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Isolation and characterization of six AP2/ERF transcription factor genes in Chrysanthemum nankingense.

Gao C, Li P, Song A, Wang H, Wang Y, Ren L, Qi X, Chen F, Jiang J, Chen S - Int J Mol Sci (2015)

Bottom Line: The transcription response of the six TFs in response to wounding, salinity and low temperature stress and treatment with abscisic acid (ABA), salicylic acid (SA) and jasmonic acid (JA) showed that CnERF1 was up-regulated by wounding and low temperature stress but suppressed by salinity stress.The transcription of CnDREB1-1, 1-2 and 1-3 was down-regulated by ABA and JA to varying degrees.CnDREB3-1 and 3-2 was moderately increased or decreased by wounding and SA treatment, suppressed by salinity stress and JA treatment, and enhanced by low temperature stress and ABA treatment.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2011104110@njau.edu.cn.

ABSTRACT
The AP2/ERF family of plant transcription factors (TFs) regulate a variety of developmental and physiological processes. Here, we report the isolation of six AP2/ERF TF family genes from Chrysanthemum nankingense. On the basis of sequence similarity, one of these belonged to the Ethylene Responsive Factor (ERF) subfamily and the other five to the Dehydration Responsive Element Binding protein (DREB) subfamily. A transient expression experiment showed that all six AP2/ERF proteins localized to the nucleus. A yeast-one hybrid assay demonstrated that CnDREB1-1, 1-2 and 1-3 all function as transactivators, while CnERF1, CnDREB3-1 and 3-2 have no transcriptional activation ability. The transcription response of the six TFs in response to wounding, salinity and low temperature stress and treatment with abscisic acid (ABA), salicylic acid (SA) and jasmonic acid (JA) showed that CnERF1 was up-regulated by wounding and low temperature stress but suppressed by salinity stress. The transcription of CnDREB1-1, 1-2 and 1-3 was down-regulated by ABA and JA to varying degrees. CnDREB3-1 and 3-2 was moderately increased or decreased by wounding and SA treatment, suppressed by salinity stress and JA treatment, and enhanced by low temperature stress and ABA treatment.

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Localization of transiently expressed CnAP2/ERF TF products in onion epidermal cells. The upper row shows the control 35S::GFP signal, and each of the lower rows the signal from one of the 35S::CnAP2/ERF-GFP transgenes. The left panel shows bright field images, the middle one green fluorescence signals detected at 488 nm and the right one the merged Green Fluorescent Protein (GFP) and bright field images. Bar: 50 μm.
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ijms-16-02052-f002: Localization of transiently expressed CnAP2/ERF TF products in onion epidermal cells. The upper row shows the control 35S::GFP signal, and each of the lower rows the signal from one of the 35S::CnAP2/ERF-GFP transgenes. The left panel shows bright field images, the middle one green fluorescence signals detected at 488 nm and the right one the merged Green Fluorescent Protein (GFP) and bright field images. Bar: 50 μm.

Mentions: The outcome of the transient transformation of the six CnAP2/ERF TFs is shown in Figure 2. As expected for a TF, the CnAP2/ERF-GFP signal was localized predominantly in the nucleus, while the control GFP only transgene was expressed throughout the cell. Based on the yeast one hybrid assay, CnDREB1-1, 1-2 and 1-3 all showed transcription activation activity in yeast (Figure 3), while CnERF1, CnDREB3-1 and 3-2 did not.


Isolation and characterization of six AP2/ERF transcription factor genes in Chrysanthemum nankingense.

Gao C, Li P, Song A, Wang H, Wang Y, Ren L, Qi X, Chen F, Jiang J, Chen S - Int J Mol Sci (2015)

Localization of transiently expressed CnAP2/ERF TF products in onion epidermal cells. The upper row shows the control 35S::GFP signal, and each of the lower rows the signal from one of the 35S::CnAP2/ERF-GFP transgenes. The left panel shows bright field images, the middle one green fluorescence signals detected at 488 nm and the right one the merged Green Fluorescent Protein (GFP) and bright field images. Bar: 50 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307348&req=5

ijms-16-02052-f002: Localization of transiently expressed CnAP2/ERF TF products in onion epidermal cells. The upper row shows the control 35S::GFP signal, and each of the lower rows the signal from one of the 35S::CnAP2/ERF-GFP transgenes. The left panel shows bright field images, the middle one green fluorescence signals detected at 488 nm and the right one the merged Green Fluorescent Protein (GFP) and bright field images. Bar: 50 μm.
Mentions: The outcome of the transient transformation of the six CnAP2/ERF TFs is shown in Figure 2. As expected for a TF, the CnAP2/ERF-GFP signal was localized predominantly in the nucleus, while the control GFP only transgene was expressed throughout the cell. Based on the yeast one hybrid assay, CnDREB1-1, 1-2 and 1-3 all showed transcription activation activity in yeast (Figure 3), while CnERF1, CnDREB3-1 and 3-2 did not.

Bottom Line: The transcription response of the six TFs in response to wounding, salinity and low temperature stress and treatment with abscisic acid (ABA), salicylic acid (SA) and jasmonic acid (JA) showed that CnERF1 was up-regulated by wounding and low temperature stress but suppressed by salinity stress.The transcription of CnDREB1-1, 1-2 and 1-3 was down-regulated by ABA and JA to varying degrees.CnDREB3-1 and 3-2 was moderately increased or decreased by wounding and SA treatment, suppressed by salinity stress and JA treatment, and enhanced by low temperature stress and ABA treatment.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2011104110@njau.edu.cn.

ABSTRACT
The AP2/ERF family of plant transcription factors (TFs) regulate a variety of developmental and physiological processes. Here, we report the isolation of six AP2/ERF TF family genes from Chrysanthemum nankingense. On the basis of sequence similarity, one of these belonged to the Ethylene Responsive Factor (ERF) subfamily and the other five to the Dehydration Responsive Element Binding protein (DREB) subfamily. A transient expression experiment showed that all six AP2/ERF proteins localized to the nucleus. A yeast-one hybrid assay demonstrated that CnDREB1-1, 1-2 and 1-3 all function as transactivators, while CnERF1, CnDREB3-1 and 3-2 have no transcriptional activation ability. The transcription response of the six TFs in response to wounding, salinity and low temperature stress and treatment with abscisic acid (ABA), salicylic acid (SA) and jasmonic acid (JA) showed that CnERF1 was up-regulated by wounding and low temperature stress but suppressed by salinity stress. The transcription of CnDREB1-1, 1-2 and 1-3 was down-regulated by ABA and JA to varying degrees. CnDREB3-1 and 3-2 was moderately increased or decreased by wounding and SA treatment, suppressed by salinity stress and JA treatment, and enhanced by low temperature stress and ABA treatment.

Show MeSH
Related in: MedlinePlus