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Molecular characterization of a new wheat-Thinopyrum intermedium translocation line with resistance to powdery mildew and stripe rust.

Zhan H, Zhang X, Li G, Pan Z, Hu J, Li X, Qiao L, Jia J, Guo H, Chang Z, Yang Z - Int J Mol Sci (2015)

Bottom Line: The molecular markers analysis further confirmed that the introduced Th. intermedium chromatin in CH13-21 belonged to the long arm of homoeologous group 6 chromosome.Therefore, CH13-21 was a new T6BS.6Ai#1L compensating Robertsonian translocation line.It concludes that CH13-21 is a new genetic resource for wheat breeding programs providing novel variation for disease resistances.

View Article: PubMed Central - PubMed

Affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. zhan030006@126.com.

ABSTRACT
A new wheat-Thinopyrum translocation line CH13-21 was selected from the progenies derived from a cross between wheat-Th. intermedium partial amphiploid TAI7047 and wheat line Mianyang11. CH13-21 was characterized by using genomic in situ hybridization (GISH), multicolor-GISH (mc-GISH), multicolor-fluorescence in situ hybridization (mc-FISH) and chromosome-specific molecular markers. When inoculated with stripe rust and powdery mildew isolates, CH13-21 displayed novel resistance to powdery mildew and stripe rust which inherited from its Thinopyrum parent. The chromosomal counting analyses indicated that CH13-21 has 42 chromosomes, with normal bivalent pairing at metaphase I of meiosis. GISH probed by Th. intermedium genomic DNA showed that CH13-21 contained a pair of wheat-Th. intermedium translocated chromosomes. Sequential mc-FISH analyses probed by pSc119.2 and pAs1 clearly revealed that chromosome arm 6BS of CH13-21 was replaced by Thinopyrum chromatin in the translocation chromosome. The molecular markers analysis further confirmed that the introduced Th. intermedium chromatin in CH13-21 belonged to the long arm of homoeologous group 6 chromosome. Therefore, CH13-21 was a new T6BS.6Ai#1L compensating Robertsonian translocation line. It concludes that CH13-21 is a new genetic resource for wheat breeding programs providing novel variation for disease resistances.

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PCR amplification using primers TNAC1726 (A); TNAC1702 (B) and TNAC1752 (C). The arrows indicates the Th. intermedium specific bands.
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ijms-16-02162-f003: PCR amplification using primers TNAC1726 (A); TNAC1702 (B) and TNAC1752 (C). The arrows indicates the Th. intermedium specific bands.

Mentions: The PCR-based landmark unique gene (PLUG) primers can amplify the diagnostic fragments in wheat homoeologous groups of individual chromosomes, and further determine the presence and absent of the specific chromosomal segments [15]. A total of 155 PLUG markers distributed over wheat homologous groups 1-7 were used to amplify the polymorphic bands among CH13-21, MY11, TAI7047 and Th. intermedium. We found that 10 PLUG markers from wheat homoeologous group 6 generated Th. intermedium specific bands in CH13-21, compared to those of its parents MY11 and TAI7047 (Table 2). As shown in Figure 3, the PLUG primers TNAC1752 and TNAC1702 amplified specific fragments from chromosomes 6A, 6B, and 6D of wheat revealed by Chinese Spring (CS) i-tetrasomic lines. The amplification demonstrated that the 6BL specific fragment was absent in CH13-21, which is consistent with the result of chromatin 6BL being absent in line CH13-21 revealed by FISH (Figure 2). The amplification also indicated that Th. intermedium-specific bands appeared in line CH13-21, suggesting that the Th. intermedium chromatin in CH13-21 attributed to homoeologous group 6 (Table 2, Figure 3). Therefore, we concluded that the CH13-21 was a wheat-Th. intermedium T6BS.6Ai#1L translocation line.


Molecular characterization of a new wheat-Thinopyrum intermedium translocation line with resistance to powdery mildew and stripe rust.

Zhan H, Zhang X, Li G, Pan Z, Hu J, Li X, Qiao L, Jia J, Guo H, Chang Z, Yang Z - Int J Mol Sci (2015)

PCR amplification using primers TNAC1726 (A); TNAC1702 (B) and TNAC1752 (C). The arrows indicates the Th. intermedium specific bands.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307355&req=5

ijms-16-02162-f003: PCR amplification using primers TNAC1726 (A); TNAC1702 (B) and TNAC1752 (C). The arrows indicates the Th. intermedium specific bands.
Mentions: The PCR-based landmark unique gene (PLUG) primers can amplify the diagnostic fragments in wheat homoeologous groups of individual chromosomes, and further determine the presence and absent of the specific chromosomal segments [15]. A total of 155 PLUG markers distributed over wheat homologous groups 1-7 were used to amplify the polymorphic bands among CH13-21, MY11, TAI7047 and Th. intermedium. We found that 10 PLUG markers from wheat homoeologous group 6 generated Th. intermedium specific bands in CH13-21, compared to those of its parents MY11 and TAI7047 (Table 2). As shown in Figure 3, the PLUG primers TNAC1752 and TNAC1702 amplified specific fragments from chromosomes 6A, 6B, and 6D of wheat revealed by Chinese Spring (CS) i-tetrasomic lines. The amplification demonstrated that the 6BL specific fragment was absent in CH13-21, which is consistent with the result of chromatin 6BL being absent in line CH13-21 revealed by FISH (Figure 2). The amplification also indicated that Th. intermedium-specific bands appeared in line CH13-21, suggesting that the Th. intermedium chromatin in CH13-21 attributed to homoeologous group 6 (Table 2, Figure 3). Therefore, we concluded that the CH13-21 was a wheat-Th. intermedium T6BS.6Ai#1L translocation line.

Bottom Line: The molecular markers analysis further confirmed that the introduced Th. intermedium chromatin in CH13-21 belonged to the long arm of homoeologous group 6 chromosome.Therefore, CH13-21 was a new T6BS.6Ai#1L compensating Robertsonian translocation line.It concludes that CH13-21 is a new genetic resource for wheat breeding programs providing novel variation for disease resistances.

View Article: PubMed Central - PubMed

Affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. zhan030006@126.com.

ABSTRACT
A new wheat-Thinopyrum translocation line CH13-21 was selected from the progenies derived from a cross between wheat-Th. intermedium partial amphiploid TAI7047 and wheat line Mianyang11. CH13-21 was characterized by using genomic in situ hybridization (GISH), multicolor-GISH (mc-GISH), multicolor-fluorescence in situ hybridization (mc-FISH) and chromosome-specific molecular markers. When inoculated with stripe rust and powdery mildew isolates, CH13-21 displayed novel resistance to powdery mildew and stripe rust which inherited from its Thinopyrum parent. The chromosomal counting analyses indicated that CH13-21 has 42 chromosomes, with normal bivalent pairing at metaphase I of meiosis. GISH probed by Th. intermedium genomic DNA showed that CH13-21 contained a pair of wheat-Th. intermedium translocated chromosomes. Sequential mc-FISH analyses probed by pSc119.2 and pAs1 clearly revealed that chromosome arm 6BS of CH13-21 was replaced by Thinopyrum chromatin in the translocation chromosome. The molecular markers analysis further confirmed that the introduced Th. intermedium chromatin in CH13-21 belonged to the long arm of homoeologous group 6 chromosome. Therefore, CH13-21 was a new T6BS.6Ai#1L compensating Robertsonian translocation line. It concludes that CH13-21 is a new genetic resource for wheat breeding programs providing novel variation for disease resistances.

Show MeSH
Related in: MedlinePlus