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Insecticide-mediated up-regulation of cytochrome P450 genes in the red flour beetle (Tribolium castaneum).

Liang X, Xiao D, He Y, Yao J, Zhu G, Zhu KY - Int J Mol Sci (2015)

Bottom Line: Reverse transcription quantitative PCR (RT-qPCR) revealed that CYP4G7 and CYP345A1 can be significantly up-regulated by cypermethrin (1.97- and 2.06-fold, respectively), permethrin (2.00- and 2.03-fold) and lambda-cyhalothrin (1.73- and 1.81-fold), whereas CYP4BR3 and CYP345A1 can be significantly up-regulated by imidacloprid (1.99- and 1.83-fold) when 20-day larvae were exposed to each of these insecticides at the concentration of LC20 for 24 h.Our study demonstrated that up-regulation of these CYP genes was rapid and only required low concentrations of insecticides, and the up-regulation not only depended on the CYP genes but also the type of insecticides.Our results along with those from previous studies also indicated that there were no specific patterns for predicting the up-regulation of specific CYP gene families based on the insecticide classification.

View Article: PubMed Central - PubMed

Affiliation: Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310029, China. liangxiaozju@126.com.

ABSTRACT
Some cytochrome P450 (CYP) genes are known for their rapid up-regulation in response to insecticide exposures in insects. To date, however, limited information is available with respect to the relationships among the insecticide type, insecticide concentration, exposure duration and the up-regulated CYP genes. In this study, we examined the transcriptional response of eight selected CYP genes, including CYP4G7, CYP4Q4, CYP4BR3, CYP12H1, CYP6BK11, CYP9D4, CYP9Z5 and CYP345A1, to each of four insecticides in the red flour beetle, Tribolium castaneum. Reverse transcription quantitative PCR (RT-qPCR) revealed that CYP4G7 and CYP345A1 can be significantly up-regulated by cypermethrin (1.97- and 2.06-fold, respectively), permethrin (2.00- and 2.03-fold) and lambda-cyhalothrin (1.73- and 1.81-fold), whereas CYP4BR3 and CYP345A1 can be significantly up-regulated by imidacloprid (1.99- and 1.83-fold) when 20-day larvae were exposed to each of these insecticides at the concentration of LC20 for 24 h. Our studies also showed that similar levels of up-regulation can be achieved for CYP4G7, CYP4BR3 and CYP345A1 by cypermethrin, permethrin, lambda-cyhalothrin or imidacloprid with approximately one fourth of LC20 in 6 h. Our study demonstrated that up-regulation of these CYP genes was rapid and only required low concentrations of insecticides, and the up-regulation not only depended on the CYP genes but also the type of insecticides. Our results along with those from previous studies also indicated that there were no specific patterns for predicting the up-regulation of specific CYP gene families based on the insecticide classification.

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Stage-dependent (A) and tissue-dependent (B) expression patterns of eight selected CYP genes in T. castaneum (Georgia-1 strain). The expression profiles were evaluated by reverse transcription PCR (RT-PCR). The expression patterns of five different tissues, including foregut (FG), midgut (MG), hindgut (HG), Malpighian tubules (MT), fat bodies (FB) were derived from 20-day larvae, and TcRPS3 was used as an internal reference gene.
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ijms-16-02078-f002: Stage-dependent (A) and tissue-dependent (B) expression patterns of eight selected CYP genes in T. castaneum (Georgia-1 strain). The expression profiles were evaluated by reverse transcription PCR (RT-PCR). The expression patterns of five different tissues, including foregut (FG), midgut (MG), hindgut (HG), Malpighian tubules (MT), fat bodies (FB) were derived from 20-day larvae, and TcRPS3 was used as an internal reference gene.

Mentions: For the stage-dependent expression pattern of the eight CYP genes, we found that almost all these genes were expressed in 20-day larvae, 5-day pupae and 3-day adults except CYP12H1 which appeared to be only expressed in 20-day larvae (Figure 2A). The CYP12H1 expression pattern is consistent with that of the insecticide resistant strain (QTC279) of T. castaneum reported by Zhu et al. [16]. On the other hand, CYP9Z5 showed high expression in larval and adult stages but very low expression in egg and pupal stages. Its high expression appeared to associate with insect feeding. In contrast, CYP12H1, CYP345A1 and CYP4Q4 did not show detectable expression in eggs. However, the remaining five CYPs were constitutively expressed in all life stages. For the tissue-dependent expression pattern, CYP12H1, CYP4Q4, CYP4BR3 and CYP9Z5 were expressed in all the examined tissues (Figure 2B), and all the eight genes were expressed in midgut, hindgut and Malpighian tubules. However, the expression was undetectable for CYP345A1, CYP4G7 and CYP6BK11 in foregut and for CYP9D4 in fat bodies.


Insecticide-mediated up-regulation of cytochrome P450 genes in the red flour beetle (Tribolium castaneum).

Liang X, Xiao D, He Y, Yao J, Zhu G, Zhu KY - Int J Mol Sci (2015)

Stage-dependent (A) and tissue-dependent (B) expression patterns of eight selected CYP genes in T. castaneum (Georgia-1 strain). The expression profiles were evaluated by reverse transcription PCR (RT-PCR). The expression patterns of five different tissues, including foregut (FG), midgut (MG), hindgut (HG), Malpighian tubules (MT), fat bodies (FB) were derived from 20-day larvae, and TcRPS3 was used as an internal reference gene.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307350&req=5

ijms-16-02078-f002: Stage-dependent (A) and tissue-dependent (B) expression patterns of eight selected CYP genes in T. castaneum (Georgia-1 strain). The expression profiles were evaluated by reverse transcription PCR (RT-PCR). The expression patterns of five different tissues, including foregut (FG), midgut (MG), hindgut (HG), Malpighian tubules (MT), fat bodies (FB) were derived from 20-day larvae, and TcRPS3 was used as an internal reference gene.
Mentions: For the stage-dependent expression pattern of the eight CYP genes, we found that almost all these genes were expressed in 20-day larvae, 5-day pupae and 3-day adults except CYP12H1 which appeared to be only expressed in 20-day larvae (Figure 2A). The CYP12H1 expression pattern is consistent with that of the insecticide resistant strain (QTC279) of T. castaneum reported by Zhu et al. [16]. On the other hand, CYP9Z5 showed high expression in larval and adult stages but very low expression in egg and pupal stages. Its high expression appeared to associate with insect feeding. In contrast, CYP12H1, CYP345A1 and CYP4Q4 did not show detectable expression in eggs. However, the remaining five CYPs were constitutively expressed in all life stages. For the tissue-dependent expression pattern, CYP12H1, CYP4Q4, CYP4BR3 and CYP9Z5 were expressed in all the examined tissues (Figure 2B), and all the eight genes were expressed in midgut, hindgut and Malpighian tubules. However, the expression was undetectable for CYP345A1, CYP4G7 and CYP6BK11 in foregut and for CYP9D4 in fat bodies.

Bottom Line: Reverse transcription quantitative PCR (RT-qPCR) revealed that CYP4G7 and CYP345A1 can be significantly up-regulated by cypermethrin (1.97- and 2.06-fold, respectively), permethrin (2.00- and 2.03-fold) and lambda-cyhalothrin (1.73- and 1.81-fold), whereas CYP4BR3 and CYP345A1 can be significantly up-regulated by imidacloprid (1.99- and 1.83-fold) when 20-day larvae were exposed to each of these insecticides at the concentration of LC20 for 24 h.Our study demonstrated that up-regulation of these CYP genes was rapid and only required low concentrations of insecticides, and the up-regulation not only depended on the CYP genes but also the type of insecticides.Our results along with those from previous studies also indicated that there were no specific patterns for predicting the up-regulation of specific CYP gene families based on the insecticide classification.

View Article: PubMed Central - PubMed

Affiliation: Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310029, China. liangxiaozju@126.com.

ABSTRACT
Some cytochrome P450 (CYP) genes are known for their rapid up-regulation in response to insecticide exposures in insects. To date, however, limited information is available with respect to the relationships among the insecticide type, insecticide concentration, exposure duration and the up-regulated CYP genes. In this study, we examined the transcriptional response of eight selected CYP genes, including CYP4G7, CYP4Q4, CYP4BR3, CYP12H1, CYP6BK11, CYP9D4, CYP9Z5 and CYP345A1, to each of four insecticides in the red flour beetle, Tribolium castaneum. Reverse transcription quantitative PCR (RT-qPCR) revealed that CYP4G7 and CYP345A1 can be significantly up-regulated by cypermethrin (1.97- and 2.06-fold, respectively), permethrin (2.00- and 2.03-fold) and lambda-cyhalothrin (1.73- and 1.81-fold), whereas CYP4BR3 and CYP345A1 can be significantly up-regulated by imidacloprid (1.99- and 1.83-fold) when 20-day larvae were exposed to each of these insecticides at the concentration of LC20 for 24 h. Our studies also showed that similar levels of up-regulation can be achieved for CYP4G7, CYP4BR3 and CYP345A1 by cypermethrin, permethrin, lambda-cyhalothrin or imidacloprid with approximately one fourth of LC20 in 6 h. Our study demonstrated that up-regulation of these CYP genes was rapid and only required low concentrations of insecticides, and the up-regulation not only depended on the CYP genes but also the type of insecticides. Our results along with those from previous studies also indicated that there were no specific patterns for predicting the up-regulation of specific CYP gene families based on the insecticide classification.

Show MeSH
Related in: MedlinePlus