleneblue test, our results demonstrate the usefulness of measuring glycosaminoglycans for early screening of mucopolysaccharidosis type I Iran.

No MeSH data available.


Related in: MedlinePlus

Concentrations of urinary GAGs according to gender
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4307367&req=5

Figure 1: Concentrations of urinary GAGs according to gender

Mentions: In addition, we evaluated the change of GAGs concentration in male and female subjects. Based on the DMB method, there was a significant differences between the urine GAGs in male and female subjects (Figure 1). Male subjects showed significantly (p<0.05) higher urine GAGs level than the female group did (7.21± 3.68 and 4.4 ± 3.44 mg/g creatinine, respectively).


Validation of Urinary Glycosaminoglycans in Iranian patients with Mucopolysaccharidase type I: The effect of urine sedimentation characteristics.

Abdi M, Hakhamaneshi MS, Alaei MR, Azadi NA, Vakili R, Zamanfar D, Taghikhani M, Khatami S - Iran J Child Neurol (2014)

Concentrations of urinary GAGs according to gender
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307367&req=5

Figure 1: Concentrations of urinary GAGs according to gender
Mentions: In addition, we evaluated the change of GAGs concentration in male and female subjects. Based on the DMB method, there was a significant differences between the urine GAGs in male and female subjects (Figure 1). Male subjects showed significantly (p<0.05) higher urine GAGs level than the female group did (7.21± 3.68 and 4.4 ± 3.44 mg/g creatinine, respectively).

Bottom Line: Urine total glycosaminoglycans were significantly higher in male subjects than in female subjects.Glycosaminoglycan concentration was markedly decreased in specimens with elevated white blood cell and epithelial cells count.Urine glycosaminoglycans concentration significantly differs in our studied population.

View Article: PubMed Central - PubMed

Affiliation: .Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

ABSTRACT

Objective: The first line-screening test for mucopolysaccharidosis is based on measurement of urinary glycosaminoglycans. The most reliable test for measurement of urine glycosaminoglycans is the 1,9-dimethyleneblue colorimetric assay. Biological markers are affected by ethnical factors, for this reason, the World Health Organization recommends that the diagnostic test characteristics should be used to determine results for different populations. This study determines the diagnostic value of 1,9-dimethyleneblue tests for diagnosis of mucopolysaccharidosis type I patients in Iran.

Materials & methods: In addition to routine urine analysis, the qualitative and quantitative measurements of urine glucosaminoglycans were performed with the Berry spot test and 1,9-dimethyleneblue assay. Diagnostic values of the tests were determined using the ROC curve.

Results: Urine total glycosaminoglycans were significantly higher in male subjects than in female subjects. Glycosaminoglycan concentration was markedly decreased in specimens with elevated white blood cell and epithelial cells count. Using a cut-off level of 10.37 mg/g creatinine, sensitivity, and specificity were 100% and 97.22%, respectively, for a 1,9-dimethyleneblue colorimetric assay.

Conclusion: Urine glycosaminoglycans concentration significantly differs in our studied population. In addition to determine diagnostic validity of the 1,9-dimethyleneblue test, our results demonstrate the usefulness of measuring glycosaminoglycans for early screening of mucopolysaccharidosis type I Iran.

No MeSH data available.


Related in: MedlinePlus