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Isolation and characterization of six AP2/ERF transcription factor genes in Chrysanthemum nankingense.

Gao C, Li P, Song A, Wang H, Wang Y, Ren L, Qi X, Chen F, Jiang J, Chen S - Int J Mol Sci (2015)

Bottom Line: The transcription response of the six TFs in response to wounding, salinity and low temperature stress and treatment with abscisic acid (ABA), salicylic acid (SA) and jasmonic acid (JA) showed that CnERF1 was up-regulated by wounding and low temperature stress but suppressed by salinity stress.The transcription of CnDREB1-1, 1-2 and 1-3 was down-regulated by ABA and JA to varying degrees.CnDREB3-1 and 3-2 was moderately increased or decreased by wounding and SA treatment, suppressed by salinity stress and JA treatment, and enhanced by low temperature stress and ABA treatment.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2011104110@njau.edu.cn.

ABSTRACT
The AP2/ERF family of plant transcription factors (TFs) regulate a variety of developmental and physiological processes. Here, we report the isolation of six AP2/ERF TF family genes from Chrysanthemum nankingense. On the basis of sequence similarity, one of these belonged to the Ethylene Responsive Factor (ERF) subfamily and the other five to the Dehydration Responsive Element Binding protein (DREB) subfamily. A transient expression experiment showed that all six AP2/ERF proteins localized to the nucleus. A yeast-one hybrid assay demonstrated that CnDREB1-1, 1-2 and 1-3 all function as transactivators, while CnERF1, CnDREB3-1 and 3-2 have no transcriptional activation ability. The transcription response of the six TFs in response to wounding, salinity and low temperature stress and treatment with abscisic acid (ABA), salicylic acid (SA) and jasmonic acid (JA) showed that CnERF1 was up-regulated by wounding and low temperature stress but suppressed by salinity stress. The transcription of CnDREB1-1, 1-2 and 1-3 was down-regulated by ABA and JA to varying degrees. CnDREB3-1 and 3-2 was moderately increased or decreased by wounding and SA treatment, suppressed by salinity stress and JA treatment, and enhanced by low temperature stress and ABA treatment.

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Differential transcript abundance of the CnAP2/ERF TFs in response to (a) salinity stress; (b) abscisic acid (ABA) treatment; (c) salicylic acid (SA) treatment; (d) jasmonic acid (JA) treatment; (e) low temperature stress; and (f) wounding. Green cells indicate suppressed and red ones enhanced levels of transcript abundance compared to the relevant control. Black cells represent no significant change of transcript abundance.
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ijms-16-02052-f004: Differential transcript abundance of the CnAP2/ERF TFs in response to (a) salinity stress; (b) abscisic acid (ABA) treatment; (c) salicylic acid (SA) treatment; (d) jasmonic acid (JA) treatment; (e) low temperature stress; and (f) wounding. Green cells indicate suppressed and red ones enhanced levels of transcript abundance compared to the relevant control. Black cells represent no significant change of transcript abundance.

Mentions: Transcript abundance of CnDREB1-1 was raised by salinity stress, peaking 4 h after the stress was imposed, whereas CnDREB3-1 was up-regulated throughout the salinity stress episode, peaking at 8 h. The other four TFs were down-regulated to differing degrees; thus CnDREB1-3 was down-regulated throughout, while the abundance of CnERF1, CnDREB1-2 and 3-2 transcript recovered to the background level after some time (Figure 4a). CnDREB3-1 and 3-2 were substantially up-regulated after a 1 and 8 h exposure to ABA, but CnDREB1-1, 1-2 and 1-3 were down-regulated throughout the treatment, and CnERF1 was only marginally induced at 1 and 12 h (Figure 4b). The effect of SA treatment was to initially induce CnDREB3-1 and 3-2 transcription, but the transcript abundance then declined, only to increase again by 24 h. CnDREB1-2 and 1-3 were both strongly down-regulated, while CnDREB1-1 was induced initially and CnERF1 at a somewhat later time (Figure 4c). When subjected to JA treatment, the transcription of both CnDREB3-1 and 3-2 was largely unaffected, that of CnERF1 was increased at the end of the period and the other three TFs were all repressed to differing extents (Figure 4d). The low temperature stress induced CnDREB1-3 and 3-2 throughout the treatment, while it caused an oscillation in the transcription of CnDREB1-1 and 3-1.CnERF1 was up-regulated within the first 8 h, but later was suppressed, and CnDREB1-2 behaved similarly (Figure 4e). Wounding increased the transcript abundance of all six TFs initially, with CnDREB1-1 and 1-3 peaking at 4 h, CnERF1 and CnDREB3-2 still substantially showing up-regulation at 24 h, and CnDREB1-3, 1-1, 1-2 and 3-1 being variously suppressed towards the end of the stress episode (Figure 4f). All obtained data were displayed in Table S1.


Isolation and characterization of six AP2/ERF transcription factor genes in Chrysanthemum nankingense.

Gao C, Li P, Song A, Wang H, Wang Y, Ren L, Qi X, Chen F, Jiang J, Chen S - Int J Mol Sci (2015)

Differential transcript abundance of the CnAP2/ERF TFs in response to (a) salinity stress; (b) abscisic acid (ABA) treatment; (c) salicylic acid (SA) treatment; (d) jasmonic acid (JA) treatment; (e) low temperature stress; and (f) wounding. Green cells indicate suppressed and red ones enhanced levels of transcript abundance compared to the relevant control. Black cells represent no significant change of transcript abundance.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307348&req=5

ijms-16-02052-f004: Differential transcript abundance of the CnAP2/ERF TFs in response to (a) salinity stress; (b) abscisic acid (ABA) treatment; (c) salicylic acid (SA) treatment; (d) jasmonic acid (JA) treatment; (e) low temperature stress; and (f) wounding. Green cells indicate suppressed and red ones enhanced levels of transcript abundance compared to the relevant control. Black cells represent no significant change of transcript abundance.
Mentions: Transcript abundance of CnDREB1-1 was raised by salinity stress, peaking 4 h after the stress was imposed, whereas CnDREB3-1 was up-regulated throughout the salinity stress episode, peaking at 8 h. The other four TFs were down-regulated to differing degrees; thus CnDREB1-3 was down-regulated throughout, while the abundance of CnERF1, CnDREB1-2 and 3-2 transcript recovered to the background level after some time (Figure 4a). CnDREB3-1 and 3-2 were substantially up-regulated after a 1 and 8 h exposure to ABA, but CnDREB1-1, 1-2 and 1-3 were down-regulated throughout the treatment, and CnERF1 was only marginally induced at 1 and 12 h (Figure 4b). The effect of SA treatment was to initially induce CnDREB3-1 and 3-2 transcription, but the transcript abundance then declined, only to increase again by 24 h. CnDREB1-2 and 1-3 were both strongly down-regulated, while CnDREB1-1 was induced initially and CnERF1 at a somewhat later time (Figure 4c). When subjected to JA treatment, the transcription of both CnDREB3-1 and 3-2 was largely unaffected, that of CnERF1 was increased at the end of the period and the other three TFs were all repressed to differing extents (Figure 4d). The low temperature stress induced CnDREB1-3 and 3-2 throughout the treatment, while it caused an oscillation in the transcription of CnDREB1-1 and 3-1.CnERF1 was up-regulated within the first 8 h, but later was suppressed, and CnDREB1-2 behaved similarly (Figure 4e). Wounding increased the transcript abundance of all six TFs initially, with CnDREB1-1 and 1-3 peaking at 4 h, CnERF1 and CnDREB3-2 still substantially showing up-regulation at 24 h, and CnDREB1-3, 1-1, 1-2 and 3-1 being variously suppressed towards the end of the stress episode (Figure 4f). All obtained data were displayed in Table S1.

Bottom Line: The transcription response of the six TFs in response to wounding, salinity and low temperature stress and treatment with abscisic acid (ABA), salicylic acid (SA) and jasmonic acid (JA) showed that CnERF1 was up-regulated by wounding and low temperature stress but suppressed by salinity stress.The transcription of CnDREB1-1, 1-2 and 1-3 was down-regulated by ABA and JA to varying degrees.CnDREB3-1 and 3-2 was moderately increased or decreased by wounding and SA treatment, suppressed by salinity stress and JA treatment, and enhanced by low temperature stress and ABA treatment.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2011104110@njau.edu.cn.

ABSTRACT
The AP2/ERF family of plant transcription factors (TFs) regulate a variety of developmental and physiological processes. Here, we report the isolation of six AP2/ERF TF family genes from Chrysanthemum nankingense. On the basis of sequence similarity, one of these belonged to the Ethylene Responsive Factor (ERF) subfamily and the other five to the Dehydration Responsive Element Binding protein (DREB) subfamily. A transient expression experiment showed that all six AP2/ERF proteins localized to the nucleus. A yeast-one hybrid assay demonstrated that CnDREB1-1, 1-2 and 1-3 all function as transactivators, while CnERF1, CnDREB3-1 and 3-2 have no transcriptional activation ability. The transcription response of the six TFs in response to wounding, salinity and low temperature stress and treatment with abscisic acid (ABA), salicylic acid (SA) and jasmonic acid (JA) showed that CnERF1 was up-regulated by wounding and low temperature stress but suppressed by salinity stress. The transcription of CnDREB1-1, 1-2 and 1-3 was down-regulated by ABA and JA to varying degrees. CnDREB3-1 and 3-2 was moderately increased or decreased by wounding and SA treatment, suppressed by salinity stress and JA treatment, and enhanced by low temperature stress and ABA treatment.

Show MeSH
Related in: MedlinePlus