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Isolation and characterization of six AP2/ERF transcription factor genes in Chrysanthemum nankingense.

Gao C, Li P, Song A, Wang H, Wang Y, Ren L, Qi X, Chen F, Jiang J, Chen S - Int J Mol Sci (2015)

Bottom Line: The transcription response of the six TFs in response to wounding, salinity and low temperature stress and treatment with abscisic acid (ABA), salicylic acid (SA) and jasmonic acid (JA) showed that CnERF1 was up-regulated by wounding and low temperature stress but suppressed by salinity stress.The transcription of CnDREB1-1, 1-2 and 1-3 was down-regulated by ABA and JA to varying degrees.CnDREB3-1 and 3-2 was moderately increased or decreased by wounding and SA treatment, suppressed by salinity stress and JA treatment, and enhanced by low temperature stress and ABA treatment.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2011104110@njau.edu.cn.

ABSTRACT
The AP2/ERF family of plant transcription factors (TFs) regulate a variety of developmental and physiological processes. Here, we report the isolation of six AP2/ERF TF family genes from Chrysanthemum nankingense. On the basis of sequence similarity, one of these belonged to the Ethylene Responsive Factor (ERF) subfamily and the other five to the Dehydration Responsive Element Binding protein (DREB) subfamily. A transient expression experiment showed that all six AP2/ERF proteins localized to the nucleus. A yeast-one hybrid assay demonstrated that CnDREB1-1, 1-2 and 1-3 all function as transactivators, while CnERF1, CnDREB3-1 and 3-2 have no transcriptional activation ability. The transcription response of the six TFs in response to wounding, salinity and low temperature stress and treatment with abscisic acid (ABA), salicylic acid (SA) and jasmonic acid (JA) showed that CnERF1 was up-regulated by wounding and low temperature stress but suppressed by salinity stress. The transcription of CnDREB1-1, 1-2 and 1-3 was down-regulated by ABA and JA to varying degrees. CnDREB3-1 and 3-2 was moderately increased or decreased by wounding and SA treatment, suppressed by salinity stress and JA treatment, and enhanced by low temperature stress and ABA treatment.

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Transcriptional activation activity of the CnAP2/ERF TFs. (a) The structure of the pGBKT7-CnAP2/ERF plasmid; (b) the arrangement of yeast strains on the plate; (c) the growth of transformed yeast cells on SD/-His-Ade + 20 mg/mL X-α-gal medium. pCL1 and pGBKT7 are positive and negative controls, respectively.
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ijms-16-02052-f003: Transcriptional activation activity of the CnAP2/ERF TFs. (a) The structure of the pGBKT7-CnAP2/ERF plasmid; (b) the arrangement of yeast strains on the plate; (c) the growth of transformed yeast cells on SD/-His-Ade + 20 mg/mL X-α-gal medium. pCL1 and pGBKT7 are positive and negative controls, respectively.

Mentions: The outcome of the transient transformation of the six CnAP2/ERF TFs is shown in Figure 2. As expected for a TF, the CnAP2/ERF-GFP signal was localized predominantly in the nucleus, while the control GFP only transgene was expressed throughout the cell. Based on the yeast one hybrid assay, CnDREB1-1, 1-2 and 1-3 all showed transcription activation activity in yeast (Figure 3), while CnERF1, CnDREB3-1 and 3-2 did not.


Isolation and characterization of six AP2/ERF transcription factor genes in Chrysanthemum nankingense.

Gao C, Li P, Song A, Wang H, Wang Y, Ren L, Qi X, Chen F, Jiang J, Chen S - Int J Mol Sci (2015)

Transcriptional activation activity of the CnAP2/ERF TFs. (a) The structure of the pGBKT7-CnAP2/ERF plasmid; (b) the arrangement of yeast strains on the plate; (c) the growth of transformed yeast cells on SD/-His-Ade + 20 mg/mL X-α-gal medium. pCL1 and pGBKT7 are positive and negative controls, respectively.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307348&req=5

ijms-16-02052-f003: Transcriptional activation activity of the CnAP2/ERF TFs. (a) The structure of the pGBKT7-CnAP2/ERF plasmid; (b) the arrangement of yeast strains on the plate; (c) the growth of transformed yeast cells on SD/-His-Ade + 20 mg/mL X-α-gal medium. pCL1 and pGBKT7 are positive and negative controls, respectively.
Mentions: The outcome of the transient transformation of the six CnAP2/ERF TFs is shown in Figure 2. As expected for a TF, the CnAP2/ERF-GFP signal was localized predominantly in the nucleus, while the control GFP only transgene was expressed throughout the cell. Based on the yeast one hybrid assay, CnDREB1-1, 1-2 and 1-3 all showed transcription activation activity in yeast (Figure 3), while CnERF1, CnDREB3-1 and 3-2 did not.

Bottom Line: The transcription response of the six TFs in response to wounding, salinity and low temperature stress and treatment with abscisic acid (ABA), salicylic acid (SA) and jasmonic acid (JA) showed that CnERF1 was up-regulated by wounding and low temperature stress but suppressed by salinity stress.The transcription of CnDREB1-1, 1-2 and 1-3 was down-regulated by ABA and JA to varying degrees.CnDREB3-1 and 3-2 was moderately increased or decreased by wounding and SA treatment, suppressed by salinity stress and JA treatment, and enhanced by low temperature stress and ABA treatment.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2011104110@njau.edu.cn.

ABSTRACT
The AP2/ERF family of plant transcription factors (TFs) regulate a variety of developmental and physiological processes. Here, we report the isolation of six AP2/ERF TF family genes from Chrysanthemum nankingense. On the basis of sequence similarity, one of these belonged to the Ethylene Responsive Factor (ERF) subfamily and the other five to the Dehydration Responsive Element Binding protein (DREB) subfamily. A transient expression experiment showed that all six AP2/ERF proteins localized to the nucleus. A yeast-one hybrid assay demonstrated that CnDREB1-1, 1-2 and 1-3 all function as transactivators, while CnERF1, CnDREB3-1 and 3-2 have no transcriptional activation ability. The transcription response of the six TFs in response to wounding, salinity and low temperature stress and treatment with abscisic acid (ABA), salicylic acid (SA) and jasmonic acid (JA) showed that CnERF1 was up-regulated by wounding and low temperature stress but suppressed by salinity stress. The transcription of CnDREB1-1, 1-2 and 1-3 was down-regulated by ABA and JA to varying degrees. CnDREB3-1 and 3-2 was moderately increased or decreased by wounding and SA treatment, suppressed by salinity stress and JA treatment, and enhanced by low temperature stress and ABA treatment.

Show MeSH
Related in: MedlinePlus