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Running exercise alleviates pain and promotes cell proliferation in a rat model of intervertebral disc degeneration.

Luan S, Wan Q, Luo H, Li X, Ke S, Lin C, Wu Y, Wu S, Ma C - Int J Mol Sci (2015)

Bottom Line: Chronic low back pain accompanied by intervertebral disk degeneration is a common musculoskeletal disorder.The results of the present study showed that mechanical withdrawal thresholds of bilateral hindpaw were significantly decreased beginning on day three after intradiscal complete Freund's adjuvant (CFA) injection and daily running exercise remarkably reduced allodynia in the CFA exercise group beginning at day 28 compared to the spontaneous recovery group (controls).Taken together, these results suggest that running exercise might alleviate the mechanical allodynia induced by intradiscal CFA injection via disc repair and cell proliferation, which provides new evidence for future clinical use.

View Article: PubMed Central - PubMed

Affiliation: Department of Rehabilitation Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China. luanshuo126@163.com.

ABSTRACT
Chronic low back pain accompanied by intervertebral disk degeneration is a common musculoskeletal disorder. Physical exercise, which is clinically recommended by international guidelines, has proven to be effective for degenerative disc disease (DDD) patients. However, the mechanism underlying the analgesic effects of physical exercise on DDD remains largely unclear. The results of the present study showed that mechanical withdrawal thresholds of bilateral hindpaw were significantly decreased beginning on day three after intradiscal complete Freund's adjuvant (CFA) injection and daily running exercise remarkably reduced allodynia in the CFA exercise group beginning at day 28 compared to the spontaneous recovery group (controls). The hindpaw withdrawal thresholds of the exercise group returned nearly to baseline at the end of experiment, but severe pain persisted in the control group. Histological examinations performed on day 70 revealed that running exercise restored the degenerative discs and increased the cell densities of the annulus fibrosus (AF) and nucleus pulposus (NP). Furthermore, immunofluorescence labeling revealed significantly higher numbers of 5-bromo-2-deoxyuridine (BrdU)-positive cells in the exercise group on days 28, 42, 56 and 70, which indicated more rapid proliferation compared to the control at the corresponding time points. Taken together, these results suggest that running exercise might alleviate the mechanical allodynia induced by intradiscal CFA injection via disc repair and cell proliferation, which provides new evidence for future clinical use.

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The correlations between the mechanical withdrawal thresholds and the cell counts of CFA exercise group. (A) The mechanical withdrawal thresholds were closely related to the increased cell numbers of nucleus pulposus (NP) (r = 0.98, p ˂ 0.01); (B) The tight correlation was also observed in annulus fibrosus (AF) (r = 0.96, p ˂ 0.01). The correlations between pain relief and cell counts of NP and AF were determined by Spearman rank correlation. n = 8 in each CFA exercise group.
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ijms-16-02130-f004: The correlations between the mechanical withdrawal thresholds and the cell counts of CFA exercise group. (A) The mechanical withdrawal thresholds were closely related to the increased cell numbers of nucleus pulposus (NP) (r = 0.98, p ˂ 0.01); (B) The tight correlation was also observed in annulus fibrosus (AF) (r = 0.96, p ˂ 0.01). The correlations between pain relief and cell counts of NP and AF were determined by Spearman rank correlation. n = 8 in each CFA exercise group.

Mentions: To explore the relationship between the pain relief and cell proliferation in the CFA exercise group, we further performed the correlation analysis based on the results of the mechanical withdrawal thresholds and cell counts within NP and AF. The results showed that the withdrawal thresholds of CFA model rats increased positively with the increased cell numbers of both NP (r = 0.98, p ˂ 0.01) and AF (r = 0.96, p ˂ 0.01), which indicated that running exercise might attenuate the CFA-induced mechanical allodynia by increasing cell densities within discs (Figure 4).


Running exercise alleviates pain and promotes cell proliferation in a rat model of intervertebral disc degeneration.

Luan S, Wan Q, Luo H, Li X, Ke S, Lin C, Wu Y, Wu S, Ma C - Int J Mol Sci (2015)

The correlations between the mechanical withdrawal thresholds and the cell counts of CFA exercise group. (A) The mechanical withdrawal thresholds were closely related to the increased cell numbers of nucleus pulposus (NP) (r = 0.98, p ˂ 0.01); (B) The tight correlation was also observed in annulus fibrosus (AF) (r = 0.96, p ˂ 0.01). The correlations between pain relief and cell counts of NP and AF were determined by Spearman rank correlation. n = 8 in each CFA exercise group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307353&req=5

ijms-16-02130-f004: The correlations between the mechanical withdrawal thresholds and the cell counts of CFA exercise group. (A) The mechanical withdrawal thresholds were closely related to the increased cell numbers of nucleus pulposus (NP) (r = 0.98, p ˂ 0.01); (B) The tight correlation was also observed in annulus fibrosus (AF) (r = 0.96, p ˂ 0.01). The correlations between pain relief and cell counts of NP and AF were determined by Spearman rank correlation. n = 8 in each CFA exercise group.
Mentions: To explore the relationship between the pain relief and cell proliferation in the CFA exercise group, we further performed the correlation analysis based on the results of the mechanical withdrawal thresholds and cell counts within NP and AF. The results showed that the withdrawal thresholds of CFA model rats increased positively with the increased cell numbers of both NP (r = 0.98, p ˂ 0.01) and AF (r = 0.96, p ˂ 0.01), which indicated that running exercise might attenuate the CFA-induced mechanical allodynia by increasing cell densities within discs (Figure 4).

Bottom Line: Chronic low back pain accompanied by intervertebral disk degeneration is a common musculoskeletal disorder.The results of the present study showed that mechanical withdrawal thresholds of bilateral hindpaw were significantly decreased beginning on day three after intradiscal complete Freund's adjuvant (CFA) injection and daily running exercise remarkably reduced allodynia in the CFA exercise group beginning at day 28 compared to the spontaneous recovery group (controls).Taken together, these results suggest that running exercise might alleviate the mechanical allodynia induced by intradiscal CFA injection via disc repair and cell proliferation, which provides new evidence for future clinical use.

View Article: PubMed Central - PubMed

Affiliation: Department of Rehabilitation Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China. luanshuo126@163.com.

ABSTRACT
Chronic low back pain accompanied by intervertebral disk degeneration is a common musculoskeletal disorder. Physical exercise, which is clinically recommended by international guidelines, has proven to be effective for degenerative disc disease (DDD) patients. However, the mechanism underlying the analgesic effects of physical exercise on DDD remains largely unclear. The results of the present study showed that mechanical withdrawal thresholds of bilateral hindpaw were significantly decreased beginning on day three after intradiscal complete Freund's adjuvant (CFA) injection and daily running exercise remarkably reduced allodynia in the CFA exercise group beginning at day 28 compared to the spontaneous recovery group (controls). The hindpaw withdrawal thresholds of the exercise group returned nearly to baseline at the end of experiment, but severe pain persisted in the control group. Histological examinations performed on day 70 revealed that running exercise restored the degenerative discs and increased the cell densities of the annulus fibrosus (AF) and nucleus pulposus (NP). Furthermore, immunofluorescence labeling revealed significantly higher numbers of 5-bromo-2-deoxyuridine (BrdU)-positive cells in the exercise group on days 28, 42, 56 and 70, which indicated more rapid proliferation compared to the control at the corresponding time points. Taken together, these results suggest that running exercise might alleviate the mechanical allodynia induced by intradiscal CFA injection via disc repair and cell proliferation, which provides new evidence for future clinical use.

Show MeSH
Related in: MedlinePlus