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Prevention of osteoporosis by oral administration of phytate-removed and deamidated soybean β-conglycinin.

Akao M, Abe R, Sato N, Hasegawa-Tanigome A, Kumagai H, Kumagai H - Int J Mol Sci (2015)

Bottom Line: The apparent calcium absorption rate decreased following ovariectomy and was not replenished by oral administration of phytate-removed soybean β-conglycinin (PrS) or casein.Markers of bone resorption, such as serum parathyroid hormone (PTH) and urinary deoxypyridinoline (DPD), increased, and the bone mineral density and breaking stress decreased following ovariectomy.However, PrDS supplementation suppressed the changes caused by the decrease in calcium absorption from the small intestine.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry and Life Science, Nihon University, 1866 Kameino, Fujisawa-shi, Kanagawa 252-0880, Japan. makao@brs.nihon-u.ac.jp.

ABSTRACT
Phytate-removed and deamidated soybean β-conglycinin (PrDS) prepared by ion-exchange resins was supplemented to be 4% in the diet administered to ovariectomized rats to investigate its preventive effect on osteoporosis. The apparent calcium absorption rate decreased following ovariectomy and was not replenished by oral administration of phytate-removed soybean β-conglycinin (PrS) or casein. On the other hand, administration of PrDS restored the calcium absorption rate to the same level as the sham group. Markers of bone resorption, such as serum parathyroid hormone (PTH) and urinary deoxypyridinoline (DPD), increased, and the bone mineral density and breaking stress decreased following ovariectomy. However, PrDS supplementation suppressed the changes caused by the decrease in calcium absorption from the small intestine. Therefore, PrDS supplementation shows promise for the prevention of postmenopausal osteoporosis.

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Related in: MedlinePlus

The urinary deoxypyridinoline (DPD) level in sham-treated and ovariectomized rats fed each experimental diet for eight weeks. Urinary DPD was standardized with urinary creatinine. Sham, non-ovariectomized and 20% egg albumin intake; control, ovariectomized and 20% egg albumin intake; PrS, ovariectomized and 4% PrS + 16% egg albumin intake; PrDS, ovariectomized and 4% PrDS + 16% egg albumin intake; casein, ovariectomized and 4% casein + 16% egg albumin intake. Values represent the means for six rats with the SE. Values indicated by different letters are significantly different at p < 0.05.
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ijms-16-02117-f003: The urinary deoxypyridinoline (DPD) level in sham-treated and ovariectomized rats fed each experimental diet for eight weeks. Urinary DPD was standardized with urinary creatinine. Sham, non-ovariectomized and 20% egg albumin intake; control, ovariectomized and 20% egg albumin intake; PrS, ovariectomized and 4% PrS + 16% egg albumin intake; PrDS, ovariectomized and 4% PrDS + 16% egg albumin intake; casein, ovariectomized and 4% casein + 16% egg albumin intake. Values represent the means for six rats with the SE. Values indicated by different letters are significantly different at p < 0.05.

Mentions: The level of deoxypyridinoline (DPD) is shown in Figure 3 (sham group: 83.31 ± 5.24 nmol/mmol creatinine; control: 142.34 ± 26.7 nmol/mmol creatinine; PrS: 140.82 ± 12.0 nmol/mmol creatinine; PrDS: 91.2 ± 4.04 nmol/mmol creatinine; casein: 118.05 ± 9.64 nmol/mmol creatinine). Type 1 collagen accounts for approximately 90% of the organic matrix of bone and is cross-linked by pyridinoline and DPD, which are released and excreted in the urine during bone resorption and collagen breakdown. Therefore, the presence of DPD in the urine is considered to be a marker of bone resorption [26]. The control, PrS and casein groups showed significantly higher DPD levels than the sham group. However, the DPD level of the PrDS group was almost identical to that of the sham group.


Prevention of osteoporosis by oral administration of phytate-removed and deamidated soybean β-conglycinin.

Akao M, Abe R, Sato N, Hasegawa-Tanigome A, Kumagai H, Kumagai H - Int J Mol Sci (2015)

The urinary deoxypyridinoline (DPD) level in sham-treated and ovariectomized rats fed each experimental diet for eight weeks. Urinary DPD was standardized with urinary creatinine. Sham, non-ovariectomized and 20% egg albumin intake; control, ovariectomized and 20% egg albumin intake; PrS, ovariectomized and 4% PrS + 16% egg albumin intake; PrDS, ovariectomized and 4% PrDS + 16% egg albumin intake; casein, ovariectomized and 4% casein + 16% egg albumin intake. Values represent the means for six rats with the SE. Values indicated by different letters are significantly different at p < 0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307352&req=5

ijms-16-02117-f003: The urinary deoxypyridinoline (DPD) level in sham-treated and ovariectomized rats fed each experimental diet for eight weeks. Urinary DPD was standardized with urinary creatinine. Sham, non-ovariectomized and 20% egg albumin intake; control, ovariectomized and 20% egg albumin intake; PrS, ovariectomized and 4% PrS + 16% egg albumin intake; PrDS, ovariectomized and 4% PrDS + 16% egg albumin intake; casein, ovariectomized and 4% casein + 16% egg albumin intake. Values represent the means for six rats with the SE. Values indicated by different letters are significantly different at p < 0.05.
Mentions: The level of deoxypyridinoline (DPD) is shown in Figure 3 (sham group: 83.31 ± 5.24 nmol/mmol creatinine; control: 142.34 ± 26.7 nmol/mmol creatinine; PrS: 140.82 ± 12.0 nmol/mmol creatinine; PrDS: 91.2 ± 4.04 nmol/mmol creatinine; casein: 118.05 ± 9.64 nmol/mmol creatinine). Type 1 collagen accounts for approximately 90% of the organic matrix of bone and is cross-linked by pyridinoline and DPD, which are released and excreted in the urine during bone resorption and collagen breakdown. Therefore, the presence of DPD in the urine is considered to be a marker of bone resorption [26]. The control, PrS and casein groups showed significantly higher DPD levels than the sham group. However, the DPD level of the PrDS group was almost identical to that of the sham group.

Bottom Line: The apparent calcium absorption rate decreased following ovariectomy and was not replenished by oral administration of phytate-removed soybean β-conglycinin (PrS) or casein.Markers of bone resorption, such as serum parathyroid hormone (PTH) and urinary deoxypyridinoline (DPD), increased, and the bone mineral density and breaking stress decreased following ovariectomy.However, PrDS supplementation suppressed the changes caused by the decrease in calcium absorption from the small intestine.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry and Life Science, Nihon University, 1866 Kameino, Fujisawa-shi, Kanagawa 252-0880, Japan. makao@brs.nihon-u.ac.jp.

ABSTRACT
Phytate-removed and deamidated soybean β-conglycinin (PrDS) prepared by ion-exchange resins was supplemented to be 4% in the diet administered to ovariectomized rats to investigate its preventive effect on osteoporosis. The apparent calcium absorption rate decreased following ovariectomy and was not replenished by oral administration of phytate-removed soybean β-conglycinin (PrS) or casein. On the other hand, administration of PrDS restored the calcium absorption rate to the same level as the sham group. Markers of bone resorption, such as serum parathyroid hormone (PTH) and urinary deoxypyridinoline (DPD), increased, and the bone mineral density and breaking stress decreased following ovariectomy. However, PrDS supplementation suppressed the changes caused by the decrease in calcium absorption from the small intestine. Therefore, PrDS supplementation shows promise for the prevention of postmenopausal osteoporosis.

Show MeSH
Related in: MedlinePlus