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Insecticide-mediated up-regulation of cytochrome P450 genes in the red flour beetle (Tribolium castaneum).

Liang X, Xiao D, He Y, Yao J, Zhu G, Zhu KY - Int J Mol Sci (2015)

Bottom Line: Reverse transcription quantitative PCR (RT-qPCR) revealed that CYP4G7 and CYP345A1 can be significantly up-regulated by cypermethrin (1.97- and 2.06-fold, respectively), permethrin (2.00- and 2.03-fold) and lambda-cyhalothrin (1.73- and 1.81-fold), whereas CYP4BR3 and CYP345A1 can be significantly up-regulated by imidacloprid (1.99- and 1.83-fold) when 20-day larvae were exposed to each of these insecticides at the concentration of LC20 for 24 h.Our study demonstrated that up-regulation of these CYP genes was rapid and only required low concentrations of insecticides, and the up-regulation not only depended on the CYP genes but also the type of insecticides.Our results along with those from previous studies also indicated that there were no specific patterns for predicting the up-regulation of specific CYP gene families based on the insecticide classification.

View Article: PubMed Central - PubMed

Affiliation: Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310029, China. liangxiaozju@126.com.

ABSTRACT
Some cytochrome P450 (CYP) genes are known for their rapid up-regulation in response to insecticide exposures in insects. To date, however, limited information is available with respect to the relationships among the insecticide type, insecticide concentration, exposure duration and the up-regulated CYP genes. In this study, we examined the transcriptional response of eight selected CYP genes, including CYP4G7, CYP4Q4, CYP4BR3, CYP12H1, CYP6BK11, CYP9D4, CYP9Z5 and CYP345A1, to each of four insecticides in the red flour beetle, Tribolium castaneum. Reverse transcription quantitative PCR (RT-qPCR) revealed that CYP4G7 and CYP345A1 can be significantly up-regulated by cypermethrin (1.97- and 2.06-fold, respectively), permethrin (2.00- and 2.03-fold) and lambda-cyhalothrin (1.73- and 1.81-fold), whereas CYP4BR3 and CYP345A1 can be significantly up-regulated by imidacloprid (1.99- and 1.83-fold) when 20-day larvae were exposed to each of these insecticides at the concentration of LC20 for 24 h. Our studies also showed that similar levels of up-regulation can be achieved for CYP4G7, CYP4BR3 and CYP345A1 by cypermethrin, permethrin, lambda-cyhalothrin or imidacloprid with approximately one fourth of LC20 in 6 h. Our study demonstrated that up-regulation of these CYP genes was rapid and only required low concentrations of insecticides, and the up-regulation not only depended on the CYP genes but also the type of insecticides. Our results along with those from previous studies also indicated that there were no specific patterns for predicting the up-regulation of specific CYP gene families based on the insecticide classification.

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Up-regulation of CYP genes in T. castaneum after exposed to different insecticides. Dash lines represent relative transcript level of the control (larvae treated with the insecticide solvent only) as 1.0. The up-regulation fold was acquired by comparing the transcript levels of each CYP between the treated and the control insects. The CYP genes with a statistically significant up-regulation are marked with asterisks (Student’s t test, * p < 0.05).
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ijms-16-02078-f003: Up-regulation of CYP genes in T. castaneum after exposed to different insecticides. Dash lines represent relative transcript level of the control (larvae treated with the insecticide solvent only) as 1.0. The up-regulation fold was acquired by comparing the transcript levels of each CYP between the treated and the control insects. The CYP genes with a statistically significant up-regulation are marked with asterisks (Student’s t test, * p < 0.05).

Mentions: In order to examine which CYP genes can be significantly up-regulated by insecticides, reverse transcription quantitative PCR (RT-qPCR) was performed to determine the change of transcript level for each of the eight CYP genes after the 20-day larvae were exposed to cypermethrin, lambda-cyhalothrin, permethrin or imidacloprid at their approximate LC20 concentrations for 24 h (Figure 3). CYP345A1 was up-regulated by all the four insecticides tested, whereas CYP4G7 was up-regulated by all the three pyrethroids but not by imidacloprid. However, CYP4BR3 was up-regulated only by imidacloprid. Overall, only CYP4G7 and CYP345A1 can be up-regulated by the three pyrethroids, and only CYP4BR3 and CYP345A1 can be up-regulated by imidacloprid. The levels of the up-regulation range from 1.73–2.06-fold.


Insecticide-mediated up-regulation of cytochrome P450 genes in the red flour beetle (Tribolium castaneum).

Liang X, Xiao D, He Y, Yao J, Zhu G, Zhu KY - Int J Mol Sci (2015)

Up-regulation of CYP genes in T. castaneum after exposed to different insecticides. Dash lines represent relative transcript level of the control (larvae treated with the insecticide solvent only) as 1.0. The up-regulation fold was acquired by comparing the transcript levels of each CYP between the treated and the control insects. The CYP genes with a statistically significant up-regulation are marked with asterisks (Student’s t test, * p < 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307350&req=5

ijms-16-02078-f003: Up-regulation of CYP genes in T. castaneum after exposed to different insecticides. Dash lines represent relative transcript level of the control (larvae treated with the insecticide solvent only) as 1.0. The up-regulation fold was acquired by comparing the transcript levels of each CYP between the treated and the control insects. The CYP genes with a statistically significant up-regulation are marked with asterisks (Student’s t test, * p < 0.05).
Mentions: In order to examine which CYP genes can be significantly up-regulated by insecticides, reverse transcription quantitative PCR (RT-qPCR) was performed to determine the change of transcript level for each of the eight CYP genes after the 20-day larvae were exposed to cypermethrin, lambda-cyhalothrin, permethrin or imidacloprid at their approximate LC20 concentrations for 24 h (Figure 3). CYP345A1 was up-regulated by all the four insecticides tested, whereas CYP4G7 was up-regulated by all the three pyrethroids but not by imidacloprid. However, CYP4BR3 was up-regulated only by imidacloprid. Overall, only CYP4G7 and CYP345A1 can be up-regulated by the three pyrethroids, and only CYP4BR3 and CYP345A1 can be up-regulated by imidacloprid. The levels of the up-regulation range from 1.73–2.06-fold.

Bottom Line: Reverse transcription quantitative PCR (RT-qPCR) revealed that CYP4G7 and CYP345A1 can be significantly up-regulated by cypermethrin (1.97- and 2.06-fold, respectively), permethrin (2.00- and 2.03-fold) and lambda-cyhalothrin (1.73- and 1.81-fold), whereas CYP4BR3 and CYP345A1 can be significantly up-regulated by imidacloprid (1.99- and 1.83-fold) when 20-day larvae were exposed to each of these insecticides at the concentration of LC20 for 24 h.Our study demonstrated that up-regulation of these CYP genes was rapid and only required low concentrations of insecticides, and the up-regulation not only depended on the CYP genes but also the type of insecticides.Our results along with those from previous studies also indicated that there were no specific patterns for predicting the up-regulation of specific CYP gene families based on the insecticide classification.

View Article: PubMed Central - PubMed

Affiliation: Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310029, China. liangxiaozju@126.com.

ABSTRACT
Some cytochrome P450 (CYP) genes are known for their rapid up-regulation in response to insecticide exposures in insects. To date, however, limited information is available with respect to the relationships among the insecticide type, insecticide concentration, exposure duration and the up-regulated CYP genes. In this study, we examined the transcriptional response of eight selected CYP genes, including CYP4G7, CYP4Q4, CYP4BR3, CYP12H1, CYP6BK11, CYP9D4, CYP9Z5 and CYP345A1, to each of four insecticides in the red flour beetle, Tribolium castaneum. Reverse transcription quantitative PCR (RT-qPCR) revealed that CYP4G7 and CYP345A1 can be significantly up-regulated by cypermethrin (1.97- and 2.06-fold, respectively), permethrin (2.00- and 2.03-fold) and lambda-cyhalothrin (1.73- and 1.81-fold), whereas CYP4BR3 and CYP345A1 can be significantly up-regulated by imidacloprid (1.99- and 1.83-fold) when 20-day larvae were exposed to each of these insecticides at the concentration of LC20 for 24 h. Our studies also showed that similar levels of up-regulation can be achieved for CYP4G7, CYP4BR3 and CYP345A1 by cypermethrin, permethrin, lambda-cyhalothrin or imidacloprid with approximately one fourth of LC20 in 6 h. Our study demonstrated that up-regulation of these CYP genes was rapid and only required low concentrations of insecticides, and the up-regulation not only depended on the CYP genes but also the type of insecticides. Our results along with those from previous studies also indicated that there were no specific patterns for predicting the up-regulation of specific CYP gene families based on the insecticide classification.

Show MeSH
Related in: MedlinePlus